Produksi Xilosa dari Xilan Limbah Ampas Singkong Menggunakan Bacillus subtilis, Aureobasidium pullulans, dan Penicillium janczewskii

PRODUKSI XILOSA DARI XILAN LIMBAH AMPAS SINGKONG

  • Fefpi Nur Afnifitri Wias Arianti Program Studi Magister Bioteknologi, Program Pasca Sarjana Universitas Jember, Indonesia
  • Anak Agung Istri Ratnadewi Program Studi Kimia, Fakultas MIPA, Universitas Jember, Indonesia
  • Nurhayati Nurhayati Program Studi Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember, Indonesia
  • Jay Jayus Program Studi Magister Bioteknologi, Program Pasca Sarjana; Program Studi Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember, Indonesia
DOI: https://doi.org/10.19184/j-agt.v17i02.41326

Abstract

The demand for xylose as a xylitol raw material is increasing. The resource for this material can be explored from agricultural waste such as lignocellulosic material which is widely available in nature, cheap, and renewable. The bioconversion of lignocellulose waste into xylose can use microbes, either single culture or microbial consortia. The purpose of this research is to find out the ability of Bacillus subtilis, Aureobasidium pullulans, and Penicillium janczewskii to convert xylan of cassava waste to produce xylose, under both single individual culture and its consortia. The xylose release from the cultures were measured using DNS tests followed by HPLC determination. The results showed that the single microbial culture of B. subtilis, A. pullulans, and P. janczewskii was able to produce xylose released into the culture broth which was 75.06, 81.72, and 82.00 ppm respectively, indicating the xylanolytic enzymes activities from this microorganism which have the ability to convert xylan into xylose. Unexpectedly, all the mix culture of these microbial consortia were unable to produce higher xylose, the amount of xylose released were even lower became 63.11 ppm only when B. subtilis, A. pullulans, and P. janczewskii worked together in a simultaneous culture. This finding indicated that these three microorganisms might not be able to hydrolysed the xylan synergistically. 


Keywords: bioconversion, lignocellulosic material, microbial consortia, xylanolytic enzyme

Published
2023-12-29
How to Cite
ARIANTI, Fefpi Nur Afnifitri Wias et al. Produksi Xilosa dari Xilan Limbah Ampas Singkong Menggunakan Bacillus subtilis, Aureobasidium pullulans, dan Penicillium janczewskii. JURNAL AGROTEKNOLOGI, [S.l.], v. 17, n. 02, p. 71-83, dec. 2023. ISSN 2502-4906. Available at: <https://jurnal.unej.ac.id/index.php/JAGT/article/view/41326>. Date accessed: 03 may 2024. doi: https://doi.org/10.19184/j-agt.v17i02.41326.
Citation Formats
Issue
Vol 17 No 02 (2023)
Section
Articles
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