Senyawa Daun Rasamala (Altingia excelsa Nornha) sebagai Penghambat Proliferasi Sel Kanker Lidah Manusia In Vitro

Risyandi Anwar; Arlette Setiawan; Supriatno Supriatno; Unang Supratman

doi:10.19184/stoma.v16i2.23090

Risyandi Anwar Bagian Ilmu Kedokteran Gigi Anak, Fakultas Kedokteran Gigi Universitas Muhammadiyah Semarang
Arlette Setiawan Bagian Ilmu Kedokteran Gigi Anak, Fakultas Kedokteran Gigi, Universitas Padjadjaran
Supriatno Supriatno Bagian Penyakit Mulut, Fakultas Kedokteran Gigi, Universitas Gajah Mada
Unang Supratman Jurusan Kimia, Fakultas matematika dan Ilmu Pengetahuan Alam, Universitas Padjadjaran

DOI: https://doi.org/10.19184/stoma.v16i2.23090

Abstract

Tongue cancer is a common malignant in the oral cavity and to now medically succes its no good so often treated of life. The aims of study were to identify the secondary matabolities of Rasamala leaves, to examine the compound of Rasamala leaves toward SP-C1 cell growth inhibition. True experimental laboratories design using SP- C1 oral tongue cancer cell line was used. The ethyl acetate extract was separated their secondary metabolites by various chromatographic techniques guided by antiproliferasi activity to yield five compounds, as 3,4-dihydroxy benzoic acid (1), gallic acid (2), and apigenin (3). Compounds 1-3 were tested on their antiproliferative activity against cancer cells tongue SP-C1 and showed IC 50 values of Â 100, Â 100, and Â 100 μg/mL, respectively. Data analysis using ANACOVA assay, Tukey HSD with the level of significance α=0.05. In conclusion, compound from Rasamala leaves have a strong anticancer activity in human oral tongue cancer SP-C1 through inhibit cell prolieration..