Halal Identification for Meatballs in Pasar Tambun, Bekasi, West Java By Acrylamide Gels

  • Wahyu Hidayati Universitas Muhammadiyah Prof. Dr. Hamka
  • Meta Apriaji Universitas Muhammadiyah Prof. Dr. Hamka
  • Almawati Situmorang Universitas Muhammadiyah Prof. Dr. Hamka
  • Ifany Agustian Universitas Muhammadiyah Prof. Dr. Hamka
  • Miftahul Janah Universitas Muhammadiyah Prof. Dr. Hamka

Abstract

Halal foods are important for Moslems and there is a Moslem’s duty to eat only Halal foods which is stated in Holly Qur’an. Moreover, government already made some regulations related to Halal certification which should be obeyed by food industries, including home industries. This research aimed to detect a porcine component in meatballs sold in Pasar Tradisional Tambun, Bekasi, West Java by using SDS-PAGE Here, we combined mechanical and chemistry methods to extract the proteins and compared the protein profiles on an acrylamide gel. We got specific bands of bovine and porcine proteins according to proteins bands that showed on the gel and after comparing with the samples we found that one sample had combining profiles in the extract. We concluded that three samples used in this research were halal, meanwhile, another sample containing porcine substances.
Keywords: Halal, Proteins, Bovine, Porcine, Meatballs, SDS-PAGE.

References

Alikord, M. (2017). Species Identification and Animal Authentication in Meat Products: A Review. Journal of Food Measurement and Characterization, 12: 145-155.

Brunelle, J. L., & Green, R. (2014). Coomassie blue staining. Methods in Enzymology (1st ed., Vol. 541). Elsevier Inc.

Demirhan, Y., Ulca, P., & Senyuva, H. Z. (2012). Detection of porcine DNA in Gelatine and Gelatine-containing Processed Food Products-Halal/Kosher Authentication. Meat Science, 90(3), 686689.

Hafiz, A. (2005). Principles and Reactions of protein Extraction, Purification, and Characterization. Maryland, US: CRC Press.

Hermanto, S., Saputra, F. R., & Zilhadia. (2014). Aplikasi Metode Sds-Page (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) Untuk Mengidentifikasi Sumber Gelatin Pada Kapsul Keras. UIN Syarif Hidayatullah Jakarta, 1(1), 1-59.

Hermanto, S., Sumarlin, L. O., & Fatimah, W. (2013). Differentiation of Bovine and Porcine Gelatin Based on Spectroscopic and Electrophoretic Analysis. Journal of Food and Pharmaceutical Sciences, 1(3), 68-73.

Husni,-P., Putriana, N. A., & Wicaksono, I. A. (2017). Metode Deteksi Kandungan Babi dan Alkohol dalam Eksipien Farmasi dan Produk Obat untuk Menjamin Kehalalan Sediaan Obat. Majalah Farmasetika, 2(1), 1-7.

Masiri, J., Benoit, L., Barrios-Lopez, B., Thienes, C., Meshgi, M., Agapov, A., Samadpour, M. (2016). Development and Validation of A Rapid Test System for Detection of Pork Meat and Collagen Residues. Meat Science, 121, 397-402.

Montowska, M., & Pospiech, E. (2011). Authenticity Determination of Meat and Meat Products on The Protein and DNA Basis. Food Reviews International, 27(1), 84-100.

Nakyinsige, K., Man, Y. B. C., & Sazili, A. Q. (2012). Halal Authenticity Issues in Meat and Meat Products. Meat Science, 91(3), 207-214.

Rahmati, S., Julkapli, N. M., Yehye, W. A., & Basirun, W. J. (2016). Identification of Meat Origin in Food Products–A Review. Food Control.

Sandra Hermanto, C. D. K. M. (2009). Perbedaan Profil Protein Produk Olahan (Sosis) Daging Babi dan Sapi Hasil Analisa SDS-PAGE, 181-186.

Skarpeid, H., Kvaal, K., & Hildrum, K. I. (1998). Identification of Animal Species in Ground Meat Mixtures by Multivariate Analysis of Isoelectric Focusing Protein Profiles. Electophoresis, 19, 3103-3109.

Ulca, P., Balta, H., Çaǧin, I., & Senyuva, H. Z. (2013). Meat Species Identification and Halal Authentication Using pcr Analysis of Raw and Cooked Traditional Turkish Foods. Meat Science, 94(3), 280-284.

Walker, J. M. (1996). The Protein Protocols Handbook. Humana Press. Hatfield, UK.

Zilhadia, Betha, O. S., & Ummi, C. (2014). Protein Profilesof Beef (Bos indicus), Pork (Sus domesticus) , and Sausages By Using SDS-PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) Method. J.Food Pharm.Sci, 2, 46-51.
Published
2019-07-16
How to Cite
HIDAYATI, Wahyu et al. Halal Identification for Meatballs in Pasar Tambun, Bekasi, West Java By Acrylamide Gels. Jurnal ILMU DASAR, [S.l.], v. 20, n. 2, p. 105-110, july 2019. ISSN 2442-5613. Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/9062>. Date accessed: 13 nov. 2019. doi: https://doi.org/10.19184/jid.v20i2.9062.
Section
General