Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi
Abstract
Soil bacteria could be the promising source of secondary metabolites with diverse pharmacological activities. Bacteria coded as P1 and M7 were isolated from soil obtained from Muna Island, Southeast Sulawesi. This research aimed to evaluate the antioxidant activity, cytotoxic property, and identify the chemical profile of the extract derived from soil bacteria P1 and M7. Based on 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, extract derived from P1 isolate was moderately active against ABTS free radical with IC50 of 126.22±10.82 µg/mL, and less active on DPPH (IC50: 721.95±16.59 µg/mL), while M7 extract was less active towards DPPH and ABTS. Interestingly, these extracts in the concentration of 100 µg/mL were cytotoxic to breast cancer cell (MCF-7), as tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. MCF-7 viability was decreased up to 69.83±1.46 % and 61.49±0.76 % after treated with P1 and M7 extract, respectively. The extracts were also induced membrane disruption and apoptosis of MCF-7 cells. The extracts contained phenol (9.52±0.22 and 10.41±0.09 mg EAG/g extract, for P1 and M7, respectively) and flavonoids (9.16±0.16 and 9.29±0.24 mg EQ/g extract, for P1 and M7, respectively). According to Gas Chromatography Mass Spectrometry (GC-MS) analysis, Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) that previously well known as antioxidant and cytotoxic compounds, were found in the M7 and P1 extract. This compound may play a vital role in the radical scavenging and cytotoxic properties of P1 and M7 extracts. The results of this study indicate that P1 and M7 extracts could be the potential source of antioxidant and cytotoxic compounds and need to be further developed for pharmaceutical use.
References
Ahmad S, Alrouji M, Alhajlah S, Alomeir O, Pandey RP, Ashraf MS, Safeeque A & Khan S. 2023. Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum octurnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound. Plants. 12: 1-22.
Andayani DGS, Sukandar U, Sukandar EY & Adnyana IK. 2015. Antibacterial, Antifungal and Anticancer Activity of Five Strains of Soil Microorganisms Isolated from Tangkuban Perahu Mountain by Fermentation. HAYATI Journal of Biosciences. 22(4): 186-190.
Arivo D, Mubarik NR, Rusmana I, Batubara I. 2023. Antioxidant Activity of Various Solvent Extracts from Endophytic Bacteria Isolated from Girang (Leea indica) Leaves. Biodiversitas. 24(1): 415-422.
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R & Koirala N. 2019. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants. 8(96): 1-12.
Berdowska I, Zielin´ski B, Fecka I, Kulbacka J, Saczko J & Gamian A. 2013. Cytotoxic Impact of Phenolics from Lamiaceae Species on Human Breast Cancer Cells. Food Chemistry. 141: 1313-1321.
Borin DB, Castrejón-Arroyo K, Cruz-Nolasco A, Peña-Rico M, Sagrillo MR, Santos RCV, Baco LS, Pérez-Picaso L, Camacho L & Navarro-Mtz AK. 2021. Parasporin A13-2 of Bacillus thuringiensis Isolates from the Papaloapan Region (Mexico) Induce a Cytotoxic Effect by Late Apoptosis against Breast Cancer Cells. Toxins. 9:13(7): 476.
Celladurai R & Vadamalai S. 2022. Antagonistic, Antibiofilm Antioxidant and Anticancer Activity of Secondary Metabolites of Isolated Bacteria from Mangrove Soil. International Journal of Biosciences. 21(6): 378-386.
Cruz-Ramírez SG, López-Saiz CM, Rosas-Burgos EC, Cinco-Moroyoqui FJ, Velázquez C, Hernández J & Burgos-Hernández A. 2021. Antimutagenic Bis (2-ethylhexyl) Phthalate Isolated from Octopus (Paraoctopus vulgaris). Food Science and Technology. 41(2): 314-320.
Ding T, Yang LJ, Zhang WD & Shen YH. 2019. The Secondary Metabolites of Rare Actinomycetes: Chemistry and Bioactivity. RSC Advances. 9: 21964-21988.
Dolati M, Tafvizi F, Salehipour M, Movahed TK & Jafari P. 2021. Inhibitory Effects of Probiotic Bacillus coagulans against MCF7 Breast Cancer Cells. Iranian Journal of Microbiology. 13(6): 839-847.
El Gaied HAA, Hashem AEGM, Fahmy AM & El-Rakaiby MT. 2017. Antiviral Activity of Secondary Metabolites Produced by Streptomyces Species Isolated from Egyptian Soil. International Journal of Advance Research. 5: 1165-1174.
Gopi M, Dhayanithi NB, Devi KN, Kumar TTA. 2014. Marine Natural Product, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- (C7H10N2O2) of Sntioxidant Properties from Bacillus Species at Lakshadweep Archipelago. Journal of Coastal Life Medicine. 2(8): 636-641.
Gupta C, Prakash D, Gupta S. 2014. Natural Useful Therapeutic Products from Microbes. Microbiology & Experimentation. 1(1): 30-37.
Hindersah R & Rahmi NM. 2020. Profil Komoditas Kacang Mete dan Jagung di Pulau Muna Provinsi Sulawesi Tenggara. Agrologia. 9(2): 62-70.
Imrawati, Mus S, Gani SA & Bubua KI. 2017. Uji Aktivitas Antioksidan Fraksi Etil Asetat Daun Kersen (Muntingia calabura L.) Menggunakan Metode ABTS. Journal of Pharmaceutical and Medicinal Sciences. 2(2): 59-62.
Irawan C, Putri ID, Sukiman M, Utami A, Ismail, Putri RK, Lisandi A & Pratama AN. 2022. Antioxidant Activity of DPPH, CUPRAC, and FRAP Methods, as Well as Activity of Alpha-Glucosidase Inhibiting Enzymes from Tinospora crispa (L.) Stem Ultrasonic Extract. Pharmacognosy Journal. 14(5): 511-520.
Javed MR, Salman M, Tariq A, Tawab A, Zahoor MK, Naheed S, Shahid M, Ijaz A & Ali H. 2022. The Antibacterial and Larvicidal Potential of Bis-(2-Ethylhexyl) Phthalate from Lactiplantibacillus plantarum. Molecules. 27 (21): 1-15.
Kacergius A, Sivojiene D. 2023. Microbial Diversity and Abundance in Loamy Sandy Soil under Renaturalization of Former Arable Land. Peer Journal of Life & Environment. 11: 1-17.
Kiran GS, Priyadharsini S, Sajayan A, Ravindran A & Selvin J. 2018. An Antibiotic Agent Pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro Isolated from A Marine Bacteria Bacillus tequilensis MSI45 Effectively Controls Multi-Drug Resistant Staphylococcus aureus. RSC Advances. 8: 17837-17846.
Kiran I, Özşena Ö , Çelik T, İlhanb S, Gürsu BY & Demirci F. 2013. Microbial Transformations of Isophorone by Alternaria alternata and Neurospora crassa. Natural Product Communications. 8(1): 59-61.
Leyane TS, Jere SW & Houreld NN. 2022. Oxidative Stress in Ageing and Chronic Degenerative Pathologies: Molecular Mechanisms Involved in Counteracting Oxidative Stress and Chronic Inflammation. International Journal of Molecular Science. 23:1-28.
Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D & Abete P. 2018. Oxidative Stress, Aging, and Diseases. Clinical Interventions in Aging. 13: 757-772.
Mongalo N, Soyingbe OS & Makhafola TJ. 2019. Antimicrobial, Cytotoxicity, Anticancer and Antioxidant Activities of Jatropha zeyheri Sond. roots (Euphorbiaceae). Asian Pacific Journal of Tropical Biomedicine. 9(7): 307-314.
Mushtaq H, Ganai BA & Jehangir A. 2023. Exploring Soil Bacterial Diversity in Different Micro-Vegetational Habitats of Dachigam National Park in North-Western Himalaya. Scientific Report. 13: 1-18.
Osama N, Bakeer W, Raslan M, Soliman HA, Abdelmohsen UR & Sebak M. 2022 Anti-Cancer and Antimicrobial Potential of Five Soil Streptomycetes: A Metabolomics-Based Study. Royal Society Open Science. 9: 1-17.
Peyrat LA, Tsafantakis N, Georgousaki K, Ouazzani J, Genilloud O, Trougakos IP & Fokialakis N. 2019. Terrestrial Microorganisms: Cell Factories of Bioactive Molecules with Skin Protecting Applications. Molecules. 24(1836): 1-35.
Prastya ME, Astuti RI, Batubara I, Takagi H & Wahyudi AT. 2020. Natural Extract and Its Fractions Isolated from The Marine Bacterium Pseudoalteromonas flavipulchra STILL-33 Have Antioxidant and Anti-Aging Activities in Schizosaccharomyces pombe. FEMS Yeast Research. 20:1-14.
Prastya ME, Astuti RI, Batubara I, Wahyudi AT. 2019. Antioxidant, Antiglycation and in Vivo Antiaging Effects of Metabolite Extracts from Marine Sponge-associated Bacteria. Indian Journal of Pharmaceutical Science. 81(2): 344-353.
Priyanto JA, Prastya ME, Astuti RI & Kristiana R. 2023. The Antibacterial and Antibiofilm Activities of the Endophytic Bacteria Associated with Archidendron pauciflorum against Multidrug-Resistant Strains. Applied Biochemistry and Biotechnology. Published Online.
Priyanto JA, Prastya ME, Primahana G & Randy A, Utami DT. 2023. Paederia foetida Linn Leaves-Derived Extract Showed Antioxidant and Cytotoxic Properties Against Breast Carcinoma Cell. Hayati Journal of Biosciences. 30(2): 271-280.
Rani K. 2017. Role of Antioxidants in Prevention of Diseases. Journal of Applied Biotechnology and Bioengineering. 4(1): 495-496.
Sanjenbam P & Kannabiran K. 2016. Bioactivity of Pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(phenylmethyl)- Extracted from Streptomyces sp. VITPK9 Isolated from the Salt Spring Habitat of Manipur, India. Asian Journal of Pharmaceutics. 10(4): 265-270.
Schiller CD, Schneider MR & Von Angerer E. 1990. 3-(Cyclohexenonyl)-4-(4-hydroxyphenyl)-hexanes: Antiandrogenes Derived from the Estrogen Hexestrol. Archive der Pharmazie. (Weinheim). 323(7): 417-420.
Ser HL, Palanisamy UD, Yin WF, Malek SNA, Chan KG, Goh BH, & Lee LH. 2015. Presence of Antioxidative Agent, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- in Newly Isolated Streptomyces mangrovisoli sp. nov. Frontiers in Microbiology. 6:1-11.
Żymańczyk-Duda E, Szmigie-Merena B, Brzezińska-Rodak M & Klimek-Ochab M. 2018. Natural antioxidants–properties and possible applications. Journal of Applied Biotechnology and Bioengineering. 5(4): 251-258.
Andayani DGS, Sukandar U, Sukandar EY & Adnyana IK. 2015. Antibacterial, Antifungal and Anticancer Activity of Five Strains of Soil Microorganisms Isolated from Tangkuban Perahu Mountain by Fermentation. HAYATI Journal of Biosciences. 22(4): 186-190.
Arivo D, Mubarik NR, Rusmana I, Batubara I. 2023. Antioxidant Activity of Various Solvent Extracts from Endophytic Bacteria Isolated from Girang (Leea indica) Leaves. Biodiversitas. 24(1): 415-422.
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R & Koirala N. 2019. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants. 8(96): 1-12.
Berdowska I, Zielin´ski B, Fecka I, Kulbacka J, Saczko J & Gamian A. 2013. Cytotoxic Impact of Phenolics from Lamiaceae Species on Human Breast Cancer Cells. Food Chemistry. 141: 1313-1321.
Borin DB, Castrejón-Arroyo K, Cruz-Nolasco A, Peña-Rico M, Sagrillo MR, Santos RCV, Baco LS, Pérez-Picaso L, Camacho L & Navarro-Mtz AK. 2021. Parasporin A13-2 of Bacillus thuringiensis Isolates from the Papaloapan Region (Mexico) Induce a Cytotoxic Effect by Late Apoptosis against Breast Cancer Cells. Toxins. 9:13(7): 476.
Celladurai R & Vadamalai S. 2022. Antagonistic, Antibiofilm Antioxidant and Anticancer Activity of Secondary Metabolites of Isolated Bacteria from Mangrove Soil. International Journal of Biosciences. 21(6): 378-386.
Cruz-Ramírez SG, López-Saiz CM, Rosas-Burgos EC, Cinco-Moroyoqui FJ, Velázquez C, Hernández J & Burgos-Hernández A. 2021. Antimutagenic Bis (2-ethylhexyl) Phthalate Isolated from Octopus (Paraoctopus vulgaris). Food Science and Technology. 41(2): 314-320.
Ding T, Yang LJ, Zhang WD & Shen YH. 2019. The Secondary Metabolites of Rare Actinomycetes: Chemistry and Bioactivity. RSC Advances. 9: 21964-21988.
Dolati M, Tafvizi F, Salehipour M, Movahed TK & Jafari P. 2021. Inhibitory Effects of Probiotic Bacillus coagulans against MCF7 Breast Cancer Cells. Iranian Journal of Microbiology. 13(6): 839-847.
El Gaied HAA, Hashem AEGM, Fahmy AM & El-Rakaiby MT. 2017. Antiviral Activity of Secondary Metabolites Produced by Streptomyces Species Isolated from Egyptian Soil. International Journal of Advance Research. 5: 1165-1174.
Gopi M, Dhayanithi NB, Devi KN, Kumar TTA. 2014. Marine Natural Product, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- (C7H10N2O2) of Sntioxidant Properties from Bacillus Species at Lakshadweep Archipelago. Journal of Coastal Life Medicine. 2(8): 636-641.
Gupta C, Prakash D, Gupta S. 2014. Natural Useful Therapeutic Products from Microbes. Microbiology & Experimentation. 1(1): 30-37.
Hindersah R & Rahmi NM. 2020. Profil Komoditas Kacang Mete dan Jagung di Pulau Muna Provinsi Sulawesi Tenggara. Agrologia. 9(2): 62-70.
Imrawati, Mus S, Gani SA & Bubua KI. 2017. Uji Aktivitas Antioksidan Fraksi Etil Asetat Daun Kersen (Muntingia calabura L.) Menggunakan Metode ABTS. Journal of Pharmaceutical and Medicinal Sciences. 2(2): 59-62.
Irawan C, Putri ID, Sukiman M, Utami A, Ismail, Putri RK, Lisandi A & Pratama AN. 2022. Antioxidant Activity of DPPH, CUPRAC, and FRAP Methods, as Well as Activity of Alpha-Glucosidase Inhibiting Enzymes from Tinospora crispa (L.) Stem Ultrasonic Extract. Pharmacognosy Journal. 14(5): 511-520.
Javed MR, Salman M, Tariq A, Tawab A, Zahoor MK, Naheed S, Shahid M, Ijaz A & Ali H. 2022. The Antibacterial and Larvicidal Potential of Bis-(2-Ethylhexyl) Phthalate from Lactiplantibacillus plantarum. Molecules. 27 (21): 1-15.
Kacergius A, Sivojiene D. 2023. Microbial Diversity and Abundance in Loamy Sandy Soil under Renaturalization of Former Arable Land. Peer Journal of Life & Environment. 11: 1-17.
Kiran GS, Priyadharsini S, Sajayan A, Ravindran A & Selvin J. 2018. An Antibiotic Agent Pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro Isolated from A Marine Bacteria Bacillus tequilensis MSI45 Effectively Controls Multi-Drug Resistant Staphylococcus aureus. RSC Advances. 8: 17837-17846.
Kiran I, Özşena Ö , Çelik T, İlhanb S, Gürsu BY & Demirci F. 2013. Microbial Transformations of Isophorone by Alternaria alternata and Neurospora crassa. Natural Product Communications. 8(1): 59-61.
Leyane TS, Jere SW & Houreld NN. 2022. Oxidative Stress in Ageing and Chronic Degenerative Pathologies: Molecular Mechanisms Involved in Counteracting Oxidative Stress and Chronic Inflammation. International Journal of Molecular Science. 23:1-28.
Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D & Abete P. 2018. Oxidative Stress, Aging, and Diseases. Clinical Interventions in Aging. 13: 757-772.
Mongalo N, Soyingbe OS & Makhafola TJ. 2019. Antimicrobial, Cytotoxicity, Anticancer and Antioxidant Activities of Jatropha zeyheri Sond. roots (Euphorbiaceae). Asian Pacific Journal of Tropical Biomedicine. 9(7): 307-314.
Mushtaq H, Ganai BA & Jehangir A. 2023. Exploring Soil Bacterial Diversity in Different Micro-Vegetational Habitats of Dachigam National Park in North-Western Himalaya. Scientific Report. 13: 1-18.
Osama N, Bakeer W, Raslan M, Soliman HA, Abdelmohsen UR & Sebak M. 2022 Anti-Cancer and Antimicrobial Potential of Five Soil Streptomycetes: A Metabolomics-Based Study. Royal Society Open Science. 9: 1-17.
Peyrat LA, Tsafantakis N, Georgousaki K, Ouazzani J, Genilloud O, Trougakos IP & Fokialakis N. 2019. Terrestrial Microorganisms: Cell Factories of Bioactive Molecules with Skin Protecting Applications. Molecules. 24(1836): 1-35.
Prastya ME, Astuti RI, Batubara I, Takagi H & Wahyudi AT. 2020. Natural Extract and Its Fractions Isolated from The Marine Bacterium Pseudoalteromonas flavipulchra STILL-33 Have Antioxidant and Anti-Aging Activities in Schizosaccharomyces pombe. FEMS Yeast Research. 20:1-14.
Prastya ME, Astuti RI, Batubara I, Wahyudi AT. 2019. Antioxidant, Antiglycation and in Vivo Antiaging Effects of Metabolite Extracts from Marine Sponge-associated Bacteria. Indian Journal of Pharmaceutical Science. 81(2): 344-353.
Priyanto JA, Prastya ME, Astuti RI & Kristiana R. 2023. The Antibacterial and Antibiofilm Activities of the Endophytic Bacteria Associated with Archidendron pauciflorum against Multidrug-Resistant Strains. Applied Biochemistry and Biotechnology. Published Online.
Priyanto JA, Prastya ME, Primahana G & Randy A, Utami DT. 2023. Paederia foetida Linn Leaves-Derived Extract Showed Antioxidant and Cytotoxic Properties Against Breast Carcinoma Cell. Hayati Journal of Biosciences. 30(2): 271-280.
Rani K. 2017. Role of Antioxidants in Prevention of Diseases. Journal of Applied Biotechnology and Bioengineering. 4(1): 495-496.
Sanjenbam P & Kannabiran K. 2016. Bioactivity of Pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(phenylmethyl)- Extracted from Streptomyces sp. VITPK9 Isolated from the Salt Spring Habitat of Manipur, India. Asian Journal of Pharmaceutics. 10(4): 265-270.
Schiller CD, Schneider MR & Von Angerer E. 1990. 3-(Cyclohexenonyl)-4-(4-hydroxyphenyl)-hexanes: Antiandrogenes Derived from the Estrogen Hexestrol. Archive der Pharmazie. (Weinheim). 323(7): 417-420.
Ser HL, Palanisamy UD, Yin WF, Malek SNA, Chan KG, Goh BH, & Lee LH. 2015. Presence of Antioxidative Agent, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- in Newly Isolated Streptomyces mangrovisoli sp. nov. Frontiers in Microbiology. 6:1-11.
Żymańczyk-Duda E, Szmigie-Merena B, Brzezińska-Rodak M & Klimek-Ochab M. 2018. Natural antioxidants–properties and possible applications. Journal of Applied Biotechnology and Bioengineering. 5(4): 251-258.
Published
2024-01-23
How to Cite
PRIYANTO, Jepri Agung et al.
Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi.
Jurnal ILMU DASAR, [S.l.], v. 25, n. 1, p. 7-16, jan. 2024.
ISSN 2442-5613.
Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/39244>. Date accessed: 27 apr. 2024.
doi: https://doi.org/10.19184/jid.v25i1.39244.
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