THE PROPAGATION OF ORCHID THROUGH FORMATION OF PROTOCORM LIKE BODIES (PLB) FOR SUPPORTING THE RESQUE OF Phalaenopsis ORCHID IN INDONESIA

Abstract

Indonesia is a tropical country that has large orchid germ plasm because it has tropical
forests along the equatorial regions (islands of Sumatra, Kalimantan, Sulawesi and Papua)
Recently, there has been changed become oil palm plantations and climate change, which has
caused the extinction of orchid. The Phalaenopsis orchid is an orchid that has high exotic and
commercial value. The purpose of this study was to maintain the Phalaenopsis through
propagation of Protocorm Like Bodies (PLB). This study used the treatment of different kinds
of basal medium Murashige & Skoog (MS), ½ Murashige & Skoog (1/2 MS) and Vacin& Went
(VW) and 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentrations on
growth and propagation of PLB. The results showed sequentially VW, ½ MS and MS media
provided the best plb growth and media ½ MS provides regeneration plb into a good planlet
in which shoots and roots formed. Treatment of plant growth regulator of BAP: NAA effect on
diameter of plb colony and individual size of plb with 2.1 cm and 0.2 cm, respectively.