Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production

Anak Agung Istri Ratnadewi; Andika Ade Kurniawan; Wuryanti Handayani

Anak Agung Istri Ratnadewi Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jember. Center for Development of Advanced Sciences and Technology, Universitas Jember
Andika Ade Kurniawan Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jember. Center for Development of Advanced Sciences and Technology, Universitas Jember
Wuryanti Handayani Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jember. Center for Development of Advanced Sciences and Technology, Universitas Jember

Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 ^oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among the other XOS X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm).

Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase