Sintesis dan Uji Aktivitas Antibakteri Senyawa N-Fenil-4-Bromobenzamida (Synthesis and Antibacterial Activity Assay of N-Phenyl-4-Bromobenzamide)

Abstract

Infectious diseases are contagious disease caused by pathogenic microorganisms and can be spread directly or indirectly from one person to another. The aim of this research was to synthesized N-phenyl-4-bromobenzamide as a new antibacterial agent. The
reaction was carried out by reacting 1,3-diphenylthiourea with 4-bromobenzoyl chloride by nucleophilic substitution. The purification of this compound was confirmed by TLC and the structure was identified by UV, IR, 1H-NMR and 13C-NMR. The newly synthesized compound was screened for its antibacterial activity against gram-positive and gram-negative bacteria, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853 using well diffusion method. The concentration of the test solution used were 31.25; 62.5; 125; 250; 500; 1,000; 2,000; 4,000; 8,000; and 10,000 μg/ml with levofloxacin as positive control. The synthesized product did not  show any antibacterial activity which characterized by the absence of inhibition zone.
Keywords: 1,3-diphenylthiourea, N-phenyl-4-bromobenzamide, nucleophilic substitution, antibacterial activity.