Sintesis dan Uji Aktivitas Antibakteri Senyawa N-Fenil-4-Bromobenzamida (Synthesis and Antibacterial Activity Assay of N-Phenyl-4-Bromobenzamide)

  • Nazilatul Maghfiroh Fakultas Farmasi Universitas Jember
  • Indah Purnama Sary Fakultas Farmasi Universitas Jember
  • Dwi Koko Pratoko Fakultas Farmasi Universitas Jember

Abstract

Infectious diseases are contagious disease caused by pathogenic microorganisms and can be spread directly or indirectly from one person to another. The aim of this research was to synthesized N-phenyl-4-bromobenzamide as a new antibacterial agent. The
reaction was carried out by reacting 1,3-diphenylthiourea with 4-bromobenzoyl chloride by nucleophilic substitution. The purification of this compound was confirmed by TLC and the structure was identified by UV, IR, 1H-NMR and 13C-NMR. The newly synthesized compound was screened for its antibacterial activity against gram-positive and gram-negative bacteria, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853 using well diffusion method. The concentration of the test solution used were 31.25; 62.5; 125; 250; 500; 1,000; 2,000; 4,000; 8,000; and 10,000 μg/ml with levofloxacin as positive control. The synthesized product did not  show any antibacterial activity which characterized by the absence of inhibition zone.
Keywords: 1,3-diphenylthiourea, N-phenyl-4-bromobenzamide, nucleophilic substitution, antibacterial activity.

Published
2017-05-26
How to Cite
MAGHFIROH, Nazilatul; SARY, Indah Purnama; PRATOKO, Dwi Koko. Sintesis dan Uji Aktivitas Antibakteri Senyawa N-Fenil-4-Bromobenzamida (Synthesis and Antibacterial Activity Assay of N-Phenyl-4-Bromobenzamide). Pustaka Kesehatan, [S.l.], v. 5, n. 2, p. 377-381, may 2017. ISSN 2721-3218. Available at: <https://jurnal.unej.ac.id/index.php/JPK/article/view/5868>. Date accessed: 12 dec. 2024. doi: https://doi.org/10.19184/pk.v5i2.5868.
Section
Articles