Daya Hambat Ekstrak Buah Delima Merah (Punica granatum Linn) Terhadap Pertumbuhan Porphyromonas gingivalis (The Inhibition of Red Pomegranate Fruit Extract (Punica granatum Linn) on The Growth Porphyromonas gingivalis)

Abstract

Background : Halitosis is a common complaint among general population. Porphyromonas
gingivalis 33277 is the most frequent bacteria that cause halitosis. Pomegranate (Punica
granatum Linn) extract is used to control the number of Porphyromonas gingivalis population.
Bioactive compound of pomegranate is polyphenol (Flavonoids and its derivate, tanins,
ellagitanins) and Alkaloids. Objective : To Analyze the effectiveness of pomegranate against
Inhibition of Porphyromonas gingivalis Materials and Metdhods :The research used disc
diffusion method (Kirby-Bauer) with 7 treatment group (n=6). Petridish was filled with Agar and
inoculated by Porphyromonas gingivalis. after 5 minutes, set up disk that previously immersed in
100%, 50%, 25%, 12,5%, 6,25% pomegranate extract, distilled water (negative control) and
Clorhexidine 0,2% (positive control) on top. The Petridish were then placed in the desiccator and
incubator at 37oC for 24 hours. Zone of Inhibition were measured using digital caliper by three
independent observer and recorded in millimeter. The result of this study showed that there were
Inhibition zone in all treatment group (except negative control). Results and Conclusions :
Pomegranate extract had antibacterial activity against Porphyromonas gingivalis at 6,25 minimum
inhibitory concentration.
Keywords: Antibacterial activity, Punica granatum Linn extract, Porphyromonas gingivalis, Kirby-
Bauer disk diffusion method.