The Polymorphic Gene of Single Nucleotide Polymorphism (SNP) of Phytoene Synthase (PSY) to Characterize Carotenoids Yellow Root Cassava
Cassava (Manihot esculenta Crantz.) is a carbohydrate sources containing a limited amount of micronutrients, but some genotypes contain β-carotene as the precursor of vitamin A in the storage roots and leaves. Improvement of β-caroteneand minerals such as Fe / Zn content of cassava’s nutrition is mostly through by biofortification program. The storage root of β-carotene recognized by a yellow or yellowish color while the apical shoots with red to purplish. β-carotenein carotenoid biosynthetic pathway is an expression of the phytoene synthase (PSY) gene. The MePSY2 gene, one of the three MePSY family is the key gene to characterize carotenoids related gene in cassava. In this study, sequencing of the two cassava fulllenght PSY genomic DNA was carried out in conserved areas in the PSY gene region (PSY1 and PSY2) from the DNA of the cassava leaves. Adira1, Carvita25 and Ubi Kuning are yellow root storage genotypes (K1, K2 and K3) while Adira4 and Menti are white root storage genotypes (P1 and P2). Carvita25 is induced somaclonal variant of the Adira4 genotype. Contiq and consensus of nucleotide base sequences from the five cassava genotypes and CM3306-4 cultivars (acc GU111715.1) as references were analysed using the lasergene DNASTAR sequence analysis program. The results of the alignment of the base sequence constituent of the MePSY2 gene showed that the PSY2 gene with amplified genome length was 2,380 base pairs (bp) consisting of 1,140 bp exon region and 1,240 bp intron region. In the conserved coding region, there was a difference of one nucleotide base, that is, base C in two white tuber cassava genotypes replaced with A in three yellow tuber cassava genotypes in the 1.485 base (C1.485A). The SNP converts the amino acid (aa) alanine (A) to aspartic acid (D) at the 191th (A191D). Single Nucleotide polymorphism in conserved coding region can be used further as carotenoid marker for plant breeding of yellow root cassava.
Keywords: β carotene, PSY gene, polymorphic gene SNP, yellow root cassava.
Eldahshan, O. A, Singab, A. N. B. 2013. Carotenoids. Journal of Pharmacognosy and Phytochemistry 2 (1): 225-234.
Flowerika., Alok, A., Kumar, J., Thakur, N., Pandey, A., Pandey, A. K., Upadhyay, S. K, Tiwari, S. 2016. Characterization and Expression Analysis of Phytoene Synthase From Bread Wheat (Triticum aestivum L.). PloS ONE 11(10):e0162443. doi:10.1371/journal.pone.0162443
Fraser, P. D., Bramley, P. M. 2004. The Biosynthesis and Nutritional Uses of Carotenoids. Progress in Lipid Research 43: 228-265. doi: 10.1016/j.plipres.2003.10.002 PMID: 15003396
Giuliano, G. 2014. Plant Carotenoids: Genomics Meets Multi-gene Engineering. Current Opinion in Plant Biology 19: 111-117. doi: org/10.1016/j.pbi.2014.05.006
Hall, T. A. 1999. BioEdit; a User-friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95-98.
Harjes, C. E., Rocheford, T. R., Bai, L., Brutnell, T. P., Kandianis, C. B., Sowinski, S. G., Stapleton, A. E., Vallabhaneni, R., Williams, M., Wurtzel, E.T., et al. 2008. Natural Genetic Variation in Lycopene Epsilon Cyclase Tapped For Maize Biofortification. Science 319:330-333.
Jehan, T., Lakhanpaul, S. 2006. Single Nucleotide Polymorphism (SNP)-Methods and Applications in Plant Genetics: A review. Indian Journal of Biotechnology 5(4): 435-459.
Luo, X., Tomlins, K. I., Carvalho, L. J. C. B., Li, K., Chen, S. 2018. The Analysis of Candidate Genes and Loci Involved With Carotenoid Metabolism in Cassava (Manihot esculenta Crantz.) Using SLAF-seq. Acta Physiologiae Plantarum 40: 66. doi.org/10.1007/s11738-018-2634-7
Sankari, M., Rao, P. R., Hemachandran, H., Pullela, P. K., Doss, C. G. P., Tayubi, I. A., Subramanian, B., Gothandam, K. M, Singh, P., Ramamoorth, S. 2018. Prospects and Progress in The Production of Valuable Carotenoids: Insights From Metabolic Engineering, Synthetic Biology, and Computational Approaches. Journal of Biotechnology 266: 89-101.
Singh, P., Goyal, G. K. 2008. Dietary Lycopene: Its Properties and Anticarcinogenic Effects. Compr Rev Food Sci Food Saf. 7: 255-270. doi: 10.1111/j.1541-4337.2008.00044.x
Sugiyama, A., Ikoma, Y., Fujii, H., Endo, T., Nesumi, H., Shimada, T., Omura, T. 2017. Allelic Diversity of Phytoene Synthase Gene Influences the Transcription Level in Citrus Fruit Among A Citrus F1 Hybrid Population. Breeding Sciences 67: 382-392.
Welsch, R., Arango, J., Bär, C., Salazar, B., Al-Babili, S., Beltrán, J., Chavarriaga, P., Ceballos, H., Tohme, J., Beyer, P. 2010. Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by A Single Nucleotide Polymorphism in A Phytoene Synthase Gene. The Plant Cell 22: 3348-3356.
Wong, J. C., Lambert, R. J., Wurtzel, E. T., Rocheford, T. R. 2004. QTL and Candidate Genes Phytoene Synthase and ζ-carotene Desaturase Associated With The Accumulation of Carotenoids in Maize. Theor. Appl. Genet. 108: 349–359.
Yuan, H., Zhang, J., Nageswaran, D., Li, L. 2015. Carotenoid Metabolism and Regulation in Horticultural Crops. Horticulture Research 2: 15036. doi:10.1038/hortres.2015.36.
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