Adulticide Efficacy of Artemisia vulgaris L. against Aedes aegypti L.

Vika Ichsania Ninditya; Endah Purwati; Ajeng Tyas Utami; Aprillyani Sofa Marwaningtyaz; Nadia Khairunnisa Fairuz; Penny Humaidah Hamid

doi:10.19184/jid.v20i2.8174

Vika Ichsania Ninditya Faculty of Veterinary Medicine, Universitas Gadjah Mada
Endah Purwati Faculty of Veterinary Medicine, Universitas Gadjah Mada
Ajeng Tyas Utami Faculty of Veterinary Medicine, Universitas Gadjah Mada
Aprillyani Sofa Marwaningtyaz Faculty of Pharmacy, Universitas Gadjah Mada
Nadia Khairunnisa Fairuz Faculty of Pharmacy, Universitas Gadjah Mada
Penny Humaidah Hamid Department of Parasitology, Veterinary Medicine, Universitas Gadjah Mada

DOI: https://doi.org/10.19184/jid.v20i2.8174

Abstract

Aedes aegypti is the vector of various arthropod-borne diseases such as dengue fever, chikungunya and currently, zika. This study aimed to evaluate Artemisia vulgarisas other adulticides for controlling Ae. aegypti. Indonesian ministry of health hasbeen reported that more than 70.000 dengue fever human cases have occurred in 2015 covering 34 provinces which mean that dengue fever has spread now into all national territories. Eradication of Aedes still largely depends on insecticides, which is the most cost-effective strategy, and often inefficient due to resistance development in exposed Aedes population This study was designed to use of Centers for Disease Control and Prevention (CDC) bioassay standard. CDC bottles were coated with the ethanolic solution of extract with a concentration of 10, 50, 100, 500, 1000, 5000, 10000, 50000, and 100000 g per bottle. F0 mosquitoes were used for all experiments. Death and surviving mosquitoes were evaluated based on CDC standard assay. The test was performed with 10-25 adult mosquitoes every bottle and each concentration was repeated in triplicates. The results showed that LC₅₀ and LC₉₀ were 5790 g and 52110 g respectively after 120 minutes exposure to the extract. There was no mortality in ethanol control group. A. vulgaris significantly (P < 0.05) have adulticidal activity against Ae. aegypti. However, A. vulgaris have been reported to have larvacidal activity against Ae. Aegypti. These results indicated clearly that A. Vulgaris might act as the candidate of bioinsecticides for controlling Ae. aegypti.
Keywords: Aedes aegypti, Artemisia vulgaris, adulticide,bio insecticide.

References

Ahmad I., Astari S, & Tan M. 2007. Resistance of Aedes aegypti in 2006 to Pyrethroid Insecticide in Indonesia and its Association with Oxidase and Esterase Levels. Pakistan Journal of Biological Science. 10: 3688-3692.

Ajaegbu EE., Danga SP, Chijoke IU, & Okoye FB. 2016. Mosquito Adulticidal Activity of the Leaf Extracts of Spondiasmombin L. against Aedesaegypti L. and Isolation of Active Principles. J Vector Borne Dis. 53: 17-22.

Amerasan D, Murugan K, Kovendan K, Mahesh-Kumar P, Panneerselvam C, Subramaniam J, William SJ, Hwang JS. 2012. Adulticidal and Repellent Properties of Cassia tora Linn. (Family: Caesalpinaceae) against Culexquinque fasciatus, Aedesaegypti, and Anopheles stephensi.Parasitol Res,111: 1953-64.

Becker N, Petric D, Zgomba M, Boase C, Dahl C, & Madon M. 2010. Mosquitoes and Their Control. Berlin Heidelberg: Springer.

Cheah SX, Tay JW, Chan LK & Jaa lZ. 2013. Larvicidal, oviposition, and ovicidal effects of Artemisia annua (Asterales: Asteraceae) against Aedesaegypti, Anopheles sinensis, and Culexquinque fasciatus (Diptera: Culicidae). Parasitology Research.112: 3275-3282.

Elumalai D, Hemavathi M, Hemalatha P, Deepaa CV & Kaleena PK. 2016. Larvicidal activity of catechin isolated from Leucasaspera againstAedes aegypti, Anopheles stephensi, and Culexquinque fasciatus (Diptera: Culicidae). Parasitology Research. 115: 1203-1212.

Govindarajan M, Mathivanan T, Elumalai K,. Krishnappa K & Anandan A. 2011. Mosquito larvicidal, ovicidal, and repellent properties of botanical extracts against Anopheles stephensi, Aedesaegypti, and Culexquinque fasciatus (Diptera: Culicidae). Parasitology Research. 109: 353-367.

Hamid PH, Prastowo J, Widyasari A, Taubert A & Hermosilla C. 2017. Knockdown resistance (kdr) of the voltage-gated sodium channel gene of Aedesaegypti population in Denpasar, Bali, Indonesia. Parasites & Vectors, 10: 238.

Hardjanti A, Indrawati I, Donanti E, Wibowo H & Zulhasril Z. 2015. Detection of Insecticide Resistance in Aedesaegypti to Organophosphate in Pulogadung, East Jakarta. Makara J. Health Res.19:117-120.

Ishak IH, Jaal Z, Ranson H & Wondji CS.2015. Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedesaegypti and Aedesalbopictus from Malaysia. Parasit Vectors. 8: 181.

Judžentienė A & Buzelyte J. 2006. Chemical composition of essential oils of Artemisia vulgaris L. (mugwort) from North Lithuania. CHEMIJA. 17: 12–15.

Saavedra-Rodriguez K, Urdaneta-Marquez L., Rajatileka S., Moulton M., Flores AE., Fernandez-Salas I., Bisset J, Rodriguez M, McCall PJ, Donnelly MJ, Ranson H, Hemingway J, Black WC. 2007. A Mutation in The Voltage-gated Sodium Channel Gene Associated with Pyrethroid Resistance in Latin American Aedesaegypti. Insect Mol Biol. 16: 785-98.

Li CX, Kaufman PE., XueR D,. Zhao MH, Wang G, Yan T, Guo XX,. ZhangY M,. Dong YD, Xing D,. Zhang HD, Zhao TY. 2015. Relationship Between Insecticide Resistance and kdr Mutations in The Dengue Vector Aedesaegypti in Southern China. Parasites & Vectors.8: 325.

Lima EP, Paiva MH, de Araujo AP, da Silva E V, da Silva UM, de Oliveira LN, Santana AE, Barbosa CN, de Paiva Neto CC, Goulart MO, Wilding CS, Ayres CF, de Melo Santos MA. 2011. Insecticide resistance in Aedesaegypti populations from Ceara, Brazil. Parasit Vectors.4: 5.

Masotti V, Jong LD, Moreau X, Rabier J, Laffont-Schwob I & Thie´ry A. 2012. Larvicidal activity of extracts from Artemisia species against Culexpipiens L. mosquito: Comparing endemic versus ubiquist species for effectiveness. Comptes Rendus Biologies. 335: 19-25.

Murugan KP, Mahesh Kumar, Kovendan K, Amerasan D, Subrmaniam J &. Hwang JS. 2012. Larvicidal, pupicidal, repellent and adulticidal activity of Citrus sinensis orange peel extract against Anopheles stephensi, Aedesaegypti and Culexquinque fasciatus (Diptera: Culicidae). Parasitol Res,111: 1757-69.

Mya MM, Oo NN, Ha T, Oo AW, Htay TM, New CT, S. Thaung, Y. M. M. Maung, 2016. Larvicidal effect of Artemisia vulgaris leaves, flower and leaves essential oil extracts against Aedes aegypti larvae. Journal of Biological Engineering Research and Review. 3(2): 25-34.

Panneerselvam C, Murugan K, Kovendan K & Kumar MP. 2012. Mosquito larvicidal, pupicidal, adulticidal, and repellent activity of Artemisia nilagirica (Family: Compositae) against Anopheles stephensi and Aedesaegypti. Parasitol Res. 111: 2241-2251.

Ramkumar G, Karthi S, Muthusamy R, Natarajan D & Shivakumar MS. 2015. Insecticidal and Repellent Activity of Clausenadentata (Rutaceae) plant extracts against Aedesaegypti and Culexquinque fasciatus mosquitoes (Diptera: Culicidae). Parasitol Res, 114: 1139-44.

Samuel PP & Tyagi BK. 2006. Diagnostic Methods for Detection & Isolation of Dengue Viruses from Vector Mosquitoes. Indian J Med Res. 123: 615-628.

Sayono S., Hidayati AP, Fahri S, Sumanto D, Dharmana E, Hadisaputro S, Asih PBS & Syafruddin D. 2016. Distribution of Voltage-Gated Sodium Channel (Nav) Alleles among the Aedesaegypti Populations In Central Java Province and Its Association with Resistance to Pyrethroid Insecticides. PLoS One.11:150577.

Subsuebwong T, Attrapadung S, Potiwat R & Komalamisra N. 2016. Adulticide efficacy of essential oil from Piper retrofractum Vahl against Aedesaegypti and Culexquinque fasciatus. Tropical Biomedicine 33(1):84–87.

Sundararajan B & Kumari BDR. 2017. Novel synthesis of gold nanoparticles using Artemisia vulgaris L. leaf extract and their efficacy of larvicidal activity against dengue fever vector Aedesaegypti L. J Trace Elem Med Biol, 43: 187-196.

Tabanca N, Demirci B, Blythe EK, Bernier UR, Ali A, Wedge DE, Khan IA, Başer KHC. 2011. Composition of Artemisia abrotanum and A. Pontica Essential Oils and Their Repellent Activity against Aedesaegypti. Planta Med,.77: 52.

Wuliandari JR, Lee SF, White VL, Tantowijoyo W, Hoffmann AA & Endersby-Harshman NM. 2015. Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedesaegypti from Yogyakarta, Indonesia. Insects. 6: 658-85.