The Utilization of Serratia marcescens to Increase Phosphate-Availability and Production of Cucumber in Alfisols
Abstract
Serratia marcescens is a bacterium that can dissolve phosphate (P) in soil, increasing phosphate availability and allowing plants to use it to support plant growth. P-available in the soil is generally low, including in Alfisols, due to the formation of bonds with Al, Fe, Ca, Mg, and other cations, which is influenced by soil pH or clay mineral adsorption. Cucumber plants require adequate nutrients, including P nutrients, for optimal growth and production. The study was carried out in a greenhouse using a completely randomized design (CRD) with four treatments and five replications. The treatments were S. marcescens bacterial solution concentrations of P0 (0 ml); P1 (20 ml); and P2 (20 ml); P3 (60 ml) bacterial colony density: 33x109 cfu/ml. According to the findings of the study, S. marcescens inoculation had a significant effect on increasing soil pH by 6,16, P-total concentration of 41,62 mg/100g, P-available concentration of 28,39 ppm, P-tissue concentration shoot and root were 0,30% and 0,78%, respectively, and P-root uptake of 2,27 g/pot. Similarly, S. marcescens inoculation significantly affected the plant height of 138,72 cm, root volume of 43 ml, and root length of 59,92 cm compared to the control treatment.
References
Aditya M, Idwar & Nurbaiti. 2015. Aplikasi Bakteri Pelarut Fosfat Isolat No. 68 dengan Berbagai Takaran Batuan Fosfat pada Medium Gambut dalam Meningkatkan Pertumbuhan dan Produksi Kacang Hijau (Vigna radiata L.) Varietas 129. JOM Faperta. 2(2): 1-15.
Adiwijaya HD. 2019. Pengaruh Kombinasi Takaran Pupuk Nitrogen dan Pupuk Fosfat terhadap Pertumbuhan dan Hasil Tanaman Mentimun (Cucumis sativus L.) Kultivar Zatavy F1. Jurnal Agrorektan. 6(2): 2-21.
Alvernia P, Minardi S & Suntoro S. 2017. Zeolite and Organic Fertilizer Application to The Improvement of Available P and Soybean (Glycine max L) Seed Yield in Alfisols. Sains Tanah - Journal of Soil Science and Agroclimatology. 14(2): 84-90.
Alzate Zuluaga MY, Martinez de Oliveira AL, Valentinuzzi F, Tiziani R, Pii Y, Mimmo T, & Cesco S. 2021) Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants? Frontiers in Plant Science, 12:1-18.
Bertua, Irianto, & Ardiyaningsih. 2012. Pengaruh Dosis Pupuk Kandang Ayam terhadap Pertumbuhan dan Hasil Mentimun (Cucumis sativus L.) pada Tanah Ultisol. Bioplantae. 1(4): 266-273.
Cahyani P, Wijanarka & Raharjo B. 2017. Aktivitas Spesifik Selulase Serratia marcescens dengan Variasi Konsentrasi Amonium Sulfat ((NH4)2SO4) dan pH. Jurnal Biologi. 6(2): 41-49.
Cahyono O. 2018. Respon Beberapa Varietas Padi Sawah terhadap Cara Aplikasi Pupuk Fosfat di Tanah Alfisol. Jurnal Ilmiah Agrineca. 18(2): 22-33.
Campos CNS, Teixeira GCM, Prado RdeM, Caione G, da Silva Júnior GB, David CHO de, Sales AC, Roque CG & Teodoro PE. 2021. Macronutrient Deficiency in Cucumber Plants: Impacts in Nutrition, Growth and Symptoms. Journal of Plant Nutrition, 44(17): 2609-2626.
Chakraborty U, Chakraborty BN & Chakraborty AP. 2010. Influence of Serratia marcescens TRS-1 on Growth Promotion and Induction Of Resistance in Camellia Sinensis Against Fomes Lamaoensis. Journal of Plant Interactions. 5(4): 261-272.
Chen YP, Rekha PD, Arun AB, Shen FT, Lai W A & Young CC. 2006. Phosphate Solubilizing Bacteria from Subtropical Soil dnd Their Tricalcium Phosphate Solubilizing Abilities. Applied Soil Ecology. 34(1): 33-41.
Delfim J, Gerding M & Zagal E. 2020. Phosphorus fractions in Andisol and Ultisol inoculated with Bacillus thuringiensis and phosphorus uptake by wheat. Journal of Plant Nutrition. 43(18): 2728-2739.
Dhani H, Wardati & Rosmimi. 2014. Pengaruh Pupuk Vermikompos pada Tanah Inceptisol terhadap Pertumbuhan dan Hasil Sawi Hijau (Brassica juncea L.). JOM Faperta, 1(1): 1-11.
Fikdalillah, Basir M & Wahyudi I. 2016. Pengaruh Pemberian Pupuk Kandang Sapi terhadap Serapan Fosfor dan Hasil Tanaman Sawi Putih (Brassica pekinensis) pada Entisols Sidera. J. Agrotekbis. 4(5): 491-499.
Ginting EN, Pradiko I, Farrasati R & Rahutomo S. 2020. Pengaruh Rock Phosphate dan Dolomit terhadap Distribusi Perakaran Tanaman Kelapa Sawit pada Tanah Ultisols. Jurnal Agrikultura. 31(1): 32-41.
Hartatik W & Idris DK. 2008. Kelarutan Fosfat Alam dan SP-36 dalam Gambut yang Diberi Bahan Amelioran Tanah Mineral. Jurnal Tanah dan Iklim, 27: 45-56.
Kadarwati FT. 2016. Evaluasi Kesuburan Tanah untuk Pertanaman Tebu di Kabupaten Rembang, Jawa Tengah. Jurnal Penelitian Tanaman Industri. 22(2): 53-62.
Kannan P, Paramasivan M, Marimuthu S, Swaminathan C & Bose J. 2021. Applying Both Biochar and Phosphobacteria Enhances Vigna mungo L. Growth and Yield in Acid Soils by Increasing Soil pH, Moisture Content, Microbial Growth and P Availability. Agriculture, Ecosystems and Environment, 308: 1-9.
Kaur R & Kaur S. 2020. Variation in the Phosphate Solubilizing Bacteria from Virgin and the Agricultural Soils of Punjab. Current Microbiology. 77(9): 2118-2127.
Khan MA, Khatun A & Islam T. 2016. Promotion of Plant Growth by Phytohormone Producing Bacteria. In Industrial Microbiology: Microbes in Action (pp. 1-42).
Kurniawan AR, Bisri M & Suhartanto E. 2019. Analisis Kekeringan pada Daerah Aliran Sungai (DAS) Bedadung Berbasis Sistem Informasi Geografis (SIG). Jurnal Teknik Pengairan. 10(2): 97-109.
Ligowe IS, Young SD, Ander EL, Kabambe V, Chilimba ADC, Bailey EH, Lark RM & Nalivata PC. 2020. Agronomic biofortification of leafy vegetables grown in an Oxisol, Alfisol and Vertisol with isotopically labelled selenium (77Se). Geoderma. 361: 1-11.
Lovitna G, Nuraini Y & Istiqomah N. 2021. Pengaruh Aplikasi Bakteri Pelarut Fosfat dan Pupuk Anorganik Fosfat Terhadap Populasi Bakteri Pelarut Fosfat, P-Tersedia, dan Hasil Tanaman Jagung pada Alfisol. Jurnal Tanah Dan Sumberdaya Lahan. 8(2): 437-449.
Marra LM, Maria de Oliveira S, Roberto Fonsêca Sousa Soares C, Maria de Souza Moreira F & Borges Regitano J. 2011. Solubilisation of Inorganic Phosphates by Inoculant Strains From Tropical Legumes. Sci. Agric. 68(5): 603-609.
Marra LM, Sousa Soares CRF, de Oliveira SM, Ferreira PAA, Soares BL, de Carvalho RF, de Lima JM & de Moreira FMS. 2012. Biological Nitrogen Fixation and Phosphate Solubilization by Bacteria Isolated from Tropical Soils. Plant and Soil. 357(1): 289-307.
Mohamed EAH, Farag AG & Youssef SA. 2018. Phosphate Solubilization by Bacillus subtilis and Serratia marcescens Isolated from Tomato Plant Rhizosphere. Journal of Environmental Protection. 09(03): 266–277.
Mohammed WS, Mishra KS, Singh RK, Singh, MK & Soni SS. 2021. The effect of NPK on The Growth, Yield and Quality of Cucumber (Cucumis sativus L.) Under Protected cultivation. Journal of Pharmacognosy and Phytochemistry. 10(1): 2011-2014.
Nursyamsi D & Setyorini DD. 2009. Ketersediaan P Tanah-Tanah Netral dan Alkalin. Jurnal Tanah Dan Iklim, 30: 25-36.
Oksilia & Alby S. 2020. Pengaruh Pupuk Fosfat terhadap Pertumbuhan dan Produksi Mentimun Jepang (Cucumis sativus L.). Jurnal Ilmu Pertanian Agronitas. 2(2): 38-45.
Patel K, Patel S, Parekh V & Jha S. 2016. Isolation and Characterization of Salt Tolerant Phosphate Solubilizing Serratia marcescens Isolated from Coastal Area. Journal of Pure and Applied Microbiology, 10(3): 2401-2408.
Rawat P, Das S, Shankhdhar D & Shankhdhar, SC. 2021. Phosphate-Solubilizing Microorganisms: Mechanism and Their Role in Phosphate Solubilization and Uptake. Journal of Soil Science and Plant Nutrition. 21: 49-68.
Setyawan F. 2017. Pengaruh Bacillus subtilis dan Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Kedelai (Glycine max L.). Jurnal Hijau Cendekia. 2(1): 21-28.
Shirmohammadi E, Alikhani HA, Pourbabaei AA & Etesami H. 2020. Improved Phosphorus (P) Uptake and Yield of Rainfed Wheat Fed with P Fertilizer by Drought-Tolerant Phosphate-Solubilizing Fluorescent Pseudomonads Strains: a Field Study in Drylands. Journal of Soil Science and Plant Nutrition. 20(4): 2195-2211.
Shrivastava M, Srivastava PC & D’Souza SF. 2018. Phosphate-solubilizing microbes: Diversity and phosphates solubilization mechanism. In Role of Rhizospheric Microbes in Soil: Volume 2: Nutrient Management and Crop Improvement (pp. 137-165). Springer Singapore.
Siregar P, Fauzi & Supriadi. 2017. Pengaruh Pemberian Beberapa Sumber Bahan Organik dan Masa Inkubasi Terhadap Beberapa Aspek Kimia Kesuburan Tanah Ultisol. Agroekoteknologi FP USU. 5(2): 256-264.
Triwulan Y. 2018. Pengaruh Kombinasi Sumber Fosfat dengan Bakteri Pelarut Fosfat terhadap Peningkatan Kadar P Tanah Alfisol dan Serapannya pada Tanaman Sorgum (Sorghum bicolor L. Moench). Universitas Jember.
Wijanarko A, Sudaryono & Sutarno. 2007. Karakteristik Sifat Kimia dan Fisika Tanah Alfisol di Jawa Timur dan Jawa Tengah. Iptek Tanaman Pangan. 2(2): 214-226.
Yama DI, Ivansyah O & Astriy R. 2021. Hubungan Serapan P dengan Pertumbuhan Setek Lada pada Aplikasi Kompos Ampas Tahu dan Jerami Padi. Agrotechnology Research. 5(2): 77-84.
Yelti SN, Zul D & Fibriarti BL. 2014. Formulasi Biofertilizer Cair Menggunakan Bakteri Pelarut Fosfat Indigenus Asal Tanah Gambut Riau. JOM FMIPA. 1(2): 651-662
Adiwijaya HD. 2019. Pengaruh Kombinasi Takaran Pupuk Nitrogen dan Pupuk Fosfat terhadap Pertumbuhan dan Hasil Tanaman Mentimun (Cucumis sativus L.) Kultivar Zatavy F1. Jurnal Agrorektan. 6(2): 2-21.
Alvernia P, Minardi S & Suntoro S. 2017. Zeolite and Organic Fertilizer Application to The Improvement of Available P and Soybean (Glycine max L) Seed Yield in Alfisols. Sains Tanah - Journal of Soil Science and Agroclimatology. 14(2): 84-90.
Alzate Zuluaga MY, Martinez de Oliveira AL, Valentinuzzi F, Tiziani R, Pii Y, Mimmo T, & Cesco S. 2021) Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants? Frontiers in Plant Science, 12:1-18.
Bertua, Irianto, & Ardiyaningsih. 2012. Pengaruh Dosis Pupuk Kandang Ayam terhadap Pertumbuhan dan Hasil Mentimun (Cucumis sativus L.) pada Tanah Ultisol. Bioplantae. 1(4): 266-273.
Cahyani P, Wijanarka & Raharjo B. 2017. Aktivitas Spesifik Selulase Serratia marcescens dengan Variasi Konsentrasi Amonium Sulfat ((NH4)2SO4) dan pH. Jurnal Biologi. 6(2): 41-49.
Cahyono O. 2018. Respon Beberapa Varietas Padi Sawah terhadap Cara Aplikasi Pupuk Fosfat di Tanah Alfisol. Jurnal Ilmiah Agrineca. 18(2): 22-33.
Campos CNS, Teixeira GCM, Prado RdeM, Caione G, da Silva Júnior GB, David CHO de, Sales AC, Roque CG & Teodoro PE. 2021. Macronutrient Deficiency in Cucumber Plants: Impacts in Nutrition, Growth and Symptoms. Journal of Plant Nutrition, 44(17): 2609-2626.
Chakraborty U, Chakraborty BN & Chakraborty AP. 2010. Influence of Serratia marcescens TRS-1 on Growth Promotion and Induction Of Resistance in Camellia Sinensis Against Fomes Lamaoensis. Journal of Plant Interactions. 5(4): 261-272.
Chen YP, Rekha PD, Arun AB, Shen FT, Lai W A & Young CC. 2006. Phosphate Solubilizing Bacteria from Subtropical Soil dnd Their Tricalcium Phosphate Solubilizing Abilities. Applied Soil Ecology. 34(1): 33-41.
Delfim J, Gerding M & Zagal E. 2020. Phosphorus fractions in Andisol and Ultisol inoculated with Bacillus thuringiensis and phosphorus uptake by wheat. Journal of Plant Nutrition. 43(18): 2728-2739.
Dhani H, Wardati & Rosmimi. 2014. Pengaruh Pupuk Vermikompos pada Tanah Inceptisol terhadap Pertumbuhan dan Hasil Sawi Hijau (Brassica juncea L.). JOM Faperta, 1(1): 1-11.
Fikdalillah, Basir M & Wahyudi I. 2016. Pengaruh Pemberian Pupuk Kandang Sapi terhadap Serapan Fosfor dan Hasil Tanaman Sawi Putih (Brassica pekinensis) pada Entisols Sidera. J. Agrotekbis. 4(5): 491-499.
Ginting EN, Pradiko I, Farrasati R & Rahutomo S. 2020. Pengaruh Rock Phosphate dan Dolomit terhadap Distribusi Perakaran Tanaman Kelapa Sawit pada Tanah Ultisols. Jurnal Agrikultura. 31(1): 32-41.
Hartatik W & Idris DK. 2008. Kelarutan Fosfat Alam dan SP-36 dalam Gambut yang Diberi Bahan Amelioran Tanah Mineral. Jurnal Tanah dan Iklim, 27: 45-56.
Kadarwati FT. 2016. Evaluasi Kesuburan Tanah untuk Pertanaman Tebu di Kabupaten Rembang, Jawa Tengah. Jurnal Penelitian Tanaman Industri. 22(2): 53-62.
Kannan P, Paramasivan M, Marimuthu S, Swaminathan C & Bose J. 2021. Applying Both Biochar and Phosphobacteria Enhances Vigna mungo L. Growth and Yield in Acid Soils by Increasing Soil pH, Moisture Content, Microbial Growth and P Availability. Agriculture, Ecosystems and Environment, 308: 1-9.
Kaur R & Kaur S. 2020. Variation in the Phosphate Solubilizing Bacteria from Virgin and the Agricultural Soils of Punjab. Current Microbiology. 77(9): 2118-2127.
Khan MA, Khatun A & Islam T. 2016. Promotion of Plant Growth by Phytohormone Producing Bacteria. In Industrial Microbiology: Microbes in Action (pp. 1-42).
Kurniawan AR, Bisri M & Suhartanto E. 2019. Analisis Kekeringan pada Daerah Aliran Sungai (DAS) Bedadung Berbasis Sistem Informasi Geografis (SIG). Jurnal Teknik Pengairan. 10(2): 97-109.
Ligowe IS, Young SD, Ander EL, Kabambe V, Chilimba ADC, Bailey EH, Lark RM & Nalivata PC. 2020. Agronomic biofortification of leafy vegetables grown in an Oxisol, Alfisol and Vertisol with isotopically labelled selenium (77Se). Geoderma. 361: 1-11.
Lovitna G, Nuraini Y & Istiqomah N. 2021. Pengaruh Aplikasi Bakteri Pelarut Fosfat dan Pupuk Anorganik Fosfat Terhadap Populasi Bakteri Pelarut Fosfat, P-Tersedia, dan Hasil Tanaman Jagung pada Alfisol. Jurnal Tanah Dan Sumberdaya Lahan. 8(2): 437-449.
Marra LM, Maria de Oliveira S, Roberto Fonsêca Sousa Soares C, Maria de Souza Moreira F & Borges Regitano J. 2011. Solubilisation of Inorganic Phosphates by Inoculant Strains From Tropical Legumes. Sci. Agric. 68(5): 603-609.
Marra LM, Sousa Soares CRF, de Oliveira SM, Ferreira PAA, Soares BL, de Carvalho RF, de Lima JM & de Moreira FMS. 2012. Biological Nitrogen Fixation and Phosphate Solubilization by Bacteria Isolated from Tropical Soils. Plant and Soil. 357(1): 289-307.
Mohamed EAH, Farag AG & Youssef SA. 2018. Phosphate Solubilization by Bacillus subtilis and Serratia marcescens Isolated from Tomato Plant Rhizosphere. Journal of Environmental Protection. 09(03): 266–277.
Mohammed WS, Mishra KS, Singh RK, Singh, MK & Soni SS. 2021. The effect of NPK on The Growth, Yield and Quality of Cucumber (Cucumis sativus L.) Under Protected cultivation. Journal of Pharmacognosy and Phytochemistry. 10(1): 2011-2014.
Nursyamsi D & Setyorini DD. 2009. Ketersediaan P Tanah-Tanah Netral dan Alkalin. Jurnal Tanah Dan Iklim, 30: 25-36.
Oksilia & Alby S. 2020. Pengaruh Pupuk Fosfat terhadap Pertumbuhan dan Produksi Mentimun Jepang (Cucumis sativus L.). Jurnal Ilmu Pertanian Agronitas. 2(2): 38-45.
Patel K, Patel S, Parekh V & Jha S. 2016. Isolation and Characterization of Salt Tolerant Phosphate Solubilizing Serratia marcescens Isolated from Coastal Area. Journal of Pure and Applied Microbiology, 10(3): 2401-2408.
Rawat P, Das S, Shankhdhar D & Shankhdhar, SC. 2021. Phosphate-Solubilizing Microorganisms: Mechanism and Their Role in Phosphate Solubilization and Uptake. Journal of Soil Science and Plant Nutrition. 21: 49-68.
Setyawan F. 2017. Pengaruh Bacillus subtilis dan Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Kedelai (Glycine max L.). Jurnal Hijau Cendekia. 2(1): 21-28.
Shirmohammadi E, Alikhani HA, Pourbabaei AA & Etesami H. 2020. Improved Phosphorus (P) Uptake and Yield of Rainfed Wheat Fed with P Fertilizer by Drought-Tolerant Phosphate-Solubilizing Fluorescent Pseudomonads Strains: a Field Study in Drylands. Journal of Soil Science and Plant Nutrition. 20(4): 2195-2211.
Shrivastava M, Srivastava PC & D’Souza SF. 2018. Phosphate-solubilizing microbes: Diversity and phosphates solubilization mechanism. In Role of Rhizospheric Microbes in Soil: Volume 2: Nutrient Management and Crop Improvement (pp. 137-165). Springer Singapore.
Siregar P, Fauzi & Supriadi. 2017. Pengaruh Pemberian Beberapa Sumber Bahan Organik dan Masa Inkubasi Terhadap Beberapa Aspek Kimia Kesuburan Tanah Ultisol. Agroekoteknologi FP USU. 5(2): 256-264.
Triwulan Y. 2018. Pengaruh Kombinasi Sumber Fosfat dengan Bakteri Pelarut Fosfat terhadap Peningkatan Kadar P Tanah Alfisol dan Serapannya pada Tanaman Sorgum (Sorghum bicolor L. Moench). Universitas Jember.
Wijanarko A, Sudaryono & Sutarno. 2007. Karakteristik Sifat Kimia dan Fisika Tanah Alfisol di Jawa Timur dan Jawa Tengah. Iptek Tanaman Pangan. 2(2): 214-226.
Yama DI, Ivansyah O & Astriy R. 2021. Hubungan Serapan P dengan Pertumbuhan Setek Lada pada Aplikasi Kompos Ampas Tahu dan Jerami Padi. Agrotechnology Research. 5(2): 77-84.
Yelti SN, Zul D & Fibriarti BL. 2014. Formulasi Biofertilizer Cair Menggunakan Bakteri Pelarut Fosfat Indigenus Asal Tanah Gambut Riau. JOM FMIPA. 1(2): 651-662
Published
2023-01-19
How to Cite
ABDILLAH, Muhammad Majid; SETIAWATI, Tri Candra.
The Utilization of Serratia marcescens to Increase Phosphate-Availability and Production of Cucumber in Alfisols.
Jurnal ILMU DASAR, [S.l.], v. 24, n. 1, p. 9-18, jan. 2023.
ISSN 2442-5613.
Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/29663>. Date accessed: 22 dec. 2024.
doi: https://doi.org/10.19184/jid.v24i1.29663.
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