Characterization of Protease from Endophytic Fungi Fusarium sp. JE-DP4a
Abstract
Protease is a catalytic enzyme that catalyzes a proteolysis process and breaks down a protein into smaller polypeptides or amino acids. Endophytic fungi can produce bioactive compounds, one of which is protease enzymes. The papaya leaf endophytic fungi used in this study was Fusarium sp. strain JE-DP4a. This study was conducted to determine the characterization of protease from endophytic fungi Fusarium sp. strain JE-DP4a with variations in incubation temperature of 30, 37, and 44oC and pH variation of 5.5-8. Measurement of protease enzyme activity using a spectrophotometer UV-Vis at 280 nm. Based on the research results, the highest protease activity produced at a temperature of 44oC is 53.78 U/mL, and specific activity is 17.31 U/mg protein. Protease activity at pH 7 shows the highest was 46.72 U/mL.Thus it is ably concluded that the protease produced by Fusarium sp. strain JE-DP4a belongs to the neutral protease group.
References
Agrawal PK, Rajput K & Chanyal S. 2016. Optimization of Protease Production by Endophytic Fungus, Alternaria alternata Isolate from gymnosperm tree-Cupressus torulosa D.Don.. World Journal of Pharmacy and Pharmaceutical Sciences. 5(7): 1034-1054.
Alnahdi HS. 2012. Isolation and Screening of Extracellular Proteases Produced by New Isolated Bacillus sp.. Journal of Applied Pharmaceutical Science. 2(9): 071-074.
Azarkan M, Dibiani R, Goormaghtigh E, Raussens V, & Bayens-Volant D. 2006. The Papaya Kunitz-Type Trypsin Inhibitor is A Highly Stable β-sheet glycoprotein. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomic. 1764: 1036-1072.
Chandrasekaran S, Kumaresan SSP & Manavalan M. 2015. Production and Optimization of Protease by Filamentous Fungus Isolated from Paddy Soil in Thiruvarur District Tamilnadu. Journal of Applied Biology & Biotechnology. 3(06): 066-069.
Choirunissa HN, Sari RY, Hastuti US & Witjoro A. 2017. Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah. Jurnal Bionature. 18(2): 99-109.
Chow BF & Peticolas M-A. 1948. A rapid Method for The Determination of Proteolytic Activities of Enzyme Preparations. Journal General of Physic, 32(1): 17-24.
Hashim SN, Ghazali SZ, Sidik NJ, Chia-Chay T & Saleh A. 2021. Surface Sterilization Method for Reducing Contamination of Clinacanthus Nutans Nodal Explants Intended for in-vitro culture. E3S Web of Conferences. 306: 01004.
Huang G, Tiejing Y, Po H & Jiaxing J. 2006. Purification and Characterization of A Protease From Thermophilic Bacillus strain HS08. African Journal of Biotechnology. 5(24): 2433–2438.
Huet J, Looze Y, Bartik K, Raussens V, Wintjens R & Boussard P. 2006. Structural Characterization of The Papaya Cysteine Proteinases at Low pH. Biochemical and Biophysical Research Communications. 341: 620-626.
Mahajan RT & Badgujar SB. 2010. Biological Aspects of Proteolytic Enzymes: A Review Purification of NGAL View Project Wound Healing Agents of Plant Origin. Journal of Pharmacy Research. 3(9): 2048-2068.
Muthulakshmi C, Gomathi D, Kumar DG, Ravikumar G, Kalaiselvi M & Uma C. 2011. Production, Purification and Characterization of Protease by Aspergillus Flavus Under Solid State Fermentation. Jordan Journal of Biological Sciences. 4(3): 137-148.
Nascimento AWC & Martins MLL. 2004. Production and Properties of An Extracellular Protease from Thermophilic Bacillus sp. Brazilian Journal of Microbiology. 35: 91-96.
Nirmala D, Yudha P, & Cahyanto D. 2021. The Effect Of Ph And Incubation Time on Rude Protease Enzymes Activity of Bacillus Mycoides from Anchovy Isolates (Stolephorus sp.). IOP Conference Series: Earth and Environmental Science. 679(1): 012075.
Noviyanti T, Ardiningsih P & Rahmalia W. 2012. Pengaruh Temperature Terhadap Aktivitas Enzim Protease dari Daun Sansakng (Pycnarrhena cauliflora Diels). Jurnal Kimia Khatulistiwa. 1(1): 31-34.
Ramadhani P, Rukmini MGI & Pujiyanto S. 2015. Produksi Enzim Protease dari Aspergillus niger PAM18A dengan variasi pH dan Waktu Inkubasi. Jurnal Biologi. 4(2): 25-34.
Rupali D. 2015. Screening and Isolation of Protease Producing Bacteria From Soil Collected from Different Areas of Burhanpur Region (MP) India. International Journal Current Microbiology and Apply Science. 4(8): 597-606.
Sharma AK, Sharma V, Saxena J, Yadav B, Alam A & Prakash A. 2015. Isolation and Screening of Extracellular Protease Enzyme from Bacterial And Fungal Isolates of Soil. International Journal of Scientific Research in Environmental Sciences. 3(9): 334-340.
Sonia S & Gupta S. 2015. Optimization of Protease Production from Fungi Isolated From Soil. International Journal of Applied Biology and Pharmaceutical Technology. 6(3): 149-153.
Souza PM, de Assis Bittencourt ML, Caprara C C, de Freitas M, de Almeida RPC, Silveira D, Fonseca YM, Filho EXF, Junior AP & Magalhães PO. 2015. A Biotechnology Perspective of Fungal Proteases. In Brazilian Journal of Microbiology. 46(2): 337-346.
Souza PM, Werneck G, Aliakbarian B, Siqueira F, Filho EXF, Perego P, Converti A, Magalhães PO & Junior AP. 2017. Production, Purification and Characterization of An Aspartic Protease from Aspergillus foetidus. Food and Chemical Toxicology. 109: 1103-1110.
Yusriah & Kuswytasari ND. 2013. Pengaruh pH dan Suhu Terhadap Aktivitas Protease Penicillium sp. Jurnal Sains dan Seni Pomits. 2(1): 48-50.
Zaferanloo B, Quang TD, Daumoo S, Ghorbani, MM, Mahon PJ & Palombo EA. 2014. Optimization of Protease Production by Endophytic Fungus, Alternaria Alternata, Isolated from an Australian native plant. World Journal of Microbiology and Biotechnology. 30(6): 1755-1762.
Alnahdi HS. 2012. Isolation and Screening of Extracellular Proteases Produced by New Isolated Bacillus sp.. Journal of Applied Pharmaceutical Science. 2(9): 071-074.
Azarkan M, Dibiani R, Goormaghtigh E, Raussens V, & Bayens-Volant D. 2006. The Papaya Kunitz-Type Trypsin Inhibitor is A Highly Stable β-sheet glycoprotein. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomic. 1764: 1036-1072.
Chandrasekaran S, Kumaresan SSP & Manavalan M. 2015. Production and Optimization of Protease by Filamentous Fungus Isolated from Paddy Soil in Thiruvarur District Tamilnadu. Journal of Applied Biology & Biotechnology. 3(06): 066-069.
Choirunissa HN, Sari RY, Hastuti US & Witjoro A. 2017. Identifikasi dan Uji Kemampuan Hidrolisis pada Bakteri Amilolitik dan Proteolitik yang Diisolasi dari Wadi, Makanan Khas Kalimantan Tengah. Jurnal Bionature. 18(2): 99-109.
Chow BF & Peticolas M-A. 1948. A rapid Method for The Determination of Proteolytic Activities of Enzyme Preparations. Journal General of Physic, 32(1): 17-24.
Hashim SN, Ghazali SZ, Sidik NJ, Chia-Chay T & Saleh A. 2021. Surface Sterilization Method for Reducing Contamination of Clinacanthus Nutans Nodal Explants Intended for in-vitro culture. E3S Web of Conferences. 306: 01004.
Huang G, Tiejing Y, Po H & Jiaxing J. 2006. Purification and Characterization of A Protease From Thermophilic Bacillus strain HS08. African Journal of Biotechnology. 5(24): 2433–2438.
Huet J, Looze Y, Bartik K, Raussens V, Wintjens R & Boussard P. 2006. Structural Characterization of The Papaya Cysteine Proteinases at Low pH. Biochemical and Biophysical Research Communications. 341: 620-626.
Mahajan RT & Badgujar SB. 2010. Biological Aspects of Proteolytic Enzymes: A Review Purification of NGAL View Project Wound Healing Agents of Plant Origin. Journal of Pharmacy Research. 3(9): 2048-2068.
Muthulakshmi C, Gomathi D, Kumar DG, Ravikumar G, Kalaiselvi M & Uma C. 2011. Production, Purification and Characterization of Protease by Aspergillus Flavus Under Solid State Fermentation. Jordan Journal of Biological Sciences. 4(3): 137-148.
Nascimento AWC & Martins MLL. 2004. Production and Properties of An Extracellular Protease from Thermophilic Bacillus sp. Brazilian Journal of Microbiology. 35: 91-96.
Nirmala D, Yudha P, & Cahyanto D. 2021. The Effect Of Ph And Incubation Time on Rude Protease Enzymes Activity of Bacillus Mycoides from Anchovy Isolates (Stolephorus sp.). IOP Conference Series: Earth and Environmental Science. 679(1): 012075.
Noviyanti T, Ardiningsih P & Rahmalia W. 2012. Pengaruh Temperature Terhadap Aktivitas Enzim Protease dari Daun Sansakng (Pycnarrhena cauliflora Diels). Jurnal Kimia Khatulistiwa. 1(1): 31-34.
Ramadhani P, Rukmini MGI & Pujiyanto S. 2015. Produksi Enzim Protease dari Aspergillus niger PAM18A dengan variasi pH dan Waktu Inkubasi. Jurnal Biologi. 4(2): 25-34.
Rupali D. 2015. Screening and Isolation of Protease Producing Bacteria From Soil Collected from Different Areas of Burhanpur Region (MP) India. International Journal Current Microbiology and Apply Science. 4(8): 597-606.
Sharma AK, Sharma V, Saxena J, Yadav B, Alam A & Prakash A. 2015. Isolation and Screening of Extracellular Protease Enzyme from Bacterial And Fungal Isolates of Soil. International Journal of Scientific Research in Environmental Sciences. 3(9): 334-340.
Sonia S & Gupta S. 2015. Optimization of Protease Production from Fungi Isolated From Soil. International Journal of Applied Biology and Pharmaceutical Technology. 6(3): 149-153.
Souza PM, de Assis Bittencourt ML, Caprara C C, de Freitas M, de Almeida RPC, Silveira D, Fonseca YM, Filho EXF, Junior AP & Magalhães PO. 2015. A Biotechnology Perspective of Fungal Proteases. In Brazilian Journal of Microbiology. 46(2): 337-346.
Souza PM, Werneck G, Aliakbarian B, Siqueira F, Filho EXF, Perego P, Converti A, Magalhães PO & Junior AP. 2017. Production, Purification and Characterization of An Aspartic Protease from Aspergillus foetidus. Food and Chemical Toxicology. 109: 1103-1110.
Yusriah & Kuswytasari ND. 2013. Pengaruh pH dan Suhu Terhadap Aktivitas Protease Penicillium sp. Jurnal Sains dan Seni Pomits. 2(1): 48-50.
Zaferanloo B, Quang TD, Daumoo S, Ghorbani, MM, Mahon PJ & Palombo EA. 2014. Optimization of Protease Production by Endophytic Fungus, Alternaria Alternata, Isolated from an Australian native plant. World Journal of Microbiology and Biotechnology. 30(6): 1755-1762.
Published
2023-07-06
How to Cite
HASTUTY, Aerma; HERAWATI, Irma; PIKOLI, Megga Ratnasari.
Characterization of Protease from Endophytic Fungi Fusarium sp. JE-DP4a.
Jurnal ILMU DASAR, [S.l.], v. 24, n. 2, p. 129-134, july 2023.
ISSN 2442-5613.
Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/27293>. Date accessed: 29 dec. 2024.
doi: https://doi.org/10.19184/jid.v24i2.27293.
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