Antagonism Potentials of Endophytic Fungi from Lemongrass (Cymbopogon nardus (L.) Rendle) on Escherichia coli
Abstract
This study aimed to determine the potential antagonism performed by the endophytic fungi of lemongrass against Escherichia coli. Five species of endophytic fungi were tested using agar well method. These fungi consist of Paecilomyces lilacinus, Penicillium sp., Trichoderma viride, Fusarium oxysporum, and Fusarium sp., each was taken from roots, stem, and leaves, respectively. Chloramphenicol and aquadest were used as control. The results showed that there is significant inhibition of E. coli compared to the negative control. Paecilomyces lilacinus showing the most prominent inhibition with 74.67%, followed by unknown species of Fusarium 47.33%, Penicillium sp 42.67%, Fusarium oxysporum 21.67%, and Trichoderma viride 17.43%. It can be concluded that all five isolates of endophytic fungi from lemongrass (Cymbophogon nardus (L.) Rendle) have the ability to inhibit the growth of E. coli, and thus have antagonism potential against E. coli.
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Astawan M,Wresdiyati T, Arief I & E. Suhesti. 2011. Gambaran Hematologi Tikus Putih (Rattus norvegicus) yang Diinfeksi Escherichia coli Enteropatogenik dan Diberikan Probiotik. Media Peternakan. 34(1): 7-13.
Ayuningsih TW. 2020. Eksplorasi Fungi Endofit pada Rhizoma dan Daun Tanaman Serai Merah (Cymbopogon nardus L. Rendle) serta Pemanfaatannya sebagai Buku Ilmiah Populer. Skripsi yang tidak dipublikasi pada Universitas Jember.
Bota W, Martosupomo M & Rondonuwo FS. 2015. Potensi Senyawa Minyak Sereh Wangi (Citronella oil) dari Tumbuhan Cymbopogon nardus L. sebagai Agen Antibakteri. Prosiding Seminar Nasional Sains dan Teknologi. 17 November 2015. Fakultas Teknik Universitas Muhammadiyah Jakarta: 1-8.
Carolia, N. dan W. Noventi. 2016. Potensi Ekstrak Daun Sirih Hijau (Piper bettle L.) sebagai Alternatif Terapi Acne vulgaris. Majority. 5(1): 140-145.
Cushnie TPT, Cushnie B & Lamb AJ. 2014. Alkaloids: an Overview of Their Antibacterial, Antibiotic-Enhancing and Antivirulence Activities. International Journal of Antimicrobial Agents. 44: 377-386.
Dai, Ze-Bao. Xin Wang & Guo-Hong Li. 2020. Secondary Metabolites and Their Bioactivities Produces by Paecilomyces. The Molecules. 25: 1-18.
Dewi ZY, Nur A & Hertriani T. 2015. Efek Penghambatan Biofilm Ekstrak Sereh (Cymbopogon nardus L.) terhadap Bakteri Streptococcus mutans. Majalah Kedokteran Gigi Indonesia. 1(2): 136-141.
Dhifi WS, Bellili, Jazi S, Bahloul N & Mnif W. 2016. Essential Oils’ Chemical Characterization and Investigation of Some Biological Activities: A Critical review. The Medicine (Basel). 3(4):25.
Jawetz, Melnick & Adelberg. 2004. Mikrobiologi Kedokteran. Edisi 23. Jakarta: Penerbit Buku Kedokteran EGC.
Kaul S, Gupta S, Ahmed M & Dhar MK. 2012. Endophytic Fungi from Medicinal Plant: a Treasure Hunt for Bioactive Metabolites. Phytochemistry Reviews. 11: 487-505.
Kurniati A & Ali M. 2018. Isolasi dan Uji Antagonis Jamur Asal Rizosfer Tanaman Bawang Merah (Allium ascalonicum L.) terhadap Alternania porri Ellis Cif. JOM Pertanian. 5: 1- 10.
Ledwaba SE, Costa D, Bolick DT, Giallourou N, Medeiros P, Swann JR, Traore AN, Potgieter N, Nataro JP & Guerrant RL. 2020. Enteropathogenic Escherichia coli Infection Induces Diarrhea, Intestinal Damage, Metabolic Alterations, and Increased Intestinal Permeability in a Murine Model. Frontiers in cellular and infection microbiology, 10:595266.
Lien LTQ, Lan PT, Chuc NTK, Hoa NQ, Nhung PH, Thoa NTM, Diwan V, Tamhankar AJ & Lundborg CS. 2017. Antibiotic Resistance and Antibiotic Resistance Genes in Escherichia coli Isolates from Hospital Wastewater in Vietnam. Int. Journal of Environment Re. Public Health. 14(7): 1-11.
Maier, RM. 2009. Bacterial Growth. Book Chapter dalam Review of Basic Microbial Concepts. Academic Press. Inc.
Niasono AB, Latif H & Purnawarman T. 2019. Resistensi Antibiotik terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat. Jurnal Veteriner. 20(2): 187-195.
Nord C, Levenfors JJ, Bjerketorp J, Sahlberg. C, Guss B, Oberg B & Broberg A. 2019. Antibacterial Isoquinoline Alkaloids from The Fungus Penicillium spathulatum Em19. The Molecules. 24: 1-14.
Pu, Zhong-Hui, Zhang YQ, Yin ZQ, Xu J, Jia RY, Lu Y, Yang F. 2010. Antibacterial Activity of 9-Octadecanoic Acid-Hexadecanoic Acid-tetrahydrofuran-3,4-Diyl Ester from Neem Oil. Agricultural In China. 9(8): 1236-1240.
Puspawati NM, Suirta IW & Bahri S. 2016. Isolasi, Identifikasi, serta Uji Aktivitas Antibakteri pada Minyak Atsiri Sereh Wangi (Cymbopogon winterianus Jowitt). Jurnal Kimia. 10(2): 219-227.
Qing ZX, Yang P, Tang Q, Cheng P, Liu XB, Zheng YJ, Liu YS & Zeng JG. 2017. Isoquinoline Alkaloids and Their Antiviral, Antibacterial, and Antifungal Activities and Structure-Activity Relationship. Current Organic Chemistry. 21: 1-15.
Rianto A, Isrul M, Anggarini S & A. Saleh. 2018. Isolasi dan Identifikasi Fungi Endofit Daun Jambu Mete (Anacardium occidentale L.) sebagai Antibakteri terhadap Salmonella typhimurium. Jurnal Mandala Pharmacon Indonesia. 4(2): 109-121.
Ruswandari VR, Syauqi A & Rahayu T. 2020 Uji Antagonis Jamur Trichoderma viride dalam Menghambat Pertumbuhan Jamur Patogen Alternaria porri Penyebab Penyakit Bercak Ungu pada Tanaman Bawang Merah (Allium ascalonicum L.). Jurnal Ilmiah Biosainstropis. 5(2): 84-90.
Sanders, ER. 2012. Aseptic Laboratory Technique: Plating Methods. Journal of Visualized Experiments. 63: 1-18.
Sanjaya Y, Nurhaeni H & Halima M. 2010. Isolasi, Identifikasi, dan Karakterisasi Jamur Entomopatogen dari Larva Spodoptera litura (Fabricus). Bionatura. 12(3): 136-141.
Sarlina S, Razak AR & Tandah MR. 2017. Uji Aktivitas Antibakteri Sediaan Gel Ekstrak Daun Sereh (Cymbopogon nardus L. Rendle) terhadap Bakteri Staphylococcus aureus Penyebab Jerawat. Jurnal Farmasi Galenika. 3(2): 143-149.
Venieraki A, Dimou M & Katinakis P. 2017. Endophytic Fungi Residing in Medicinal Plants have The Ability to Produce the Same or Similar Pharmacologically Active Secondary Metabolites as Their Hosts. Hellenic Plant Protection Journal. 10:51-66.
Watanabe S, Ohbayashi R, Kanesaki Y, Saito N, Chibazakura T, Soga T, et al. 2015 Intensive DNA Replication and Metabolism During The Lag Phase in Cyanobacteria. PLoS ONE 10(9): e0136800.
Yulia E, Istifadah N, Widiantini F & Utami HS. 2017. Antagonisme Trichoderma spp. terhadap Jamur Rigidoporus lignosus (Klotzsch) Imazeki dan Penekanan Penyakit Jamur Akar Putih pada Tanaman Karet. Jurnal Agrikultura. 28(1): 47-55.
Zamzani, M.C. 2011. Aktivitas Antibakteri Ekstrak Etanol Tanaman Serai (Cymbopogon nardus (L.) Rendle) terhadap Staphylococcus aureus dan Escherichia coli Multiresisten serta Bioautografinya. Thesis. Universitas Muhammadiyah Surakarta.
Published
2022-01-14
How to Cite
AL HADI, Salma Khatami; NARULITA, Erlia; MURDIYAH, Siti.
Antagonism Potentials of Endophytic Fungi from Lemongrass (Cymbopogon nardus (L.) Rendle) on Escherichia coli.
Jurnal ILMU DASAR, [S.l.], v. 23, n. 1, p. 49-54, jan. 2022.
ISSN 2442-5613.
Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/21390>. Date accessed: 27 apr. 2024.
doi: https://doi.org/10.19184/jid.v23i1.21390.
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