Response of Entomopathogenic Fungus Paecilomyces fumosoroseus on Drought Stress Factor
Abstract
The entomopathogenic fungus Paecilomyces fumosoroseus (common name: Isaria fumosorosea) can utilize to control white fly population. Bemisia tabaci or white fly has become key pest in soybean cultivation. It reported became resistance due to chemical pesticide. Some of new strain has been emerge as chemical pesticide resultant However, to develop P. fumosoroseus as biopesticide hide a problem. The environmental drought factor (temperature and water stress) become major problem. This research aimed to determine effect of environmental factor like temperature and water stress to growth and effectivity of P. fumosoroseus, due to selection an isolat were persist to drought factor. In this research has been used two different isolat of P. fumosoroseus, that is Wirowongso 1 isolat and Mumbulsari 5 isolates. This research had been conducted with five different treatments. First is a growth test under temperature stress, in vitro germination test under temperature stress and in vitro germination test under water stress, and virulence test under temperature and under water stress. The result showed that the increase a temperature and of water stress, directly make decreased of growth, germination and effectively. That effect has made different effect to growth, germination and effectivity on both isolat. The results showed that the isolat WR 1 test result showed the isolat WR 1 more persist to each drought factor. It became more valuable to develop as biopesticide among other.
Keywords: Bemicia tabaci, germination, isolat, Isaria fumosorosea, in-vivo.
References
Castrillo LA, Griggs MH & Vandenberg JD. 2004. Vegetative Compatibility Groups in Indigenous and Mass-released Strains of The Entomopathogenic Fungus Beauveria bassiana: Likelihood of Recombination in The Field. Journal of Invertebrate Pathology. 86(1-2): 26-37.
Devi KU, Sridevi V, Mohan Ch.M & Padmavathi J. 2005. Effect of High Temperature and Water Stress on in vitro Germination and Growth in Isolats of The Entomopathogenic Fungus Beauveria bassiana (Bals.) Vuillemin. Journal of Invertebrate Pathology. 88(3):181-189.
Flexner JL & Belnavis DL. 1999. Microbial Insecticides. Biological and Biotechnological Control of Insect Pests. CRC Press: 35-62.
Gaur AC. Sadasivan KV, Mathur RS & Magu SP. 1982. Role of mesophilic fungi in composting. Agricultural Wastes. Elsevier, 4(6):453-460.
Gleason FH, Midgley DJ, Letcher PM & Mcgee PA. 2006. Can soil Chytridiomycota survive and grow in different osmotic potentials? Mycological Research. 110(7):869-875.
Hadi YS & Yusuf S. 2000. Keefektifan Beberapa Spesies Cendawan Entomopatogen untuk Mengendalikan Rayap Tanah Coptotermes gestroi WASMANN (Isoptera : Rhinotermitidae) dengan Metode Kontak dan Umpan Effectiveness of Some Entomopathogenic Fungi Species as Bio-control Agent to Subt. Bio-Control: 5(2).
Jackson SL & Bayliss KL. 2011 Spore Traps Need Improvement to Fulfil Plant Biosecurity Requirements. Plant Physiology. 60(5): 801-810.
Jessica JJ, Peng TL, Sajap AP, Lee SH & Syazwan SA.. 2019. Evaluation of The Virulence of Entomopathogenic Fungus, Isaria fumosorosea Isolates against Subterranean Termites Coptotermes spp. (Isoptera: Rhinotermitidae). Journal of Forestry Research. Springer. 30(1):213-218.
Kalkar, Ö. Carner GR, Scarf D & Boucias DG 2006. Characterization of an Indonesian Isolat of Paecilomyces reniformis. Mycopathologia.161(2): 109-118.
Kassa A. 2003. Development and Testing of Mycoinsecticides Based on Submerged Spores and Aerial Conidia of The Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) for control of locusts, grasshoppers and storage pests. Doctoral Thesis. Georg-August-Universitat Gottingen. Germany
Lozano-Contretas MG, Maldonado-Blanco MG, Elias-Santos M, Gonzales-Hernandez A & Nava-Camberos U. 2013. Application of Isaria fumosorosea Blastospores Produced in Liquid Culture for Control of Bemisia argentifolii on Cotton Plants. Southwestern Entomologist. 38(1): 57-66.
Purnomo H. 2010. Pengantar Pengendalian Hayati. Penerbit Andi.
Sandoval-Coronado CF, Luna-Olvera HA, Arévalo-Niño K, Jackson MA, Poprawski TJ & Galán-Wong LJ. 2001. Drying and Formulation of Blastospores of Paecilomyces fumosoroseus (Hyphomycetes) Produced in Two Different Liquid Media. World Journal of Microbiology and Biotechnology. 17(4): 423-428.
Smith PE. 2009. Whitefly: identification and biology in New Zealand greenhouse tomato crops. Sustainable Farming Fund. Factsheet.1:1-8.
Sun J, Fuxa JR & Henderson G. 2003. Effects of Virulence, Sporulation, and Temperature on Metarhizium anisopliae and Beauveria bassiana Laboratory Transmission in Coptotermes formosanus. Journal of Invertebrate Pathology. 84(1): 38-46.
Varela A & Morales E. 1996. Characterization of Some Beauveria bassiana Isolats and Their Virulence toward The Coffee Berry Borer Hypothenemus hampei. Journal of Invertebrate Pathology. 67(2): 147-152.
Vidal C, Fargues J, Lacey LA & Jackson MA 1998. Effect of Various Liquid Culture Media on Morphology, Growth, Propagule Production, and Pathogenic Activity to Bemisia argentifolii of The Entomopathogenic Hyphomycete, Paecilomyces fumosoroseus. Mycopathologia. 143(1):33-46.
Winder RS. Wheeler JJ, Coner N, Otvos IS, Nevill R & Duan L. 2003. Microencapsulation: a Strategy for Formulation of Inoculum. Biocontrol Science and Technology. 13(2): 155-169.
Zhang H, Tang M. & Yang Y. 2011. The Response of Ectomycorrhizal (ECM) Fungi under Water Stress Induced by Polyethylene Glycol (PEG) 6000. African Journal of Microbiology Research. Academic Journals 5(4): 365-373.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.