Induction Somatic Embryogenesis Used 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Kinetin in Spindle Leaf Explant Sugarcane

  • Wardatus Sholeha Jember University
  • Bambang Sugiharto Jurusan Biologi, FMIPA, Universitas Jember Center for Development of Advanced Science and Technology (CDAST), Universitas Jember
  • Dwi Setyati Jurusan Biologi, FMIPA, Universitas Jember
  • Parawita Dewanti Jurusan Agronomi, Fakultas Pertanian, Universitas Jember

Abstract

Induction of somatic embryogenesis in sugarcane requires the composition Plant Growth Hormone (PGH) appropriate. Utilizing of PGH (2,4-D and kinetin) is expected to induce sugarcane somatic embryogenesis. The purpose of this study was to obtain the concentration of 2,4-D and kinetin that effective for the multiplication of sugarcane var. NXI 1-3 through somatic embryogenesis. This study consists of four stages: callus induction, callus proliferation, regeneration of shoots and encapsulation. The plant material used is a spindle leaf sugarcane var. NXI 1-3. Callus induction used 2,4-D with different concentration (2 ppm, 3 ppm and 4 ppm). Callus proliferation used 2,4-D with concentration 1 ppm and 2 ppm. Regeneration of shoots used kinetin 0,5 ppm. The results are showed that the optimal induction of embryogenic callus on medium MS + sucrose 30 g / L + CH 300 ppm + 300 ppm PVP + 2,4-D 4 ppm as indicated by the high percentage of explants forming embryogenic callus that is equal to 40% and the respective concentration 2 ppm and 3 ppm is 33,3% and 37,5%. In proliferation stage, the development callus optimal on medium MS + sucrose 30 g / L + CH 300pm + PVP 300 ppm + 2,4-D 2 ppm and formulations for regeneration shoot on medium MS + sucrose 30 g / L + kinetin 0.5 ppm. The result of encapsulation can be shaped 100 sythetic seed.

Keywords: Somatic embryogenesis, spindle leaf, kinetin, 2,4-D
Published
2015-08-14
How to Cite
SHOLEHA, Wardatus et al. Induction Somatic Embryogenesis Used 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Kinetin in Spindle Leaf Explant Sugarcane. Jurnal ILMU DASAR, [S.l.], v. 16, n. 1, p. 17-22, aug. 2015. ISSN 2442-5613. Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/1387>. Date accessed: 02 june 2020. doi: https://doi.org/10.19184/jid.v16i1.1387.
Section
General