SCREENING AND PARTIAL CHARACTERIZATION OF BACTERIOCIN FROM LACTID ACID BACTERIA ISOLATED FROM FAN PALM SUGAR (Borassus flabellifer L)

Abstract

Bacteriocin is a protein compound that has bactericidal action against microorganism.
Bacteriocins from lactid acid bacteria are very potensial as natural food biopreservatives.
The aim of this present study is to obtain the isolate of lactic acid bacteria which has a
potential to produce bacteriocin from fan palm sugar, to attain the bacteriocin
characterization such as its stability against heat and proteolytic enzyme. Other goal is to
observed the inhibitory activity of bacteriocin against Gram positive and Gram negative
bacteria. This research found that 2 isolates LB.9 and LB.30 have a potency to produce
bacteriocin. LB.9 sensitive to protease enzyme and heat labile whereas isolate LB.30
sensitive to protease enzyme and heat stable. The bacteriocin are able to inhibit the growth
of Gram positive and Gram negative bacteria.