Analisis Profil Protein Ekstrak Aquades dan Etanol Daun Mimba (Azadirachta Indica A. Juss) dengan Metode SDS-PAGE (Protein Profile Analysis of Aquadest and Ethanol Extract of Neem Leaves by Means of SDS-PAGE Method)

Abstract

Background: Neem leaf is used to increase body imune response, antivirus, antifungi,
antibacterial, dan antioxidant. These biology activities was suspected because of protein
in neem leaf. Protein in plants can be used as pharmacogenetic identification because
some proteins in plants posse characteristic can defense to some pathogen
mcroorganism. The way to know protein in the organism is using profile protein analytic
through SDS-PAGE method. SDS-PAGE separates protein molecules by size and
particular shapping and also can determine protein molecular weight. Protein profiles
can be obtained with protein extracts using solvents. Protein is a polar compound which
is bonded to a polar solvent therefore chosen ethanol and aquadest as a liquid as
extraction solvent . Purpose: To know the protein profile of aquadest and ethanol
extracts of neem leaf by using SDS-PAGE. Method: This study uses neem leaf as a
sample, aquadest and 70% ethanol as the extraction solvent then analyzed using SDSPAGE.
Result: The results showed 9 protein bands on the aquadest extract of neem
leaf and 8 of protein bands on the ethanol extract of neem leaf. Conclusion: From the
study, there is a difference between the amount of protein bands aquadest extracts of
neem leaf with ethanol extract of neem leaf.
Keywords: aquadest extract, ethanol extract, neem leaf, protein profile, SDS-PAGE