Ekspresi BMP2 dan TRAF6 pada Jaringan Periodontal  setelah Pemberian Ekstrak Kopi Robusta  selama Pergerakan Gigi Ortodonti

Herniyati Herniyati; Leliana Sabdra deviade Putri; Hestieyonini Hadnyanawati; Happy Harmono

doi:10.19184/pk.v11i2.38029

Herniyati Herniyati Fakultas Kedokteran Gigi Universitas Jember
Leliana Sabdra deviade Putri Fakultas Kedokteran Gigi Universitas Jember
Hestieyonini Hadnyanawati Fakultas Kedokteran Gigi Universitas Jember
Happy Harmono Fakultas Kedokteran Gigi Universitas Jember

DOI: https://doi.org/10.19184/pk.v11i2.38029

Abstract

Orthodontic tooth movement occurs due to mechanical stimulation followed by alveolar bone and periodontal ligament remodeling. Bone remodeling is the process of bone resorption in areas of tension and bone formation in areas of tension. Various techniques and drugs are used to speed up orthodontic treatment. This study aims to analyze the expression of BMP2 and TRAP6 on orthodontic tooth movement after the administration of Robusta coffee extract. Twenty-four Wistar rats were divided into 4 groups: C1 (2 weeks) and C2 (3 weeks) groups: rats were given orthodontic mechanical force (OMF) and T1 (2 weeks) and T2 (3 weeks) groups rats were given OMF and Robusta coffee extract. OMF in rats was performed by moving the maxillary right permanent molars mesially with a Niti closed coil spring. Observations were made on the 15th and 22nd day by immunohistochemical examination to determine the expression of BMP2 and TRAP6. Administration of Robusta coffee extract for 2 weeks and 3 weeks increased BMP2 and TRAP6 expression (p <0.05). Administration of Robusta coffee extract increased the expression of BMP2 and TRAP6 in the alveolar bone during orthodontic tooth movement.

References

[1] Nimeri G, Kau CH, Aboe-kheir NS, Corona R,. Acceleration of tooth movement during orthodonic treatment – a frontier in Orthodontic. Prog Orthod. 2013; (14): 14-42.
[2] Te H, Arron JR, Lamothe B, Cirilli M, et
al. 2002. Nature. 2002; 418( 6896), 443-447.
[3] Bai S, Kitaura H, Zhao H, Chen J, Muller JM, Schule R, Darnay B, novac DV, FP, Teitelbaum SL. FHL2 inhibits the activated osteoclast in a TRAF6-dependent manner. J Clin Invest. 2005; 115(10): 2742-2751
[4]. Lamoyhe B, William K, Webster WK, Ambily K , Webster AK, Gopinathan A , Besse A, D Campos A, Bryant G Darnay BG. TRAF6 U biquitin Ligase is Essential for RANKL Signaling and Osteoclast Differentiation. Biochemical and biophysical research communications. 2007; 359(4),1044-49,
[5] Mundy GR, Chen D, and Oyajobi BO. Bone Remodeling, in Prime on the Metabolic Diseases and Disorder of Mineral Metabolism. Favus MJ ed, Washington DC: American Society of Bone and Mineral Research, 2003.
[6] Shenava S, Nayak KUS, Bhaskar V, Nayak A. Accelerated Orthodontics-A Review. IJSRP Journal. 2014; 1(5): 35-39
[7] Sukendro S. Keajaiban Dalam Secangkir Kop. Penerbit: Media Pressindo Yogyakarta; 2013.
[8] Yashin, A., Y. Yashin, J.Y. Wang, dan B.S. Nemzer. Antioxidant and Antiradical Activity of Coffee. Antioxidants. 2013; 11(2): 230-245.
[9] Richelle MI, Tavazzi, Offord E.l. Comparison of the antioxidant of commonly consumed Polyphenole Beverage (Coffee, Cocoa, Tea) Prepared per cup serving. Journal of Agricultural and Food Chemistry. 2001; 49(70): 3438-3444
[10] Galicka A, Nazaruk J. Stimulation of Collagen Biosynthesis by Flavonoid Glycosides in Skin Fibroblasts of Osteogenesis Imperfecta Type 1 and the Potential Mechanism of their action. IJM Medicine. 2007; ( 20): 889-895.
[11] Herniyati, Putri LSD, Wijayanti RPA. Effects of Robusta coffee extract on Increasing fibroblast number and Density of Collagen Fibers of Periodontal Ligament in Orthodontic Tooth Movement. Forkinas 2019. Aston Hotel 6-7 September 2019.
[12] Akhoundi MSA, Mehr MS , Keshvad MA, Moghaddam SE , Alaeddini M , Dehpour A , Mirhashemi AH Effect of amitriptyline on orthodontic tooth movement in rats: an experimental study. J Dent Res Dent Clin Dent Prospects 2020; 14(3): 147-152
[13] Kim MK,and Niyibizi C. Interaction of TGFβ-1 and rhBMP-2 on human bone marrow stromal cells cultured in collagen gel matrix. Yonsei Med J 2001.:42(3): 338-344.
[14] Thailander B, Rygh P, and Reitan K. Tissue Reaction In Orthodontics. In Orthodontics Current Principles And Techniques. 3rd ed., St. Louis : Mosby; 2000.
[15] Cao X and Chen D. The BMP Signalling and in Vivo Bone Formation. Gene. , 2005.: (357): 1-8.
[16] Day TF, Guo X, Garrett- Beal L and Yang Y. Wnt/beta Catenin Signalling in Mesenchymal Progenitors Controls Osteoblast and Chondrocyte Differentiation during Vertebrate Skeletogenesis, Dev. Cell. , 2005: (8): 739-750.
[17] Ducy P, Zhang R, Geooffroy V, Ridall AL, and Karsenty G.. Osf2/Cbfa1: a Transcriptional Activator of Osteoblast Differentiation. Cell. 1997: (89): 747-754.
[18] Crockett JC, Rogers MJ, Coxon FP, Hocking LJ, and Helfrich MH. Bone emodelling at a Glance, J Cell. 2011; (124): 991-998.
[19] Mamun MA, Islam K, Alam MJ, Khatun A, Alam MM, Al-Bari MA, Alam MJ. Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption. Biol Res. 2015; (48): 51.
[20] Mitra C, Das D, Das AS, Preedy VR. Black tea (Camellia sinensis) and bone loss protection. In: Preedy VR, editor. Tea in health and disease prevention. London: Academic Press; 2013.
[21] Shen C-L, Yeh JK, Cao JJ, Chyu M-C, Wang J-S. Green tea and bone health: 22. Mamun MA, Islam K, Alam MJ, Khatun A, Alam MM, Al-Bari MA, Alam MJ. Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption. Biol Res. 2015; (48): 51.
[22] Nash LA, Sullivan PJ, Peters SJ, Ward WE. Rooibos flavonoids, orientin and luteolin, stimulate mineralization in human osteoblasts through the Wnt pathway. Mol Nutr Food Res. 2015; (59): 443-53.
[23] Haq MJ, Riaz M, Feo VD, Jaafar HZE. Moga M . Rubus fruticosus L.: constituents, biological activities and health related uses. Molecules. 2014; 19(8):10998-1029.
[24] Crockett JC, Rogers MJ, Coxon FP, Hocking LJ, and Helfrich MH. Bone Remodelling at a Glance, J Cell. 2011; (124): 991-998.
[25] Jianru Y, Boxi Y, Meile L, Yu W, Wei Z, Yu L, and Zhihe Z. Caffeine may enhance orthodontic tooth movement through increasing osteoclastogenesis induced by periodontal ligament cells under compression. Archives of Oral Biology. 2016 ( 64): 51-60.
[26] Herniyati. Pengaruh Kafein Terhadap Ekspresi RANKL dan Jumlah Osteoklas Pada Pergerakan Gigi Ortodonti. Denta Jurnal Kedokteran Gigi. 2016; 10(1): 62-70
[27] Choi J, Choi SY, Lee SY, Lee JY, Kim HS, Lee SY, Lee NK. Caffeine enhances osteoclast differentiation and maturation through p38 MAP kinase/Mitf and DC-STAMP/CtsK and TRAP pathway. Cell Signal. 2013; 25(5): 1222-7.
[28] Liu XH et al. Interactive effect of interleukin-6 and prostaglandin E2 on osteoclastogenesis via the OPG/RANKL/RANK system. Ann N Y Acad Sci. 2006; (1068): 225-33.
[29] Boyce, Brendan F.MD, and Lianping X.MD. Functions of RANKL/RANK/OPG in bone modeling and remodeling. Arch Biochem Biophys. 2007; 473(2):139-146.
[30] Zhao Q, Wang Xi, Liu Y, He Aimin, Jia Ruokun. NFATc1: Functions in osteoclasts, iJBCB. 2010; 42(5): 579- 576.