Uji Aktivitas Antibakteri Ekstrak Etanol Daun Waru Gunung (Hibiscus macrophyllus) dan Fraksinya terhadap Staphylococcus aureus

  • Afif Rifqie Maulana Fakultas Farmasi Universitas Jember
  • Bawon Triatmoko Fakultas Farmasi Universitas Jember
  • Mochammad Amrun Hidayat Fakultas Farmasi Universitas Jember

Abstract

Infection is one of the main causes of world health problems, especially in developing countries like Indonesia, one of which is an acute respiratory infection caused by bacteria such as Staphylococcus aureus. Therapeutics are used for the treatment of infections today with antibiotics. However, there are many cases of bacterial resistance to antibiotics that need to develop alternatives derived from plants. One of the plants that have antibacterial activity is the leaves of Waru Gunung (Hibiscus macrophyllus). The purpose of this study was to determine whether the ethanolic extract of H. macrophyllus leaves and their fractions have antibacterial activity against S. aureus. The method used in this antibacterial activity test is the disk diffusion method with a test concentration of 5%, 10%, 15%, 20%, and 25%. The results of this study indicate that all test samples have antibacterial activity except in the water fraction. The highest antibacterial activity was obtained in the ethyl acetate fraction which attracted semipolar compounds in the ethanolic extract of H. macrophyllus leaves. The antibacterial activity of the test samples in sequence from high to low includes ethyl acetate fraction, ethanolic extract, n-hexane fraction, and water fraction.

References

[1] Departemen kesehatan. 2008. Riset Kesehatan Dasar (RISKESDAS) 2007. Jakarta: Departemen kesehatan RI.
[2] WHO. 2015. WHO satistical profil indone-sia. Indonesia: WHO Statistical Profil. 1–3.
[3] WHO. 2007. Epidemic prone and pan-demic prone acute respiratory diseases: infection prevention and control in helath-care facilities. Who. Indonesia Partner in Development. 53(2):8–25.
[4] Kemenkes RI. 2018. Hasil Utama Risk-esdas 2018. Jakarta: Kemenkes RI
[5] Kuswandi M. 2011. Strategi Mengatasi Bakteri yang Resisten terhadap Antibioti-ka. Pengukuhan Jabatan Guru Besar (10-12). Yogyakarta: Fakultas Farmasi Univer-sitas Gadjah Mada.
[6] Tavish MH, DH. dan Martosupono M. 2002. An economic study of essential oil production in the uk: a case study com-paring non-uk lavender/lavandin produc-tion and peppermint/spearmint production with uk production techniques and cost. Adas Consulting Ltd. 137
[7] Agustina RP. 2017. Skrining Fitokimia Dan Uji Aktivitas Antibakteri Ekstrak Eta-nol Daun Waru Gunung (H. macrophyllus Roxb. Ex Hornem) Terhadap Bacillus ce-reus. Skripsi. Jember: Fakultas Farmasi Universitas Jember.
[8] Sudjana. 2000. Metode Statistika. Tarsito, Bandung
[9] CLSI. 2012. Methods for Dilution Antimi-crobial Susceptibility Test for Bacteria That Grow Aerobically; Approved Stand-ard-Ninth Edition. Wayne. 2. Clinical and Laboratory Standards Institute.Robinson, T., 1995, Kandungan Organik Tumbuhan Tinggi, Edisi VI, Hal 191-216, Diter-jemahkan oleh Kosasih Padmawinata, ITB, Bandung.
[10] Sarker SD, Latif Z, dan Gray AI. 2006. Natural Product Isolation Second Edition. New Jersey, USA: Human Press.
[11] Robinson T, 1995, Kandungan Organik Tumbuhan Tinggi, Edisi VI, Hal 191-216, Diterjemahkan oleh Kosasih Padmawina-ta, ITB, Bandung.
Published
2021-01-16
How to Cite
MAULANA, Afif Rifqie; TRIATMOKO, Bawon; HIDAYAT, Mochammad Amrun. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Waru Gunung (Hibiscus macrophyllus) dan Fraksinya terhadap Staphylococcus aureus. Pustaka Kesehatan, [S.l.], v. 9, n. 1, p. 48-53, jan. 2021. ISSN 2721-3218. Available at: <https://jurnal.unej.ac.id/index.php/JPK/article/view/16432>. Date accessed: 01 aug. 2021. doi: https://doi.org/10.19184/pk.v9i1.16432.