Pengaruh Cekaman Salinitas Terhadap Aktivitas Katalase dan Pertumbuhan Bibit Melinjo (Gnetum gnemon L.)
Abstract
ABSTRACT
Melinjo plant (Gnetum gnemon L.) is one of the plants that has the potential to be developed, especially in
the tropics. Melinjo plants have a protein content of 9-10%. Reactive Oxygen Species (ROS) have increased
accumulation in plant cells offset by increased antioxidants, which serve to protect against cell damage caused
by osmotic pressure in plant tissue. Salinity stress can be induced through the application of Sodium Chloride
(NaCl). This research was conducted to determine the response of growth and activity of the enzyme catalase
(CAT) of melinjo seeds to the duration of NaCl application. The study was conducted from January to
completion in the Green House and plant analysis laboratory at the Faculty of Agriculture, University of Jember.
The experimental method used was the Completely Randomized Design (CRD) of NaCl application treatment of
0, 5, 10, 15, and 20%. with a treatment duration of application of 45 days and repeated as many as 5 replications.
NaCl application of 0 - 20% significantly affects plant growth, especially on plant height, root length, and wet
weight parameters. When the condition of the plant experiences salinity stress, it affects the percentage of acid
reduction 2,2'- Azinobis (3-ethylbenzatiazolin) - 6-sulfonate (ABTS) has the highest value in the P3 treatment of
26.08%. The highest CAT antioxidant activity in P4 treatment was 1.02 units / mg protein.
Keywords: NaCl, protein, antioxsidant, Gnetum gnemon L.
ABSTRAK
Tanaman melinjo (Gnetum gnemon L.) merupakan salah satu tanaman yang berpotensi dikembangkan
khususnya di daerah tropis. Tanaman melinjo mempunyai kandungan protein sebesar 9-10%. Reactive Oxygen
Spesies (ROS) mengalami peningkatan akumulasi didalam sel tanaman diimbangi dengan meningkatnya
antioksidan, yang berfungsi melindungi dari kerusakan sel akibat dari tekanan osmotik didalam jaringan
tanaman. Cekaman salinitas dapat diinduksikan melalui aplikasi Natrium Chlorida (NaCl). Penelitian ini
dilakukan untuk mengetahui respon pertumbuhan dan aktivitas enzim katalase (CAT) bibit melinjo terhadap
lama aplikasi NaCl. Penelitian dilaksanakan pada bulan Januari sampai selesai di Green House dan laboratorium
analisis tanaman Fakultas Pertanian Universitas Jember. Metode percobaan yang digunakan adalah RAL
perlakuan aplikasi NaCl 0%, 5%, 10%, 15%, dan 20% dengan perlakuan lama aplikasi 45 hari dan diulang
sebanyak 5 ulangan. Lama aplikasi NaCl 0% - 20% berpengaruh nyata terhadap pertumbuhan tanaman
khususnya pada parameter tinggi tanaman, panjang akar, berat basah tanaman. Pada saat kondisi tanaman
mengalami stress salinitas mempengaruhi persentase peredaman Asam 2,2’- Azinobis (3-etilbenzatiazolin)- 6-
sulfonat (ABTS) memiliki nilai tertinggi pada perlakuan P3 sebesar 26,08%. Aktivitas antioksidan CAT tertinggi
pada perlakuan P4 sebesar 1,02 unit/mg protein.
Kata Kunci: NaCl, protein, antioksidan, melinjo
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