Pengendalian Penyakit Layu pada Pisang dengan Bakteri Antagonis Pseudomonas fluorescens dan Bacillus subtilis

Abstract

Fusarium wilt on bananas caused by the fungus Fusarium oxysporum f.sp cubense were
important and were distributed most of the centre of bananas farm in Indonesia. Both Culture
technicaly and chemically were unable to control this pathogen because they ability to form
Clamydospore and to infect alternative hosts. The use of antagonistic bacteria such as Pseudomonas
fluorescens and Bacillus subtilis were able to control plant pathogens both in rhizosphere or in
phyllosphere because their ability to produce secondary metabolites such as siderophore, antibiotics,
volatile substances, Cyanide acid, extracellular enzymes and phytohormones. This research was
conducted in Laboratory of Plant Pathology and greenhouse of Pest and Plant Diseases Department.
The steps of research included exploration of antagonistic bacteria using Schaad methods,
identification using Schaad, Palleron, Lelliot and Stead Methods. Last steps were done by in vitro and
in vivo tests.The result showed that combination of two bacterias were able to inhibit the pathogen in
vitro (70,2% - 88,1%), while in vivo test with three to four applications in frequency were able to
decrease disease intensity up to 81, 6%.