MOLECULAR CHARACTERIZATION OF Piper retrofractum Vahl IN JAVA USING INTER SIMPLE SEQUENCE REPEATS (ISSR) MARKERS

  • Sulifah Aprilya Hariyani Universitas Jember
  • Siti Zubaidah Universitas Negeri Malang
DOI: https://doi.org/10.19184/bioedu.v20i1.27691

Abstract

Piper is a genus of the family Piperaceae which has a very large number, in the tropics and sub-tropics. There are about 22 species of Piper which are registered in medicinal herbs and world herbs, one of which is Java chili (Piper retrofractum Vahl.). The morphological characters of P. retrofractum are different in various planting centers, especially the leaf and fruit characters vary. Limitations of morphological characters encourage the use of molecular characters. The purpose of this study was to analyze the molecular character of Java chilli (P. retrofractum) on the island of Java using ISSR (Inter simple sequence repeat) markers. Molecular markers can provide relatively more accurate information because genetic traits tend to be stable in environmental changes and not influenced by age. Leaves from 12 different locations were isolated using the SDS method. Amplification was carried out using 12 ISSR polymers. Data is analyzed using the NTSYS version 2.02 program. The results showed that the amplification of P. retrofractum DNA produced 70 polymorphic DNA bands. The dendrogram results from 12 sampling locations showed that P. retrofractum on Java Island had a similarity coefficient of 0.69-0.93. This shows that genetic diversity based on ISSR markers from P. retrofractum Vahl is relatively low.

References

Archibald, F.S., Bourbonnais, R., Jurasek, L., Paice, M.G., and Reid, I.D., 1997. Kraft pulp bleaching and delignification by Trametes versicolor. Journal of Biotechology 53: 215-336.

Aspan, R., 2008. Taksonomi Koleksi Tanaman Obat Kebun Tanaman Obat Citeureup. BPPOM RI. Jakarta.

Besse, P. (ed), 2014. Molecular Plant Taxonomy Methods and Protocols. Spinger. London.

Bolaric S, Barth S, Melchinger A, Posselt U. 2005. Genetic Diversity among Polish landraces of common out (Avena sativa L.) Genet Resour Crop Evol. 60:2157-2169.

Djè Y., Tahi C.G., Zoro Bi AI, Baudoin J.P., Bertin. P., 2010. Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces. Sci Hortic. 124:159–164. doi: 10.1016/j.scienta.

Dyer, L. A., and Palmer, A. D. N., 2004. Piper: A Model Genus for Studies of Phytochemistry, Ecology, and Evolution. Kluwer Academic / Plenum Publishers. New York

Haryudin, W., dan Rostiana, O., 2009. Karakteristik Morfologi Cabe Jawa (Piper retrofractum. Vahl. Di Beberapa Sentra Produksi. Buletin. Littro. Vol 20 No.1, hal.1-10.

Hesse, M., Halbritter, H., Zetter, R., Weber, M., Buchner R., Radivo A. F., Ulrich S. 2009. Pollen Terminology An illustrated handbook. Springer. Austria
Jaramillo, M.A., Callejas, R., Davidson, C., Smith, J.F., Stevens, A.C., Tepe, E. J., 2008. Systematic Botany 33 (4): pp. 647-660.

Jiang, Y. dan Liu, Jin-Ping. 2011. Analysis of Genetic Diversity of Piper spp. In Hainan Island (China) using Inter-Simple Sequence Repeat (ISSR) markers. African Journal of Biotechnology Vol. 10 (66) pp.14731-14737.
Julianisah, I.N., Sulistyowati, L., Sugiharto, N.A., 2008. Analisis kekerabatan mentimun (Cucumis sativus L) menggunakan metode RAPD-PCR dan Isozim.Biodiversitas. 9:99–102.

Khare, C.P., 2007. Indian medicinal plants. 1st edition. Springer Science Business Media, LLC., 16–17.

Kumar P, Gupta VK, Misra AK, Modi DR, Pandey BK. 2009. Potential of molecular markers in plant biotechnology. Plant Omi J. 2:141-162.

Mondini L., Noorani A., Pagnotta M.A., 2009. Assesing Plant Genetic Diversity by Molecular Tools. Diversity 1: 19-35.

Moulin MM, Rodrigues R, Simoes L, Goncalves A, Pereira G. 2012. A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.). Acta Sci. 34:139– 147.

Ninot, A. and Aletta, N., 2003. Identification and genetic relationship of persian walnut genotypes using isozyme markers. Journal of the American Pomological Society 57:106-114.

Pandit SS, Mitra S, Giri AP, Pujari KH, Patil BP, Jambhale ND, Gupta VS. 2007. Genetic diversity analysis of mango cultivars using inter simple sequence repeat markers. Current Sci. 93:1135–1141.

Parmar. V. S., Jain, S.C., Bisht, K S., Jain R., Taneja, P., Jha A, Tyagi. O. D., Prasad, A. K., Wengel, J, E T Carl. Olsen And Per. 1997. Phytochemistry of The Genus Piper. Phytochemisrry, Vol. 46, No. 4, pp. 591-673, 1997. Elsevier Science. Pergamon.

Prasad, M.P., Shekhar, S., Amit, B., 2012. Phytochemical Analysis and Antioxidant potential of Piper species and its Molekuler Characterization by RAPD Markers. International Jounal of Fundamental & Applied Sciences. Vol 1. No.4 p. 71-73.

Radford, A.E., 1986. Fundamental of Plant Systematics. London: Longman Higer Education.

Rohlf FJ. 1998. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. New York (US): Exeter software.

Ziekiewicz, E., A. Rafalski, D. Labuda. 1994.Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification. Genome, 20: 176-183.

Zulfahmi. 2013. Penanda DNA untuk analisis genetic tanaman. Jrnal Agroteknologi 3(2): 41-52.
Published
2022-06-27
How to Cite
HARIYANI, Sulifah Aprilya; ZUBAIDAH, Siti. MOLECULAR CHARACTERIZATION OF Piper retrofractum Vahl IN JAVA USING INTER SIMPLE SEQUENCE REPEATS (ISSR) MARKERS. BIOEDUKASI, [S.l.], v. 20, n. 1, p. 1-7, june 2022. ISSN 2580-0094. Available at: <https://jurnal.unej.ac.id/index.php/BIOED/article/view/27691>. Date accessed: 22 dec. 2024. doi: https://doi.org/10.19184/bioedu.v20i1.27691.
Citation Formats
Issue
Vol 20 No 1 (2022)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.