Bioinformatics Analysis to Construct Cellulose-binding Module Synthetic Gene and Design Primer

  • Febriana Dwi Wahyuni University of Esa Unggul
  • Asrul Muhamad Fuad Research Center for Biotechnology, LIPI, Indonesia

Abstract

Cellulose-binding module (CBM) is a protein domain commonly found in various types of cellulase enzymes. The function of this CBM can be used for the binding process and the immobilization of a protein in the cellulose matrix. CBM can be obtained from several organisms, one of them is Trichoderma reesei. To get a gene, it does not have to be isolated from the original organism. Gene sequences can be obtained synthetically through bioinformatics analysis in accordance with the same gene sequences as those at Gene Bank. Bioinformatics analysis can be used to find new gene sequences or existing genes. This study aims to get a cbmsyn synthetic gene quickly and efficiently without reducing protein activity, which can then be ligated with other genes so that it functions as an immobilized enzyme. From the results of bioinformatics analysis, obtained DNA sequences measuring around 498 pb with 166 amino acid protein lengths. The sequence was modified by adding several restriction sites, namely BamHI, AfeI, and ScaI. The DNA sequences obtained were optimized with the Pichia pastoris codon.

Published
2019-04-01
How to Cite
WAHYUNI, Febriana Dwi; FUAD, Asrul Muhamad. Bioinformatics Analysis to Construct Cellulose-binding Module Synthetic Gene and Design Primer. BIOEDUKASI, [S.l.], v. 17, n. 1, p. 40-44, apr. 2019. ISSN 2580-0094. Available at: <https://jurnal.unej.ac.id/index.php/BIOED/article/view/13204>. Date accessed: 30 mar. 2024. doi: https://doi.org/10.19184/bioedu.v17i1.13204.
Section
Articles

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.