ELEPHANTOPUS SCABER AND SAUROPUS ANDROGYNUS REGULATE MACROPHAGES AND B LYMPHOCYTE CELLS DURING SALMONELLA TYPHI INFECTION

Muhammad Sasmito Djati; Dinia Rizqi Dwijayanti; Lulut Dwi Nurmamulyosari; Yayu Fuadah; Muhammad Basyarudin; Nur Jannah

Muhammad Sasmito Djati Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang
Dinia Rizqi Dwijayanti Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang
Lulut Dwi Nurmamulyosari Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang
Yayu Fuadah Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang
Muhammad Basyarudin Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang
Nur Jannah Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang

Abstract

Abstract—Macrophages and B lymphocyte play an important role as the first cell type to encounter bacterial pathogen and as a mediator that initiate the adaptive immune response. Those types of cell can die in many ways such as apoptosis, necrosis, pyroptosis and autophagy during the host cell-pathogen recognition. This study aimed to investigate the ability of E. scaber and S. androgynus formula in regulating macrophage and B lymphocyte cells during bacterial infection. The pregnant mice were randomly divided into seven experimental groups: T1 (control), T2 (S. typhi infection), T3 (S. typhi, E. scaber 100%), T4 (S. typhi, E. scaber 75% and S. androgynus 25%), T5 (S. typhi, E. scaber 50% and S. androgynus 50%), T6 (S. typhi, E. scaber 25% and S. androgynus 75%), and T7 (S. typhi, S. androgynus 100%). Flowcytometry analysis was performed on day 18. S. typhi infection decrease the formation of macrophage and B lymphocyte cells in bone marrow and induce cell death in PBMC. We clearly proved that E. scaber 75% and S. androgynus 25% formula was able to ameliorate the formation of macrophage and B lymphocyte cells in BM. While E. scaber 25% and S. androgynus 75% formula increased the relative number of macrophage and B lymphocyte cells in PBMC.