DETERMINATION OF TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY IN METHANOLIC EXTRACT OF ROBUSTA AND ARABICA COFFEE LEAVES

Abstract

Phenols contained in coffee beans may also found in
the coffee leaves. Previous research showed that the
arabica coffee leaves contain phenolic compounds
[1]. Phenolic compounds are compounds that have
one or more hydroxyl groups attached directly to an
aromatic ring [2]. Phenolics compounds have many
biological effects, including antioxidant activity [3].
Antioxidants are the first line for defense against
free radical damage, and important for health. The
need for antioxidants becomes even more critical
with increased exposure to free radicals. Pollution,
cigarette smoke, drugs, illness, stress, and even
exercise can increase free radical exposure [4].
The aim of this study was to determine total
phenolic content and antioxidant activity in
methanolic extracts of robusta and arabica coffee
leaves (old and young leaves), as potential sources of
natural antioxidants. The relationship between
phenolic content and antioxidant activity was also
investigated.