INHIBITORY EFFECT OF NON-POLAR AND SEMI-POLAR FRACTIONS OF ETHANOLIC EXTRACT OF Guazuma ulmifolia Lamk. LEAVES ON RAT PREADIPOCYTES PROLIFERATION AND DIFFERENTIATION

Abstract

Jati Belanda Leaves (G. ulmifolia Lamk.) has been
used traditionally to reduce obesity 1). G. ulmifolia
Lamk. leaves extract contained alkaloids, tannins,
saponins, flavonoids, terpenoids, glycosides, and
steroids 2). Phytochemical screening results
conducted by Iswantini et al. showed that the
flavonoids and tannins were detected high levels in
this extract 3). Flavonoids contained in the leaves of
G. ulmifolia Lamk. were catechin, kaempferol
glycosides, procyanidin B-2, procyanidin B-5,
procyanidin C-14).
Obesity can occur when the body of excess adipose
tissue (fat), particularly white adipose. Excess white
adipose tissue (WAT) is considered as the main
cause of obesity 5). The growth of adipose tissue
occurs due to the formation of new adipocytes and
increase in size of adipocytes, called adipogenesis.
Adipogenesis involves the multiplication of cells
(proliferation) and cell maturation (differentiation).
The treatment which can affect the size and number
of adipocytes and signals expression are involved in
the inhibition or stimulation of specific adipokines
have been used to prove the bioactivity associated
with antiobesity 6).
Natural products that have a specific target in this
pathway have the potential to prevent and treat
obesity. Some polyphenols, including flavonoids
have anti-obesity effects and also specific effects on
adipocytes 7). The presence of the flavonoids i.e.
catechin, kaempferol, tilirosida, and prosianidin in G.
ulmifolia Lamk leaves. may affect adipogenesis.
This research will be conducted extraction and
fractionation leaves of G. ulmifolia Lamk. Extracts,
non-polar fractions and semi-polar fractions tested
their effect on the proliferation and differentiation
of Wistar rat preadiposit.