EFFECTIVENESS OF BINTARO (Cerberra odollam Gaertn.) LEAF ETHANOLIC EXTRACT AGAINST Staphylococcus aureus IN-VITRO BIOFILM FORMATION

Abstract

Biofilm is a structural form of a group of
microorganisms protected by matrik extracellular
called Extracellular Polymeric Substance (EPS),
where EPS is a product produced by these
microorganisms itself and as a shelter from the
bad influence in environment. The structure of
these matrix is strings which cross each other
which can be adhesive for biofilm4).
Biofilm from Staphylococcus aureus seen in
chronicle infection cases as ulcer on diabetics
foot, venous statis ulcer and pressure sores.
Patients with infection wound with chronicle
ulcer in vein feet found positive culture of
Staphylococcus aureus as much as 88-93,3% from
a number of infection found.
Figure 1. Cerberra odollam Gaertn.
Bintaro can be used as an analgesic,
anticonvulsant, kardiotonik and antihypertensive
2).
The aim of this study is to test the effectiveness
of the ethanol extracts of bintaro leaves (Cerberra
odollam Gaertn.) towards the Staphylococcus
aureus formation by in vitro.
MATERIALS
Bintaro leaves (Cerberra odollam Gaertn.),
Staphylococcus aureus culture ATCC 6538, petri
dish, ose wire , micro pipet, autoclave (All
American Model No 25x, USA), incubator
(Memmert and Binder, Germany), Laminar Air
Flow (LAF) (Type V-130, Indonesia), microplate,
microplate reader (Thermofisher Scientific,
America), vacuum rotary evaporator (BÜCHI,
Germany), weight analytic (Sartorius TE 214 S,
Germany), oven (Memmert, Germany).