PENGARUH VARIASI KONSENTRASI EKSTRAK DAUN KETEPENG CINA (Cassis alata L.) TERHADAP MORTALITAS CACING Ascaris suum DEWASA SECARA IN VITRO

Abstract

Abstract. Suffer from worms is a chronic and endemic disease caused by parasite worm in high prevalence, not deadly, but gnaw the health of the people therefore decrease the nutrient. Ketepeng cina leaf contains active substances such as saponin which is believed to have anthelmintic power. The objective of this research is to know the anthelmintic power of ketepeeng cina leaf extract (Cassia alata L.) on Ascaris suum by in vitro. This research is a laboratory experimental research with two-factor completely randomized design. The samples are divided into six experimental groups. These groups are negative control which is incubated in physiological salt solution, positive control with pyrantel pamoate solution 1%, and experimental groups in ketepeng cina extract solution with 20%, 40%, 60%, and 80% concentration. The mortalities of the worms are counted in each 3 hours of observation until the limit of observation time. Data analysis is done by using variant analysis (ANAVA) two ways. If there is any difference, it is continued by Duncan Test with confidence level 95% (p<0.05), shapiro-wilk normality test, and Probit analysis by using Minitab 14. According to the probit analysis, LC₅₀ of ketepeng cina leaf extract is 39.98%. LT₅₀ ketepeng cina leaf extract with 40% concentration is 11.33 hour, LT₅₀ ketepeng cina leaf extract with 60% concentration is 8.71 hour, and LT₅₀ ketepeng cina leaf extract with 80% concentration is 3.15 hour, while LT₅₀ pyrantel pamoate 1% is 2.94 hour on Ascaris suum by in vitro. From the experiment, it can be concluded that ketepeng cina leaf extract (Cassia alata L.) has anthelmintic power on Ascaris suum by in vitro

Key Words: Ascaris suum, Cassia alata L., extract