Uji Aktivitas Antibakteri Ekstrak Etanol Daun Ungu (Graptophyllum pictum (L) Griff) terhadap Pertumbuhan Streptococcus sanguinis

Nafila Syahrani; Atik Kurniawati; Ayu Mahartini Prihanti; Erna Sulistyani; Pujiana Endah Lestari

doi:10.19184/pk.v12i1.42325

Nafila Syahrani Fakultas Kedpkteran Gigi, Universitas Jember
Atik Kurniawati Fakultas Kedokteran Gigi, Universitas Jember
Ayu Mahartini Prihanti Fakultas Kedokteran Gigi, Universitas Jember
Erna Sulistyani Fakultas Kedokteran Gigi, Universitas Jember
Pujiana Endah Lestari Fakultas Kedokteran Gigi, Universitas Jember

DOI: https://doi.org/10.19184/pk.v12i1.42325

Abstract

Streptococcus sanguinis has strong adhesion with salivary pellicles which causes other oral bacteria to attach more and has contribution in causing caries and periodontal disease. S. sanguinis mediated the adhesion of S. mutans to dental pellicle receptors so inhibiting the interaction between S. sanguinis and S. mutans would prevent the risk of dental caries. Purple leaf (Graptophyllum pictum) is one of the medicinal plants or natural ingredients that are useful as antibacterials. The purpose of this study was to examine antibacterial activity of purple leaf extract against S. sanguinis. This experimental laboratory study with a pre-posttest control group design was conducted by dilution method on treatment groups, purple leaf extract with concentrations of 25%, 12.5%, 6.25% and 3.12%. Visual and spectrophotometer examination was observed before and after incubation.The result showed that purple leaf extract with concentrations of 6.25% and 3.12% and control + groups had not turbidity and decreased absorbance value. Furthermore, the One Way Anova parametric statistical test and also Post-Hoc Least Significant Difference showed that there were significant differences between the study groups. The purple leaf extract (Graptophyllum pictum L. Griff) had antibacterial activity against S. sanguinis. The antibacterial test for extracts that are dark in color can be continued using the TVC (Total Viable Count) method to provide a quantitative estimate of the concentration of bacterial cells in the sample in units of CFU/ml.

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