Pengembangan Biosensor Berbasis Plastik Zona Mikro untuk Skrining Aktivitas Antidiabetes pada Ekstrak Tanaman Obat

Indri Firma Wati; Bambang Kuswandi; Dwi Koko Pratoko

doi:10.19184/pk.v9i1.20423

Indri Firma Wati Fakultas Farmasi Universitas Jember
Bambang Kuswandi Fakultas Farmasi Universitas Jember
Dwi Koko Pratoko Fakultas Farmasi Universitas Jember

DOI: https://doi.org/10.19184/pk.v9i1.20423

Abstract

Research on the diabetes mellitus (DM) treatment has recently been carried out, especially on discovering antidiabetic agents derived from plants. This study aims to determine the fabrication of microzone plastic-based biosensors, determine the optimal sensor conditions, analysis characteristics, and compare the biosensor with the UV-Vis spectrophotometric method. The results showed that the response time was 15 minutes, the linear range was 500-40000 µg / mL (R = 0.9989). The limit of detection (LOD) was 1109.6 µg / mL, and the limit of quantification (LOQ) was 3698.8 µg / mL. Biosensor filled up the precision parameters with an RSD value of less than 3.7% and an accuracy with recovery in the range of 95-105%. The biosensor is stable in storage at 25 ° C for 270 minutes and at chiller temperature for three days. The antidiabetic activity of the biosensor was compared with the UV-Vis spectrophotometer using the Independent Sample T-test and showed insignificant differences between the two methods.

References

1. PERKENI. Konsensus pengelolaan dan pencegahan diabetes mellitus tipe 2 di Indonesia 2015. Jakarta: PB. PERKENI; 2015. 11–14 p.
2. Novia Pegin Wardani. Uji Aktivitas Anti Diabetes Ekstrak Kering Biji Mahoni Terstandar (Swietenia mahagoni Jack) Pada Mencit Yang Diinduksi Aloksan. Skripsi. 2010;(050710106).
3. Marrianne, Yuandani, Rosnani. Antidiabetic Activity from Ethanol Extract of Kluwih’s Leaf (Artocarpus camansi). J Nat. 2011;11(2):64–8.
4. Hadiarti D. Inhibisi α-Glucosidase Ekstrak Etanol Buas-Buas (Premna serratifolia) secara In Vitro. Maj Obat Tradis. 2017;22(2):80.
5. Holidah D, Yasmin, Christianty FM. Uji Aktivitas Antidiabetes Ekstrak Teh Hitam dan Teh Hijau secara In Vitro Menggunakan Metode Inhibisi Enzim α-Glukosidase ( In Vitro Antidiabetic Activity of Black Tea and Green Tea Extracts by Inhibition of α-Glucosidase Method ). e-Jurnal Pustaka Kesehat. 2018;6(2):235–9.
6. Mahargyani W. Uji Aktivitas Antidiabetes Ekstrak n-Heksan Kulit Buah Naga Merah (Hylocereus polyrhyzus). EduChemia (Jurnal Kim dan Pendidikan). 2019 Jan 31;4(1):13–23.
7. Ariani N, Kartika IR, Kurniadewi F. Uji Aktivitas Inhibisi Enzim α-Glukosidase secara In Vitro dari Ekstrak Metanol Daun Cryptocarya densiflora Blume dan Fraksi-Fraksinya Nurul. 2017;7(1):1–6.
8. Bhalla N, Jolly P, Formisano N, Estrela P. Introduction to biosensors. Essays Biochem. 2016;60(1):1–8.
9. Kawamura A, Miyata T. Biosensors: An Overview. In: Biomaterials Nanoarchitectonics. Osaka: Kansai University; 2016. p. 157–76.
10. Mohiuddin M, Arbain D, Islam AKMS, Ahmad MS, Ahmad MN. Alpha-Glucosidase Enzyme Biosensor for the Electrochemical Measurement of Antidiabetic Potential of Medicinal Plants. Nanoscale Res Lett. 2016;11(1):1–12.
11. Guo S, Lin X, Wang Y, Gong X. Fabrication of paper-based enzyme immobilized microarray by 3D-printing technique for screening α-glucosidase inhibitors in mulberry leaves and lotus leaves. Chinese Med (United Kingdom). 2019;14(1):1–11.
12. Yuwono M, Indrayanto G. Validation of Chromatographic Methods of Analysis. Profiles Drug Subst Excipients Relat Methodol. 2005;32(05):241–60.
13. Caesar LK, Cech NB. Synergy and antagonism in natural product extracts: When 1 + 1 does not equal 2. Nat Prod Rep. 2019;36(6):869–88.
14. Berawi KN, Shidarti L, Nurdin SU, Lipoeto NI, Wahid I, Sari J, et al. Comparison effectiveness of antidiabetic activity extract herbal mixture of soursop leaves(annona muricata), bay leaves(syzygium polyanthum) and pegagan leaves(centella asiatica). Biomed Pharmacol J. 2017;10(3):1481–8.
15. Widharna R, Ferawati, Tamayanti W, Hendriati L, Hamid I, Widjajakusuma E. Antidiabetic Effect of the Aqueous Extract Mixture of Andrographis paniculata and Syzygium polyanthum Leaf. European J Med Plants. 2015;6(2):82–91.
16. Lu G, Tan HY, Neild A, Liew OW, Yu Y, Ng TW. Liquid filling in standard circular well microplates. J Appl Phys. 2010;108(12).