Increased Interleukin-4 in Farmers With Ascariasis in Rural Jember

  • Adiz Dwiputra Rahmadhan Fakultas Kedokteran, Universitas Jember
  • Yunita Armiyanti Fakultas Kedokteran, Universitas Jember
  • Bagus Hermansyah Fakultas Kedokteran, Universitas Jember
  • Dina Helianti Fakultas Kedokteran, Universitas Jember
  • Yudha Nurdian Fakultas Kedokteran, Universitas Jember

Abstract

Ascaris lumbricoides is nematode which often infest human in the world. The infestation of A. lumbricoides in the human will trigger the formation of Th2 immune responses through increased interleukin-4 released by innate immune cells. Th2 immune response generates B lymphocyte cells to produce IgE antibodies to eliminate A. lumbricoides. Therefore, IL-4 is a marker of Th2 immune response. The purpose of this study was to determine the prevalence of ascariasis and an increase of IL-4 in planters infested with A. lumbricoides as a marker of Th2 immune response. Our study was observational analytic with cross sectional design. 230 planters who were willing to be involved in the study gave stool samples for the diagnosis of ascariasis. 20 people who were positive for ascariasis and 20 people who were negative selected randomly for blood plasma samples. IL-4 levels in blood plasma were measured using ELISA. Differences in IL4 levels between positive and negative ascariasis were analyzed by the Mann Whitney statistical test with p value ≤ 0.05. The prevalence of ascariasis among planters in rural area of Jember was 16,52% which was included low prevalence. Univariate analysis showed that the mean and median levels of IL-4 ascariasis were greater than those of healthy planters. Mann Whitney test results showed that there were differences in levels of IL-4 between ascariasis and healthy planters (p≤0.05). Increased levels of IL-4 in planters with ascariasis indicate that A. lumbricoides infection triggers the formation of Th2 immune response as a mechanism for eliminating A. lumbricoides.
Keywords: Interleukin-4, A. lumbricoides, Th2 immune response.

References

Betson M & Stothard, JR. 2016. Ascaris lumbricoides or Ascaris suum: What′s in a Name?, J Infect Dis, 213(8):1355–1356. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799673

Bundy DAP, Silva N, Horton S, Hamison DJ,. Patton G C & Brown G. 2017. Child and Adolescent Health and Development. 3rded. Washington, DC: World Bank Development), p. 265.

Cooper P, Barreto M & Rodrigues L. 2006. Human Allergy and Geohelminth Infections: A Review of the Literature and a Proposed Conceptual Model to Guide the Investigation of Possible Causal Associations. British Medical Bulletin: 79–80;203–218. Available at:https://pubmed.ncbi.nlm.nih.gov/17204485/#affiliation-1.

Eidwina CA, Faridah L, Ermaya YS & Gurnida DA. 2016. Association of Ascariasis with Nutritional and Anemic Status in Early School-age Students. Althea Medical Journal. 3(1):93–98.

Gassani A. 2011. Hubungan Cacing Usus STH dengan Kebiasaan Bermain Tanah pada SDN 09 Pagi Paseban Tahun 2010. Jakarta: Fakultas Kedokteran Universitas Indonesia.

Gazzinelli-Guimaraes PH & Nutman TB. 2018. Helminth Parasites and Immune Regulatio.F1000Res. 2018;7:F1000 Faculty Rev-1685.doi: 10.12688/f1000research.15596.1.

Goddey NOP, Osagie ID & Maliki A. 2010. Serum cytokines profiles in Nigerian children with Ascaris lumbricoides
infection. Asian Pacific Journal of Tropical Medicine. Hainan Medical College. 3(4): 288–291. doi: 10.1016/S1995-7645(10)60070-3.

Kemenkes, R. 2017. Peraturan Menteri Kesehatan Republik Indonesia Tentang Penanggulangan Cacingan. Jakarta.

Luzina IG, Keegan AD, Heller NM, Rook GAW, Shea-Donohue T & Atamas SP. 2012. Regulation of inflammation by interleukin-4: a Review of “alternatives”. Journal of Leukocyte Biology. 92(4):753–764.

Masure D, Vlaminck J, Wang T, Chiers K, Van den Broeck W, Vercruysse J & Geldhof P. 2013. A role for Eosinophils in The Intestinal Immunity AgainstInfective Ascaris suum larvae. PLoS Neglected Tropical Diseases. 7(3):e2138.

Mendoza A. 2017. Genetic Regulation of the Antibody Response to the Parasite Ascarisspp. Colombia: University of Cartagena.

Motran CC, Silvane L, Chiapello LS, Theumer MG, Ambrosio LF, Volpini X, Celias DP & Cervi L.. 2018. Helminth Infections: Recognition and Modulation of The Immune Response by Innate Immune Cells. Frontiers in Immunology.9:664.

Nurdian Y & Kurniawati H. 2005. Identifikasi Kontamiasi Telur dan Larva Cacing Parasit pada Tanah di Daerah Perkebunan Mumbulsari, Kabupaten Jember. Jurnal Biomedis. 3:5–29.

Primadana A, Nurdian Y, Agustina D, Hermansyah B, & Armiyanti Y. 2019. Eosinophilia sebagai Prediktor Morbiditas Soil-Transmitted Helminthiases pada Pekerja Perkebunan Widodaren, Jember.Journal of Vocational Health Studies. 03: 47-52.

Pullan RL, Smith JL, Jasrasaria R & Brooker SJ. 2014. Global Numbers of Infection and Disease Burden of Soil Transmitted Helminth Infections in 2010. Parasites & Vectors. 7(1):37

Sampe S. 2019. Gambaran jumlah eosinofil pada pekerja perkebunan gunung pasang dan kaliputih yang terinfestasi soil-transmitted helminthes. Universitas Jember.

Shalaby, Naglaa MS, & Nehad M. 2016. Effect of Ascaris lumbricoides Infection on T Helper Cell Type 2 in Rural Egyptian children. Therapeutics and Clinical Risk Management. 12:379–385. doi: 10.2147/TCRM.S94019.

Ul-Haq Z, Naz S & Mesaik M. 2016. Interleukin-4 Receptor Signaling and Its Binding Mechanism: A Therapeutic Insight from Inhibitors Tool Box.Cytokine Growth Factor Rev. 32:3–15. doi: doi:10.1016/j.cytogfr.2016.04.002.

Wang H, Naghavi M, Allen C, Carter A, Casey DC, Charlson FJ, Chen AZ & Coates MM. 2016. Global, Regional, and National Life Expectancy, All-cause Mortality, and Cause-specific Mortality for 249 Causes of Death, 1980–2015: a Systematic Analysis for the Global Burden of Disease Study 2015. The Lancet. 388(10053):1459–1544.

Weatherhead J, Cortes AA, Sandoval C, Vaca M, Chico M, Loor S, Cooper PJ & Mejia R. 2017. Comparison of Cytokine Responses in Ecuadorian Children Infected with Giardia, Ascaris, or Both Parasites.Am J Trop Med Hyg. 96(6):1394–1399. doi: doi:10.4269/ajtmh.16-0580.

Wijaya NH, Anies, Hadisaputro S.& Setyawan HS.2016.Faktor Risiko Kejadian Infeksi Cacing Tambang pada Petani Pembibitan Albasia di Kecamatan Kemiri Kabupaten Purworejo. JEEK. Jurnal Epidemiologi Kesehatan Komunitas. 1(1):15-24.
Published
2021-01-11
How to Cite
RAHMADHAN, Adiz Dwiputra et al. Increased Interleukin-4 in Farmers With Ascariasis in Rural Jember. Jurnal ILMU DASAR, [S.l.], v. 22, n. 1, p. 25-30, jan. 2021. ISSN 2442-5613. Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/15921>. Date accessed: 24 nov. 2024. doi: https://doi.org/10.19184/jid.v22i1.15921.
Section
General