Effect of Black Soybean (Glycine soja) Extract on Ovarian Histology of Swiss Webster Mice (Mus musculus L) Unilateral Ovariectomy

  • Mahriani Mahriani Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember
  • Eva Tyas Utami Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember
  • Nindita Fitria Primasari Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Abstract

Estrogen production can be reduced due to menopause and ovariectomy. Decreased estrogen levels in the body cause physiological changes in the female reproductive system. Therefore, the intake of estrogen from outside the body is needed, in this case phytoestrogens from soy bean extract can be used to replace the role of endogenous estrogen. The study used 30 Swiss Webster mice (Mus musculus) aged 90 days weighing 35 grams which were unilateral ovariectomy. Mice are ready to be used as test animals after an estrogen deficiency period of 60 days. Mice are ready to be used as test animals after an estrogen deficiency period of 60 days. Mice were divided into five groups: negative control, positive control, dose 0.21, 0.42, and 0.63 g/mL/day . Data obtained from the results of the study were analyzed using the One Way ANOVA test with a confidence level of 99% or α = 0.01. Based on the results of the study, the administration of black soybean flour extract in unilateral ovariectomy mice can increase the average number of primordial follicles at doses of 0.42 g/ml/day and dosage 0.63 g/ml/day, primary follicles at a dose of 0.42 g/ml/day and doses of 0.63 g/ml/day, and secondary follicles at a dose of 0.63 g / ml / day, and tend to increase the average number of primordial follicles at doses of 0.21 g/ml/day, follicles primary at doses of 0.21 g/ml/day, secondary follicles at doses of 0.21 g/ml/day and 0.42 g/ml/day, de Graaf follicles at doses of 0.21, 0. 42 and 0.63 g/ml/day.

References

Berkalani YK. 2013. Pengaruh Pemberian Infusa Biji Adas (Foeniculum vulgare Mill.) Terhadap Perkembangan Folikel Ovarium Tikus Putih (Rattus norvegicus) Produktif Dan Premenopouse. Skripsi. Fakultas Kedokteran Hewan Institut Pertanian Bogor. Bogor.

Cassidy A, Bingham S & Setchell K. 1995. Biological Effects of Isoflavones In Young Women: Importance of The Chemical Composition of Soyabean Products. British Journal of Nutrition. 74: 587-601.

Couse JK & Korach KS.1999. Estrogen Receptor Null Mice: What Have We Learned and Where Will They Lead Us? Endocrine Review. 20(3): 358-417.

Dewantiningrum, J. 2008. Perbedaan Pengaruh Clomiphene Citrate dan Letozole Terhadap Folikel, Endometrium dan Lendir Serviks. Tesis. Semarang: Program Pendidikan Dokter Spesialis I Universitas Diponegoro.

Findlay JK. Kerr JB, Britt K, Liew SH, Simpson ER, Rosairo D & Drummond A. 2009. Ovarian Physiology: Follicle Development, Oocyte and Hormone Relationship. Anim. Reprod. 6(1): 10-19.

Hafez, B. & Hafez, E. 2000. Reproduction in Farm Animal 7th Edition. USA. Lippincott William & Wilkins : Baltimore.

Hwang KA & Choi. 2015. Endocrine-Disrupting Chemicals with Estrogenicity Posing the Risk of Cancer Progression in Estrogen-Responsives Organ. Advances in Molecular Toxycology. 9.

Kolibianakis EM, Papanikolau EG, Fatemi HM & Devroy P. 2005. Estrogen and Folliculogenesis:Is One Necessary for The Other? Curr.Opin.Obstet.Gynecol. 17: 249-253.

Lestari DW. 2018. Efek Pemberian Ekstrak Tepung Kedelai Hitam (Glycine soja) terhadap Implantasi Mencit (Mus musculus) Strain Balb/C Pasca Ovariektomi Unilateral. Skripsi. Program Studi S1-Biologi. Jurusan Biologi FMIPA Universitas Jember.

Mardianti SM & Sitasiwi AJ. 2008. Korelasi Jumlah Folikel Ovarium dengan Konsentrasi Hormon Estrogen Mencit (Mus musculus) setelah Konsumsi Harian Tepung Kedelai selama 40 Hari. Buletin Anatomi dan Fisiologi. XVI(2).

Markaverich BM, Webb B, Densmore CL & Gregory RR. 1995. Effect of Coumestrol on Estrogen Reseptor Function and Uterin Growth in Ovarietomized Rats. Environ Health Perspect, 103(6): 574-581.

Mazur WM, Duke JA, Wahala K, Rasku S & Adlercretutz H. 1998. Isoflavonoids and Lignans in Legumes: Nutritiol and Health Aspect in Humans. Research Communications. 9: 193-200.

Meredith S, Dudenhorffer G, Butcher RL, Lener S P & Walls T. 1992. Unilateral Ovariectomy Increases Loss of Primordial Follicles and is Associated with Increased Metestrus Concentration of Follicles Stimulating Hormone in Old Rats. Biology of Reproduction. 47: 162-168.

Moon YJ, X Wang & Morris ME. 2006. Dietary flavonoids: Effects of Xenobiotic and Carcinogen Metabolism. Toxicol. In Vitro. 20: 187-210.

Ren S-C, Liu ZL & Wan, P. 2011. Proximate composition and flavonoids content and in vitro antioxidant activity of 10 varieties of legume seeds grown in China. Journal of Medicinal Plants Research. 6(2): 301-308.

Rul AS, Soejono SK, Meiyanto E & Wahyuningsih MSH. 2016. Efek Kurkumin Terhadap Estradiol dan Ekspresi Reseptor Estrogen β Kultur Sel Granulosa Babi Folikel Sedang. Jurnal Kedokteran Brawijaya. 29(1): 32-38.

Sirtori CR, Arnoldi A & Johnson. 2005. Phytoestrogens: End of a Tale?. Ann.Med. 37: 423-438.

Strom JO, Theodorsson A, Ingberg E, Isaksson IM & Theodorsson E.2012. Ovariectomy and 17 β-estradiol Replacement in Rats and Mice: a Visual Demonstration. Journal of Visualized Experiments. 64: 1-4.

Whitten PL & Pattisaul HB. 2001. Cross-species dan interassay Comparison of Phytoestrogen Action. Environmental Health Perspectives Supplements.109. Departmen Anthropology and Center for Behavioural Neuroscience Emory University. Atlanta. Georgia USA.

Xu BJ & Chang SKS. 2007. A Comparative Study on Phenolic Profils and Antioxidant of Legums as Affected by Extraction Solvents. J. Food Sci. 72(2): 159-166.
Published
2022-01-14
How to Cite
MAHRIANI, Mahriani; UTAMI, Eva Tyas; PRIMASARI, Nindita Fitria. Effect of Black Soybean (Glycine soja) Extract on Ovarian Histology of Swiss Webster Mice (Mus musculus L) Unilateral Ovariectomy. Jurnal ILMU DASAR, [S.l.], v. 23, n. 1, p. 37-42, jan. 2022. ISSN 2442-5613. Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/10787>. Date accessed: 05 july 2022. doi: https://doi.org/10.19184/jid.v23i1.10787.
Section
General