The Expression of The PfEMP1-DBL2β Recombinant Protein of Plasmodium falciparum Isolated From Indonesia

Fathul Hidayatul Hasanah; Erma Sulistyaningsih; Widhi Dyah Sawitri

doi:10.19184/jid.v21i1.10494

Fathul Hidayatul Hasanah Post-Graduate of Biotechnology University of Jember
Erma Sulistyaningsih Post-Graduate of Biotechnology, University of Jember, Faculty of Medicine University of Jember, Center for Development of Advanced Sciences and Technology (CDAST) University of Jember
Widhi Dyah Sawitri Post-Graduate of Biotechnology University of Jember, Center for Development of Advanced Sciences and Technology (CDAST) University of Jember

DOI: https://doi.org/10.19184/jid.v21i1.10494

Abstract

The binding of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) to Intracellular Adhesion Molecule-1 (ICAM-1) is a major pathological mechanism in severe malaria including cerebral malaria. The binding is mediated by PfEMP1-DBL2β domain. The study aimed to explore there combinant protein of PfEMP1-DBL2β domain of P. falciparum isolated from Indonesia. DNA was isolated from a severe malaria patient. The DBL2β domain was amplified using Polymerase Chain Reaction (PCR) with specific primer and cloned into the pJET1 cloning vector. The DBL2β recombinant protein was constructed from DBL2β-pJET1 clone using pET-30a expression vector and expressed in Escherichia coli BL21-DE3. PCR colony and digestion of plasmid clones using restriction enzymes were conducted to confirm cloning result, and the expression of recombinant protein was analyzed using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The expression of DBL2β-PfEMP1 domain is higher in pellet than in supernatant fraction. In conclusion, the DBL2β-PfEMP1 domain recombinant protein of P. falciparum isolated from Indonesia expressed as a ~66 kDa protein in full length.
Keywords: DBL2β domain, Indonesia, PfEMP1, Plasmodium falciparum, recombinant protein.

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