• OPTIMIZATION OF SUBCRITICAL WATER ASSISTED BY NITROGEN PRIOR TO ENZYMATIC HYDROLYSIS FOR REDUCING SUGAR PRODUCTION
    Vol 1 No 1 (2020)

    Maktum Muharja, Nur Fadhilah, Rizki Fitria Darmayanti, Dwina Moentamaria and Arief Widjaja

     

    Abstract

    The objective of this study is to optimize the three significant parameters of the subcritical water (SCW) process for sugar production from coconut husk by using response surface methodology (RSM). In this study, the effect of temperature, reaction time and solvent-feed (S/F) ratio was evaluated and discussed comprehensively. The results showed that the S/F ratio had the most significant effect on sugar yield. The optimal sugar yield was obtained at the highest S/F ratio of 23.4, the shortest time of 4.8 min, and the highest temperature of 183.6 oC of the SCW process. The characterization results confirmed that the lignocellulose structure was changed remarkably then contribute to the efficient processes of enzymatic hydrolysis. The parameters evaluation using RSM in this study suggests that SCW hydrolysis could be subjected to commercial purposes.


    Keywords: response surface methodology, subcritical water, enzymatic hydrolysis, sugar production, coconut husk

  • QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP STUDY OF ESTER-BASED FERULIC ACID DERIVATIVES AGAINST CERVICAL CANCER CELL (HELA)
    Vol 1 No 1 (2020)

    Helda Wika Amini, Istiqomah Rahmawati, Rizki Fitria Darmayanti, and Boy Arief Fachri

    Abstract

    Quantitative structure-activity relationship (QSAR) has been studied for ferulic acid derivatives to determine the QSAR model able to predict anticancer. As the subject of this research was a set of experimentally calculated IC50 value data of 6 ferulic acid derivatives against cervical cancer cells (HELA). QSAR analysis was based on multilinear regression calculation on fitting subset using log (1/IC50) as the dependent variable, and dipole moment, partition coefficient in the n-octanol/water, and atomic net charges of the aromatic carbons as independent variables. The values of the descriptors were obtained from semiempirical PM3 quantum mechanic calculation. The relationship between log (1/IC50) and the descriptors was described by the result in the QSAR model. The QSAR model for ferulic acid derivatives against HELA cell lines was developed with the statistical parameters of R=0.998; R2=0.999; SE=0.00857; and F=394. The calculated log (1/IC50) using QSAR Hansch Model for ferulic acid derivatives have excellent agreement with experimental data of Log (1/IC50).


    Keywords: QSAR Study, Ferulic Acid Derivatives, Descriptors

  • BIOBUTANOL PRODUCTION USING FED-BATCH HIGH CELL DENSITY EXTRACTIVE FERMENTATION
    Vol 1 No 1 (2020)

    Rizki Fitria Darmayanti, Yukihiro Tashiro, Kenji Sakai, and Kenji Sonomoto

    Abstract

    Butanol, as a product with specific toxicity for its producer, is necessary to be maintained in low concentration during fermentation. In-situ integrated recovery using extensive volume extraction was used in high cell density fermentation in order to prevent the lag phase in the prime condition. Clostridium saccharoperbutylacetonicum N1-4 ATCC 13564 was employed to ferment glucose in extractive fermentation with oleyl alcohol as extractant. As the results of fed-batch cultures using high cell density with different extractant to broth volume ratios (Ve/Vb), 0.8 g/l butanol concentration in the broth was maintained with a ratio of 10, which was much lower than 4.4 g/l with the ratio of 0.5. Besides, the Ve/Vb ratio of 10 demonstrated 2.7-fold higher total butanol concentration (28 g/l) than that 11 g/l obtained with a Ve/Vb ratio of 0.5. These results indicated that larger Ve/Vb improved total butanol concentration by reducing butanol toxicity in broth.

    Keywords: butanol fermentation, extraction, oleyl alcohol, tributyrin, high cell density

  • NATURAL SOURCES SCREENING FOR ANTIMICROBIAL AGENT OF HERBS, SPICES, AND EXTRACTS: A SEMI-QUALITATIVE STUDY
    Vol 1 No 1 (2020)

    Felix Arie Setiawan, Meta Fitri Rizkiana, Bekti Palupi, and Istiqomah Rahmawati

    Abstract

    Indonesia has been recognized for its rich natural ingredients such as spices, herbs, and extracts for decades. Furthermore, these components also have been used as an herbal medicine for a long time ago. Meanwhile, the apparent capability comparison of several components on antimicrobial activity has not been updated yet. This study was conducting the comparison of antimicrobial activity for several materials such as noni (Morinda citrifolia), garlic (Allium sativum), celery (Apium graveolens), galangal (Alpinia galangal), ginger (Zingiber officinale), yellow turmeric (Curcuma longa), lime (Citrus aurantifolia), papaya (Carica papaya) leaf, betel (Piper betle) leaf, and cutcherry (Kaempferia galangal) using agar dilution method. Two types of bacteria are used for the test, namely gram-negative bacteria and gram-positive bacteria, with a total of seven bacteria. The media used were TSA (Trypticase Soy Agar) media for gram-negative bacteria and MRSA (Methicillin-Resistant Staphylococcus Aureus) media for gram-positive bacteria. This study had been conducted by using a fast screening method, which referred to a semi-qualitative method. Several components, such as noni, lime, and betel leaf, showed a significant result of antimicrobial activity. Otherwise, other compounds, surprisingly, could not suppress bacterial growth.


    Keywords: herbs, spices, extracts, antimicrobial activity, and agar dilution method

  • JOURNAL OF BIOBASED CHEMICALS 2020-01 FULL
    Vol 1 No 1 (2020)

    MICROWAVE ASSISTED-EXTRACTION OF ESSENTIAL OIL FROM FRESH BASIL (OCIMUM BASILICUM L.) LEAVES
    Ditta Kharisma Yolanda Putri and Atiqa Rahmawati

    NATURAL SOURCES SCREENING FOR ANTIMICROBIAL AGENT OF HERBS, SPICES, AND EXTRACTS: A SEMI-QUALITATIVE STUDY
    Felix Arie Setiawan, Meta Fitri Rizkiana, Bekti Palupi, and Istiqomah Rahmawati

    BIOBUTANOL PRODUCTION USING FED-BATCH HIGH CELL DENSITY EXTRACTIVE FERMENTATION
    Rizki Fitria Darmayanti, Yukihiro Tashiro, Kenji Sakai, and Kenji Sonomoto

    QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP STUDY OF ESTER-BASED FERULIC ACID DERIVATIVES AGAINST CERVICAL CANCER CELL (HELA)
    Helda Wika Amini, Istiqomah Rahmawati Rahmawati, Rizki Fitria Darmayanti, and Boy Arief Fachri

    OPTIMIZATION OF SUBCRITICAL WATER ASSISTED BY NITROGEN PRIOR TO ENZYMATIC HYDROLYSIS FOR REDUCING SUGAR PRODUCTION
    Maktum Muharja, Nur Fadhilah, Rizki Fitria Darmayanti, Dwina Moentamaria, and Arief Widjaja

  • MICROWAVE ASSISTED-EXTRACTION OF ESSENTIAL OIL FROM FRESH BASIL (OCIMUM BASILICUM L.) LEAVES
    Vol 1 No 1 (2020)

    Ditta Kharisma Yolanda Putri and Atiqa Rahmawati

     

    Abstract

    In this research, the extraction of essential oil from fresh basil leaves using solvent-free microwave extraction (SFME) and microwave hydrodistillation (MHD) method. Several parameters influence the extraction of basil oil using SFME method: microwave power, the ratio between the mass of raw material with a volume of distiller (F/D), material size, and length of extraction time. Besides, the components contained in basil oil and changes in oil gland conditions in basil leaves before and after being extracted were also evaluated. The optimum condition was obtained as follows: microwave power of 380 W, the ratio between the mass of raw material with a volume of distiller (F/D) of 0.1 g/mL, raw material size of intact (± 3 cm), with an extraction time of 60 min. Moreover, SFME has a shorter extraction time to produce yields than MHD methods. GC-MS analyzed the composition of basil oil, and there were 49 identified components. This study shows that SFME method more effective than the MHD method for extraction basil oil from fresh leaves based on time extraction and yield.

    Keywords: basil oil, essential oil, microwave hydrodistillation, Ocimum basilicum L., solvent-free microwave extraction

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