Increased Plasma GLP-1 Levels after Resistant Starch Type 3 From Cassava Starch (Manihot Esculanta Crantz) Diet on Diabetic Rat

Abstract

Diabetes mellitus (DM) occur when human body is unable to produce enough insulin and/or unable to use insulin effectively, resulting in an increase of blood sugar levels (hyperglycemia). Indonesia  has  ranks  fourth  after  United  States,  China  and  India  in  the  prevalence  of  DM. Controling blood sugar and insulin levels can be done through the stimulation effect of Glucagon- like peptide-1 (GLP-1). The goal of this study was to determine the effects of resistant starch type 3 (RS3) from cassava starch in increasing plasma GLP-1 levels. This reasearch use male mouse (Ratttus novergicus) as a diabetic model by providing a high-fat diet (HFD) for one mounth than combine with intraoperitonel injection of low dose streptozotocin (STZ). The mouse then grouped into 4 groups randomly (1) normal or negative control, (2) cassava starch diet, (3) RS3 diet, (4) positive control. Blood sugar levels were measured before and after STZ injection to determine the diabetic conditions (blood sugar > 200 mg /dL). After 4 weeks of dietary administration blood sugar  and  plasma  GLP-1  levels  were  examined  using  ELISA.  Statistical  analysis  showed decreased in blood sugar levels and increased in plasma GLP-1 levels after one mounth of RS3 diet. This research shows that RS3 from cassava starch has a potential role as a nutritional therapy on diabetes mellitus condition.

Keywords: Resistant starch type 3, cassava starch, GLP-1, Blood Sugar, Diabetes Mellitus