Combination Effect of Methanol Extract of Hibiscus rosasinensis L. Leaf and Ciprofloxacin against Shigella dysentriae In Vitro

  • Billy Jusup Kurniawan Fakultas Kedokteran Universitas Negeri Jember
  • Muhammad Ali Shodikin
  • Bagus Hermansyah

Abstract

One of way to preventing bacterial resistance is a combination of natural plant products with antibiotics. Hibiscus rosasinensis leaf proved to have antibacterial activity against Shigella dysentriae. Ciprofloxacin is the first-choice antibiotic to treat Shigella dysentriae infection. The purpose of this study was to determine the effect of methanol extract of Hibiscus rosasinensis leaves and ciprofloxacin against S. dysentriae bacteria  in vitro. The type of this study was quasi experimental design with posttest only control group design using nine treatment groups (with concentration variation of 0.5 μg / mL, 1 μg / mL, 2 μg / mL, 4 μg / mL, 8 μg / mL, 16 μg / mL, 32 μg / mL, 64 μg / mL, and 128 μg / mL with 5 μg / 5 μL ciprofloxacin) and one control group (only 5 μg / 5 μL ciprofloxacin). The method used was agar well diffusion. The data was the diameter of inhibition zone around the well. The results showed that the variant of hibiscus leaf concentration could inhibit the growth of Shigella dysentriae but formed smaller inhibition zone diameter than ciprofloxacin alone.


 


Keywords: combination, hibiscus rosasinensis leaf, ciprofloxacin, Shigella dysentriae

Published
2018-06-26
How to Cite
KURNIAWAN, Billy Jusup; SHODIKIN, Muhammad Ali; HERMANSYAH, Bagus. Combination Effect of Methanol Extract of Hibiscus rosasinensis L. Leaf and Ciprofloxacin against Shigella dysentriae In Vitro. Journal of Agromedicine and Medical Sciences, [S.l.], v. 4, n. 2, p. 90 - 95, june 2018. ISSN 2460-9048. Available at: <https://jurnal.unej.ac.id/index.php/JAMS/article/view/6541>. Date accessed: 18 july 2018. doi: https://doi.org/10.19184/ams.v4i2.6541.
Section
Articles