Lethal Dose Determination of Larvacide Effect of Citrus suhuinensis Peel Extract on Aedes Aegypti Larvae

  • Wahyu Eko Prabowo Faculty of Medicine, University of Jember, Jember, Indonesia
  • Erma Sulistyaningsih Department of Parasitology, University of Jember, Jember, Indonesia
  • Dwita Aryadina Rachmawati Department of Public Health, University of Jember, Jember, Indonesia

Abstract

Dengue hemorrhagic fever (DHF) is an endemic infectious disease in Indonesia. This disease is caused by dengue virus and transmitted by Aedes aegypti as a vector. Vector control is an important approach to control DHF. Citrus suhuinensis contains active compounds such as saponins, tannins, flavonoids, and D-limonene that can kill Ae aegypti larvae. This study aimed to determine the effectivity and lethal dose of C. suhuinensis peel extract against Ae aegypti larva. This was an experimental study using randomized control-group posttest design on Ae aegypti instar III and IV larvae which were divided into 10 groups, i.e., positive control, negative control, 8 treatment groups of serial doses of 0.01, 0.02, 0.04, 0.06, 0.25, 0.50, 0.75 and 1 %. Each group consisted of 25 larvae. Observations on the larvae were conducted after 24 and 48 hours of treatment. The results showed that C. suhuinensis peel extract has larvicide effect as shown by the Lethal Dose value, i.e., LD50, LD95 and LD99 were 5.77, 9.34 and 11.39%, respectively. In conclusion, the C. suhuinensis peel extract has potential as an Ae. aegypti larvacide.

References

Adrianto, H., Yotopranoto, S., & Hamidah. (2014). Efektivitas ekstrak daun jeruk purut. Aspirator, 6(1), 1–6. https://ejournal2.litbang.kemkes.go.id/index.php/aspirator/article/view/4535

Arisanti, M., & Suryaningtyas, N. H. (2021). Kejadian Demam Berdarah Dengue (Dbd) Di Indonesia Tahun 2010-2019. Spirakel, 13(1), 34–41. https://doi.org/10.22435/spirakel.v13i1.5439

Assagaf, F., Wullur, A., & Yudistira, A. (2013). Uji toksisitas akut (lethal dose 50) ekstrak etanol daun gedi merah (Abelmoschus manihot L.) terhadap tikus putih jantan galur Wistar (Rattus norvegicus L .). Pharmacon Jurnal Ilmiah Farmasi Unsrat, 2(01), 23–28. https://doi.org/10.35799/pha.2.2013.882

BPOM. (2022). BPOM NO.10 Tahun 2022 Uji Toksisitas Praklinik secara in vivo. 490. https://peraturan.bpk.go.id/Home/Details/223969/peraturan-bpom-no-10-tahun-2022

Dania, I. A. (2016). Gambaran Penyakit dan Vektor Demam Berdarah Dengue (DBD) Perguruan tinggi di Medan, Sumatera Utara. Jurnal Warta, 48(1), 1–15. https://media.neliti.com/media/publications/290766-gambaran-penyakit-dan-vektor-demam-berda-ded3625d.pdf

Fajarani, R., Martini, & Adi, M. S. (2020). Gambaran Variasi Serotipe Virus Dengue Pada Pasien Infeksi Dengue Di Kota Semarang Tahun 2019. Jurnal Kesehatan Masyarakat, 8(1), 169–176. https://doi.org/10.14710/jkm.v8i1.26065

Kusuma, A. P., & Sukendra, D. M. (2016). Analisis Spasial Kejadian Demam Berdarah Dengue Berdasarkan Kepadatan Penduduk. Unnes Journal of Public Health, 5(1), 48. https://doi.org/10.15294/ujph.v5i1.9703

Nirma, Susilawaty, A., Ibrahim, H., & Amansyah, M. (2017). Efektivitas Larvasida Ekstrak Kulit Buah Jeruk Nipis ( Citrus aurantifolia ) Dalam Membunuh Jentik Nyamuk Aedes sp (Studi di Daerah Epidemi DBD di Wilayah Kerja Puskesmas Antang Kecamatan Manggala). HIGIENE: Jurnal Kesehatan Lingkungan, 3(2), 87–96. https://journal.uin-alauddin.ac.id/index.php/higiene/article/view/3700

Nurhaifah, D., & Sukesi, T. W. (2015). Efektivitas Air Perasan Kulit Jeruk Manis sebagai Larvasida Nyamuk Aedes aegypti. Kesmas: National Public Health Journal, 9(3), 207. https://doi.org/10.21109/kesmas.v9i3.566

Purnamasari, M. R., Sudarmaja, I. M., & Swastika, I. K. (2017). Potensi Ekstrak Etanol Daun Pandan Wangi ( Pandanus amaryllifolius roxb.) sebagai Larvasida Alami bagi Aedes aegypti. E-Jurnal Medika, 6(3), 1–8. http.//ojs.unud.ac.id/indexphp/eum

Ramayanti, I., & Febriani, R. (2016). Uji Efektivitas Larvasida Ekstrak Daun Pepaya (Carica papaya Linn) terhadap Larva Aedes aegypti. Syifa’ MEDIKA: Jurnal Kedokteran Dan Kesehatan, 6(2), 79. https://doi.org/10.32502/sm.v6i2.1383

Saleh, M., Susilawaty, A., Syarfaini, S., & Musdalifah, M. (2017). Uji Efektivitas Ekstrak Kulit Buah Jeruk Nipis (Citrus aurantifolia) Sebagai Insektisida Hayati Terhadap Nyamuk Aedes aegypti. Jurnal Kesehatan Lingkungan, 3(1), 30–36. https://journal.uin-alauddin.ac.id/index.php/higiene/article/view/2761

Sholihah, N. A., Weraman, P., & Ratu, J. M. (2020). Analisis Spasial dan Pemodelan Faktor Risiko Kejadian Demam Berdarah Dengue Tahun 2016-2018 di Kota Kupang. Jurnal Kesehatan Masyarakat Indonesia, 15(1), 52. https://doi.org/10.26714/jkmi.15.1.2020.52-61

Syamsir, S., Daramusseng, A., & Rudiman, R. (2020). Autokorelasi Spasial Demam Berdarah Dengue di Kecamatan Samarinda Utara, Kota Samarinda. Jurnal Kesehatan Lingkungan Indonesia, 19(2), 119–126. https://doi.org/10.14710/jkli.19.2.119-126

Trovancia, G., Sorisi, A., & Tuda, J. S. B. (2016). Deteksi transmisi virus dengue pada nyamuk wild Aedes Aegypti betina di Kota Manado. Jurnal E-Biomedik, 4(2). https://doi.org/10.35790/ebm.4.2.2016.14661

Yuliana, A., Rinaldi, R. A., Rahayuningsih, N., & Gustaman, F. (2021). Efektivitas Larvasida Granul Ekstrak Etanol Daun Pisang Nangka (Musa x paradisiaca L.) terhadap Larva Nyamuk Aedes aegypti. ASPIRATOR - Journal of Vector-Borne Disease Studies, 13(1), 69–78. https://doi.org/10.22435/asp.v13i1.4042

Yuliasih, Y., & Widawati, M. (2017). Aktivitas Larvasida Berbagai Pelarut pada Ekstrak Biji Kayu Besi Pantai (Pongamia pinnata) terhadap Mortalitas Larva Aedes spp. Balaba: Jurnal Litbang Pengendalian Penyakit Bersumber Binatang Banjarnegara, 13(2), 125–132. https://doi.org/10.22435/blb.v13i2.5807.125-132
Published
2023-06-30
How to Cite
PRABOWO, Wahyu Eko; SULISTYANINGSIH, Erma; RACHMAWATI, Dwita Aryadina. Lethal Dose Determination of Larvacide Effect of Citrus suhuinensis Peel Extract on Aedes Aegypti Larvae. Journal of Agromedicine and Medical Sciences, [S.l.], v. 9, n. 2, p. 70-74, june 2023. ISSN 2714-5654. Available at: <https://jurnal.unej.ac.id/index.php/JAMS/article/view/33098>. Date accessed: 13 nov. 2024. doi: https://doi.org/10.19184/ams.v9i2.33098.
Section
Original Research Articles