Formulation of Gel from Corn Silk Extract (Stigma maydis) and Burns Healing Activity

Noni Rahayu Putri; Nessa Nessa; Yoga Ramadhana

doi:10.19184/ams.v7i2.20287

Noni Rahayu Putri Sekolah Tinggi Farmasi Indonesia Perintis Padang, Padang, Indonesia
Nessa Nessa Sekolah Tinggi Farmasi Indonesia Perintis Padang, Padang, Indonesia
Yoga Ramadhana Sekolah Tinggi Farmasi Indonesia Perintis Padang, Padang, Indonesia

DOI: https://doi.org/10.19184/ams.v7i2.20287

Abstract

Corn silk (Stigma maydis) contains compounds that play a role in wound healing, such as flavonoids, saponins, tannins. This study aims to formulate a gel from corn hair extract and see its activity in healing burns. The test group was divided into four groups: group I (hot metal induction), group II (without extract ), group III (corn hair extract concentration 5%), and group IV (comparison B^®), which metal induction. The parameters observed were % wound healing, epithelialization time, and histopathology. The results of the study on the average% of burns healing rate 7, 14 and 21 days, namely group I (27,92%; 62,42%; 100%), group II (35,39%; 60,16%; 100%), group III (44,05%; 63,69%; 100%) and group IV (32,81%; 66,48%; 100%). The mean time of tissue peeling epithelialization groups I, II, and III was on day 16, and group IV was on day 17. For the histopathological results, the mean score of collagen fibers, fibroblast cells, and epithelialization was respectively group I (2,2; 2,7; 2), group II (2,8; 2,9; 2), group III (3,3; 2,9; 2,7), and Group IV (3,9; 3; 3). The results of the two-way ANOVA statistical test on % burn healing (p> 0.05) and one-way ANOVA at the time of epithelialization (p> 0.05) and the histopathological score (p <0.05) showed that group III (the corn hair extract 5 %) could influence the process of healing burns.

Keywords: Burns, Corn Silk Extract (Stigma maydis), % Burn Healing, Epithelialization Time, Histopathology

Author Biography

Nessa Nessa, Sekolah Tinggi Farmasi Indonesia Perintis Padang, Padang, Indonesia

Farmasi

References

Andriani D. dan Diah. (2018). Formulasi Esktrak Rambut Jagung (Corn Silk Zea Mays) Dalam Krim Tabir Surya Sebagai Preventif Kanker Kulit. Jurnal. Program Studi S1 Farmasi, STIKES Nasional.

Aponno, ,J.V., Paulina V.Y.Y, & Hamidah, S.S. (2014). Uji Efektivitas Sediaan Gel Ekstrak Etanol Daun Jambu Biji (Psidium guajava Linn) Terhadap Penyembuhan Luka Yang Terinfeksi Bakteri Staphylococcus aureus Pada Kelinci (Orytolagus cuniculus). Pharmacon Jurnal Ilmiah Farmasi. Manado: Program Studi Farmasi FMIPA UNSRA.

Cuttle, L., Kempfh, M., Phillips, G.E., Mill, J., Hayes, M.T., & Fraser, J.F. (2006). A porcine deep dermal partial thickness burn model with hypertrophic scarring. Burns, 32, 806-820.

Depkes RI. (1980). Kodeks Kosmetika Indonesia, Volume 1. Jakarta: Departemen Kesehatan Republik Indonesia.

Depkes RI. (1986). Sediaan Galenik. Jakarta: Depar-temen Kesehatan Republik Indonesia.

Depkes RI. (1995). Farmakope Indonesia IV.Jakarta: Departemen Kesehatan Republik Indonesia.

Depkes RI. (2013). Riset Kesehatan Dasar. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementerian KesehatanRI.

Garg, A., Anggarwal, D., Garg, S., dan Singla, A.K. 2002. Spreading of Semisolid Formulation : An Update, Pharmaceutical Technology, USA, pp. 84-104.

Harborne, J.B. 1987. Metode Fitokimia, Penuntun Cara Modern Menganalisis Tumbuhan, diterjemahkan oleh Kosasih Padmawinata. Bandung: Penerbit ITB.

Hasyim N.,Pare K.L.,Junaid I.,Kurniati A. 2012. Formulasi dan Uji Efektivitas GelLuka Bakar Ekstrak Daun CocorBebek (Kalanchoe Pinnata L) Pada Kelinci(Oryctolagus cuniculus).Majalah Farmasi dan Farmakologi.(2): 15.

Huynh-Ba K. 2008. Hand Book of Stability Testing in Pharmaceutical Development :Regulation,Methodologies, and Best Practice. New York: Spinger Science Business Media.

Khorasani, G., S.J. Hosseinimehr, M.Azadbakht, A. Zamani and M.R. Mahdavi. 2009. Aloe versus silve sulfadiazine creams for second degree burns: a randomized controlled study. Surg. Today, 39:587-591.

Kimura Y, Sumiyoshi M, Kawahira K, and Sakanaka M. 2006. Effects of Ginseng Saponins Isolated from Red Ginseng Roots on Burn Wound Healing in Mice. British Journal of Pharmacology. 148: 860-870.

Lachman, Leon. 2007. Teori dan Praktek Farmasi Industri. Jakarta: l-Press.

Lachman L, Lieberman HA, & Kaning JL. 1989. Teori dan Praktek Farmasi Industri, edisi III. Terjemahan S. Suyatmi. Jakarta: Universitas Indonesia press.

Mansur, AP, et al. 2017. Uji Efektivitas Sediaan Krim Limbah Rambut Jagung (Zea Mays L.) Terhadap Penyembuhan Luka Mencit Diabe-tes Mellitus. Hasanuddin Student Jour-nal.1(2): 144-150.

Nisa, Vina M., Zahara Meilawaty, Pudji Astuti. 2013. Efek Pemberian Ekstrak Daun Singkong (Manihot esculenta) Terhadap Proses Penyembuhan Luka Gingiva Tikus (Rattus norvegicus). Artikel Ilmiah Hasil Penelitian Mahasiswa. Fakultas Kedokteran Gigi, Universitas Jember (UNEJ).

Rahmayani A. 2007. Telaah kandungan kimia rambut jagung (Zea mays L.). Bogor: Institut Pertanian Bogor.

Rinza, R & Anindita, R. 2018. Potensi Ekstrak Kulit Jeruk Pacitan (Citrus Sinensis) Sebagai Stimulus Regenerasi Sel Pada Luka Bakar Rattus Norvegicus. Jurnal Labora Medika.2.(2):19-23.

Robinson, T. 1995. Kandungan Organik Tumbuhan Tinggi. Penerjemah: Padmawinata, K. Bandung: ITB.

Senthil P, Kumar AA, Manasa M, Kumar KA, Sravanthi K, and Deepa D. 2011. Wound Healing Activity of Alcoholic Extract of “Guazuma ulmifolia” Leaves on Albino Wistar Rats. International Journal of Pharma and Bio Sciences. 2(4): 34-38.

Sheikh AA, Sayyed Z, Siddiqui AR, Pratapwar AS, and Sheakh SS. 2011. Wound Healing Activity of Sesbania grandiflora Linn Flower Ethanolic Extract Using Excision and Incision Wound Model in Wistar Rats. International Journal of PharmTech Research. 3(2): 895-898.

Suwiti, N.K. 2010. Histologik Kulit Pasca Deteksi Kesembuhan Luka Pada Pemberian Mengkudu (Morindacitrofilia Linn). Buletin Veteriner Udayana Vol 2. Denpasar: Fakultas Kedokteran Hewan Universitas Udayana

Vincken, J.P., L. Heng, A. De Groot, & J.H. Gruppen. 2007. Saponin, klasifikasi dan kejadian di kerajaan tumbuhan. Piytochem. 68: 275-297

WHO.2014.Burns.[Online]Availableat:HYPERLINK“http://www.who.int/mediacentre/factsheet/fs365/en/#” [Accessed 1 November 2019].