Penambahan Beberapa Jenis Tepung Serangga Pada Media Perbanyakan Jamur Metarhizium anisopliae (Metsch.) Sorokin Guna Meningkatkan Virulensinya Terhadap Hama Crocidolomia pavonana Fabricius di Laboratorium

Addition of Several Types of Insect Flour to Metarhizium anisopliae (Metsch.) Sorokin Propagation Media to Increase Its Virulence Against Crocidolomia pavonana Fabricius In The Laboratory

  • Nizarrudin Aufa Program Studi Proteksi Tanaman, Fakultas Pertanian, Universitas Jember, Indonesia
  • Wildan Jadmiko Program Studi Proteksi Tanaman, Fakultas Pertanian, Universitas Jember, Indonesia

Abstract

Crocidolomia pavonana is a significant pest in the Brassicaceae family. The study aimed to determine the effect of applying several types of insect flour to M. anisopliae growth media to increase its virulence against C. pavonana pests. The experimental design in this study used a completely randomized design (C.R.D.). The treatment used was A = 25 gr corn rice medium, B = 25 gr corn rice medium + 1.25 gr Hong Kong caterpillar flour, C = 25 gr corn rice medium + 3.75 gr Hong Kong caterpillar flour, D = 25 gr corn rice medium gr + 1.25 gr Kroto flour, E = 25 gr corn rice medium + 3.75 gr Kroto flour, F = 25 gr corn rice medium + 1.25 gr cricket flour, G = 25 gr corn rice medium + 3.75 gr cricket flour. The addition of insect flour could not increase the virulence of M. anisopliae against C. pavonana pests to above 50% due to less than optimal humidity conditions for M. anisopliae. The comparison of the types of insect flour used to be added to the propagation media did not significantly affect some of the parameters that had been observed, but on average, the M. anisopliae fungi propagation medium was added with insect flour made from Hong Kong caterpillars, namely treatment B (25 gr corn rice medium + 1.25 gr Hong Kong caterpillar flour) can cause the highest mortality  against C. pavonana pests.

References

Afifah, L., Desriana, R., Kurniati, A., & Maryana, R. (2021). Viability of Entomopathogenic Fungi Metarhizium anisopliae (Metsch) Sorokin in Some Alternative Media and Different Shelf-Life. International Journal of Agriculture System, 8(2), 108–118. https://doi.org/10.20956/ijas.v8i2.2478
Agastya, I. M. I., Ameliawati, P., & Fikrinda, W. (2018). Eksplorasi dan Identifikasi Jamur Patogen Serangga di Rhizosfer Lahan Kering Kabupaten Malang. Penelitian Pertanian Terapan, 17(3), 13–17.
Akhmad Gazali. (2015). Pengendalian Hayati. Mujahid Press Bandung.
Alagappan, S., Chaliha, M., Sultanbawa, Y., Fuller, S., Hoffman, L. C. C., Netzel, G., Weber, N., Rychlik, M., Cozzolino, D., Smyth, H. E. E., & Olarte Mantilla, S. M. M. (2021). Nutritional Analysis, Volatile Composition, Antimicrobial and Antioxidant properties of Australian Green Ants (Oecophylla smaragdina). Future Foods, 3, 1–7. https://doi.org/10.1016/j.fufo.2020.100007
Amiri, B., Ibrahim, L., & Butt, T. M. (1999). Biocontrol Science and Technology Antifeedant Properties of Destruxins and their Potential Use with the Entomogenous Fungus Metarhizium anisopliae for Improved Control of Crucifer Pests. Biocontrol Science and Technology, 9(4), 487–498.
Andriani, R. (2016). Pengenalan Alat-Alat Laboratorium Mikrobiologi Untuk Mengatasi Keselamatan Kerja dan Keberhasilan Praktikum. Jurnal Mikrobiologi, 1(1).
Arsi, Pujiastuti, Y., Kusuma, S. S. H., & Gunawan, B. (2020). Eksplorasi, Isolasi dan Identifikasi Jamur Entomopatogen yang Menginfeksi Serangga Hama. Proteksi Tanaman Tropis, 2(1), 70–76. https://doi.org/10.19184/jptt.v1i2.18554
Arthurs, S., & Thomas, M. B. (2001). Effects of Temperature and Relative Humidity on Sporulation of Metarhizium anisopliae var . acridum in Mycosed Cadavers of Schistocerca gregaria. Journal of Invertebrate Pathology, 78, 59–65. https://doi.org/10.1006/jipa.2001.5050
Aryo, K., Purnomo, Wibowo, L., & Aeny, T. N. (2017). Virulensi Beberapa Isolat Metarhizium anisopliae Terhadap Ulat Grayak (Spodoptera litura F.) di Laboratorium. Jurnal Agrotek Tropika, 5(2), 96–101. https://doi.org/10.23960/jat.v5i2.1833
Astuti, P., Fitriana, Y., Wibowo, L., & Susilo, F. (2020). Pertumbuhan dan Patogenisitas Beberapa Isolat Mutan Metarhizium anisopliae Terhadap Hama Penghisap Polong (Riptortus linearis). Jurnal Agrotek Tropika, 8(2), 319–325. https://doi.org/10.23960/jat.v8i2.3908
Athanassiou, C. G., Kavallieratos, N. G., Rumbos, C. I., & Kontodimas, D. C. (2017). Influence of Temperature and Relative Humidity on the Insecticidal Efficacy of Metarhizium anisopliae against larvae of Ephestia kuehniella (Lepidoptera: Pyralidae) on Wheat. Journal of Insect Science, 17(1), 1–7. https://doi.org/10.1093/jisesa/iew107
Augustyniuk-Kram, A. (2011). The Parasite-Host System as Exemplified by The Interactions Between Entomopathogenic Fungi and Insects. Studia Ecologiae et Bioethicae, 9(1), 51–68. https://doi.org/10.21697/seb.2011.9.1.03
Avin, F. A. (2019). Easy Way to Count Spores and Prepare Spore Suspension by Hemocytometer.
Badjo, R., C.S. Rante, E.R.M. Meray, Assa, B. H., & Dien, M. F. (2015). Serangan Hama Ulat Krop (Crocidolomia pavonana F.) Pada Tanaman Kubis (Brassica oleracea var. capitata L.) di Kelurahan Kakaskasen II, Kecamatan Tomohan Utara, Kota Tomohon. Cocos, 6(14), 1–9.
Barreto, C. C., Staats, C. C., Schrank, A., & Vainstein, M. H. (2004). Distribution of Chitinases in the Entomopathogen Metarhizium anisopliae and Effect of N -Acetylglucosamine in Protein Secretion. Current Microbiology, 48, 102–107. https://doi.org/10.1007/s00284-003-4063-z
Benzertiha, A., Kiero´nczyk, B., Kołodziejski, P., Pruszy´nska–Oszmałek, E., Rawski, M., Józefiak, D., Józefiak, A., & ∗Dep. (2019). Tenebrio molitor and Zophobas morio Full-Fat Meals as Functional Feed Additives Affect Broiler Chickens ’ Growth Performance and Immune System Traits. Poultry Science, 1–11. https://doi.org/10.3382/ps/pez450
Budi, A. S., Afandhi, A., & Puspitarini, R. D. (2013). Patogenisitas Jamur Entomopatogen Beauveria bassiana Balsamo (Deuteromycetes : Moniliales) pada Larva Spodoptera litura Fabricus (Lepidoptera : Noctuidae). Jurnal HPT, 1(1), 57–65.
Datau, R., Kaligis, J. B., & Wanta, N. N. (2019). Serangan Hama Crocidolomia pavonana F. (Lepidoptera: Pyralidae) Pada Pertanaman Kubis di Rurukan, Paslaten, dan Kumelembuai Kota Tomohon. Cocos, 1(4), 1–5.
Delanuari, Y. (2017). Perlakuan Jamur Entomopatogen Metarhizium anisopliae pada Ulat Krop Kubis Crocidolomia pavonana dan Pengaruhnya Terhadap Mortalitas, Pembentukan Pupa dan Pemunculan Imago. [Skripsi].
Dhar, P., & Kaur, G. (2010). Cuticle-Degrading Proteases Produced by Metarhizium anisopliae and Their Induction in Different Media. Indian Journal of Microbiology, 50(4), 449–455. https://doi.org/10.1007/s12088-011-0098-1
Erawati, D. N., & Wardati, I. (2016). Efikasi Beberapa Formulasi Metarhizium anisopliae Terhadap Larva Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) Di Insektarium. Seminar Nasional Hasil Penelitian Dan Pengabdian Masyarakat, 1–5. https://doi.org/10.32734/jaet.v1i1.658
Fitrah, Z., Suryanti, & Netty. (2021). Uji Pertumbuhan Jamur Beauveria bassiana Pada Beberapa Media Pertumbuhan. AGrotekMAS, 2(1), 18–23.
Hamzah, Salbiah, D., & Sutikno, A. (2016). Pengaruh Media Perbanyakan Cendawan Entomopatogen Cordyceps militaris Fries Lokal terhadap Larva Oryctes rhinoceros L. di Laboratorium. J. Agrotek. Trop, 5(2), 77–83.
Herlinda, S., Darma Utama, M., Pujiastuti, Y., & Suwandi, S. (2006). Kerapatan Dan Viabilitas Spora Beauveria bassiana (Bals.) Akibat Subkultur Dan Pengayaan Media, Serta Virulensinya Terhadap Larva Plutella xylostella (Linn.). Jurnal Hama Dan Penyakit Tumbuhan Tropika, 6(2), 70–78. https://doi.org/10.23960/j.hptt.2670-78
Hlaing, N. Z., & Nway, W. N. O. (2017). Identification , Drymass and Spore Count of Entomopathogenic Metarhizium Fungi from Infected Insects. International Journal of Scientific and Research Publications, 7(11), 511–517.
Hussain, A., Rizwan-ul-Haq, M., Al-Ayedh, H., & Al-Jabr, A. (2014). Mycoinsecticides: Potential and Future Perspective. Recent Patents on Food, Nutrition & Agriculture, 6(1), 45–53. https://doi.org/10.2174/2212798406666140613113905
Ilham, N., Hendarti, I., & Ramadhan, T. H. (2019). Biologi Crocidolomia pavonana Fabricus (Lepidoptera: Pyralidae) yang Dipelihara Dengan Pakan Buatan di Laboratorium. Sains Mahasiswa Pertanian, 8(3), 1–13.
Indriyanti, D. R., Bintari, S. H., Setiati, N., Maulana, J., & Alfiyan, Z. (2021). The Density and Viability of Metarhizium anisopliae Conidia on Several Growth Media. Biosaintifika, 13(2), 237–242.
Jajić, I., Popović, A., Urošević, M., Krstović, S., Petrović, M., & Guljaš, D. (2019). Chemical Composition of Mealworm Larvae ( Tenebrio molitor ) Reared in Serbia . Contemporary Agriculture, 68(1–2), 23–27. https://doi.org/10.2478/contagri-2019-0005
Jayanegara, A., Sholikin, M. M., Sabila, D. A. N., Suharti, S., & Astuti, D. A. (2017). Lowering Chitin Content of Cricket (Gryllus assimilis) through Exoskeleton Removal and Chemical Extraction and its Utilization as a Ruminant Feed In Vitro. Pakistan Journal of Biological Sciences, 20(10), 523–529. https://doi.org/10.3923/pjbs.2017.523.529
Kansrini, Y. (2015). Uji Berbagai Jenis Media Perbanyakan Terhadap Perkembangan Jamur Beauveria bassiana di Laboratorium. Agrica Ekstensia, 9(1), 34–39.
Kapriyanto, Haryadi, N. T., & Hasjim, S. (2014). Patogenesitas Isolat Cendawan Metarhizium anisopliae Entomopatogen Terhadap Larva Uret Famili Scarabaiedae. Berkala Ilmiah Pertanian, 1(1), 1–8.
Kastilong, E. B., Lengkong, M., & Engka, R. (2021). Beauveria bassiana Bals . Terhadap Walang Sangit Leptocorisa acuta Thunb . Pada Tanaman Padi. Cocos, 8(8), 1–9.
Laksana, R. N., Himawan, T., & Choliq, F. A. (2022). Kombinasi Jamu Entomopatogen Beauveria bassiana (Balsamo) Vuillemin dengan Ekstak Daun Pepaya untuk Pengendalian Plutella xylostella Linnaeus (LEPIDOPTERA: PLUTELLIDAE). Jurnal Hama Dan Penyakit Tumbuhan, 10(2), 60–72. https://doi.org/10.21776/ub.jurnalhpt.2022.010.2.2
Lalujan, L., Djakarsi, S., Tuju, T., Rawung, D., & Sumual, M. (2017). Komposisi Kimia dan Gizi Jagung Lokal Varietas “Manado Kuning” sebagai Bahan Pangan Pengganti Beras. Jurnal Teknologi Pertanian, 8(1), 47–54.
Lina, E. C., Aarneti, A., Prijono, D., & Dadang, D. (2009). Potensi Insektisida Pasak Bumi (Eurycoma longifolia Jack) terhadap Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae). Jurnal Entomologi Indonesia, 6(1), 21–29. https://doi.org/10.5994/jei.6.1.21
Litwin, A., Nowak, M., & Ro, S. (2020). Entomopathogenic Fungi : Unconventional Applications. Rev Environ Sci Biotechnol, 19, 23–42. https://doi.org/10.1007/s11157-020-09525-1
Lutviandary, D., & Kuntjoro, S. (2020). Pengaruh Pakan Jangkrik ( Grillus mitratus ), Kroto ( Oecophyla smaragdina ), dan Ulat Hongkong ( Tenebrio molitor ) terhadap Pertambahan Berat Badan Anak Burung Walet ( Aerodramus fuchipaghus ) The Effect of Cricket ( Grillus mitratus ), Ant Egg ( Oecoph. Lentera BIO, 9(1), 23–27.
Magan, N. (2001). Improving Ecological Fitness of Biocontrol Agents (Issue iii). CAB International.
Manurung, A. A. (2020). Uji Efektifitas Jamur Entomopatogen Metarhizium anisopliae dan Beauveria bassiana Untuk Mengendalikan Hama Crocidolomia binotalis Pada Tanaman Kubis Brassica oleracea Di Laboratorium. [Skripsi].
Manurung, E. M., Tobing, M. C., Lubis, L., & Priwiratama, H. (2012). Efikasi Beberapa Formulasi Metarhizium anisopliae Terhadap Larva Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) di Insektarium. Agroekoteknologi, 1(1), 47–63.
Mondal, S., Baksi, S., Koris, A., & Vatai, G. (2016). Journey of Enzymes in Entomopathogenic Fungi. Pacific Science Review A: Natural Science and Engineering, 1–15. https://doi.org/10.1016/j.psra.2016.10.001
Montowska, M., Kowalczewski, P. Ł., Rybicka, I., & Fornal, E. (2019). Nutritional Value, Protein and Peptide Composition of Edible Cricket Powders. Food Chemistry, 289(March), 130–138. https://doi.org/10.1016/j.foodchem.2019.03.062
Mora, M. A. E., Castilho, A. M. C., & Fraga, M. E. (2017). Classification and Infection Mechanism of Entomopathogenic Fungi. Arq. Inst. Biol, 84, 1–10. https://doi.org/10.1590/1808-1657000552015
Moslim, R., & Kamarudin, N. (2014). The Use of Palm Kernel Cake in the Production of Conidia and Blastospores of Metarhizium anisopliae var. major for control of Oryctes rhinoceros. Journal of Oil Palm Research, 26(2), 133–139.
Muniappan, R., Shepard, B. M., Carner, G. R., & Ooi, P. A. C. (2012). Arthropod Pests Of Horticultural Crops in Tropical Asia. CAB International.
Mutmainnah. (2015). Perbanyakan Cendawan Entomopatogen Penicillium sp. Isolat Bone Pada Beberapa Media Tumbuh Organik. Perbal, 3(3), 1–11.
Niu, X., Xie, W., Zhang, J., & Hu, Q. (2019). Biodiversity of Entomopathogenic Fungi in the Soils of South China. Microorganisms, 7, 1–14.
Novianti, D. (2017). Efektivitas Beberapa Media Untuk Perbanyakan Jamur Metarhizium anisopliae. SainsMatika, 14(3), 81–88.
Nuraeni, Y., & Anggraeni, I. (2020). Potensi Serangga Hutan Sebagai Bahan Pangan Alternatif. Galam, 1(1), 49–60.
Nuraida dan A. Hasyim. (2009). Isolasi, Identifikasi, dan Karakterisasi Jamur Entomopatogen dari Rizosfir Pertanaman Kubis. J. Hort, 19(4), 419–432.
Ouedraogo, A., Fargues, J., Goettel, M. S., & Lomer, & C. J. (1997). Effect of Temperature on Vegetative Growth Among Isolates of Metarhizium anisopliae and M . flavoviride. Mycopathologia, 137(January), 37–43. https://doi.org/10.1023/A
Paat, F. J., & Pelealu, J. (2021). Morfologi dan Prilaku Hama Crocidolomia pavonana Pada Tanaman Kubis. Cocos, 3(1), 1–16.
Perwira, P., Purnomo, & Solikhin. (2016). Virulensi Beberapa Isolat Metarhizium anisopilae Terhadap Walang Sangit ( Leptocorisa oratorius F .) di Laboratorium. J. Agrotek Tropika, 4(2), 124–129.
Purwatiningsih, & Devara, M. (2017). Mating Behaviour of Crocidolomia pavonana F. Proceeding The 1st IBSC: Towards The Extended Use Of Basic Science For Enhancing Health, Environment, Energy And Biotechnology, 88–90.
Rahayu, S. K., Wijayanti, R., & Ns, Y. V. P. (2013). Effectiveness of Onion Ekstract for Control Cabbagehead Caterpillar ( Crocidolomia pavonana ). Agronomy Research, 2(4), 66–73.
Ramli, & Kusnara, S. T. R. (2019). Penambahan Tepung Serangga Pada Media Perbanyakan Metarhizium sp. Untuk Meningkatkan Virulensinya Terhadap Hama Belalang Padi Pandanwangi. Agroscience, 9(2), 178–188.
Robles-Acosta, I. N., Chacón-Hernández, J. C., Torres-Acosta, R. I., Landeros-Flores, J., Vanoye-Eligio, V., & Arredondo-Valdés, R. (2019). Entomopathogenic Fungi as Biological Control Agents of Phyllocoptruta oleivora (Prostigmata: Eriophyidae) Under Greenhouse Conditions. Florida Entomologist, 102(2), 303–308.
Rosmayuningsih, A., Rahardjo, B. T., & Rachmawati, R. (2014). Patogenisitas Jamur Metarhizium anisopliae Terhadap Hama Kepinding Tanah ( Stibaropus molginus ) ( Hemiptera : Cydnidae ) dari Beberapa Formulasi. HPT, 2(2), 28–37.
Rustama, M. M., Melanie, & Irawan, B. (2008). Patogenisitas Jamur Entomopatogen Metarhizium anisopliae Terhadap Crocidolomia pavonana Fab. dalam Kegiatan Studi Pengendalian Hama Terpadu Tanaman Kubis dengan Menggunkan Agensia Hayati. Laporan Akhir Penelitian Peneliti Muda (LITMUD) UNPAD Sumber Dana DIPA Universitas Padjadjaran, 394. file:///C:/Users/MUTI/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Unknown - 2008 - Laporan akhir penelitian peneliti muda (litmud) unpad sumber dana dipa unpad patogenisitas jamur entomopatogen.pdf
Santoro, P. H., Zorzetti, J., Constanski, K., & Neves, P. M. O. J. (2014). Conidial Production, Virulence, and Stress Tolerance of Beauveria bassiana Conidia After Successive in vitro Subculturing. Revista Colombiana de Entomología, 40(1), 85–90.
Saputro, T. B., Prayogo, Y., Rohman, F. L., & Alami, N. H. (2019). The Virulence Improvement of Beauveria bassiana in Infecting Cylas formicarius Modulated by Various Chitin Based Compounds. Biodiversitas, 20(9), 2486–2493. https://doi.org/10.13057/biodiv/d200909
Sari, W., & Khobir, M. L. (2019). Penambahan Tepung Serangga Pada Media Perbanyakan Untuk Meningkatkan Virulensi Beauveria bassiana Terhadap Walang Sangit. Pro-STek, 1(2), 70. https://doi.org/10.35194/prs.v1i2.823
Sembiring, J., & Prasetia, A. (2019). Pest Attack on Cabbage and Mustard Greens in Tanah Miring District, Merauke Regency, Papua Province. International Journal of Civil Engineering and Technology, 10(2), 773–782.
Septiana, E. (2015). Jamur Entomopatogen: Potensi dan Tantangan Sebagai Insektisida Alami Terhadap Serangga Perusak Tanaman dan Vektor Penyakit Manusia. BioTrends, 1(1), 28–32.
Septiani, R., Arumsari, A., & Rusnadi. (2020). Pemanfaatan Tepung Jangkrik Sebagai Nutrisi Manusia , Hewan , dan Media Pertumbuan Bakteri. Farmasi, 6(2), 450–455.
Shah, F. A., Wang, C. S., & Butt, T. M. (2005). Nutrition Influences Growth and Virulence of the Insect-Pathogenic Fungus Metarhizium anisopliae. FEMS Microbiology Letters, 251, 259–266. https://doi.org/10.1016/j.femsle.2005.08.010
Sharma, A., Shukla, A. K., & Pradesh, U. (2020). Entomopathogenic Fungi: A Potential Source for Biological Control of Insect Pests. Springer Nature Singapore Pte Ltd. https://doi.org/10.1007/978-981-15-3151-4
Sopialena. (2018). Pengendalian Hayati dengan Memberdayakan Potensi Mikroba. Mulawarman University Press.
Sulifoa, J. B., Fangupo, S., & Kant, R. (2016). Oviposition Periodicity, Egg Morphology and Life History of Large Cabbage Moth Crocidolomia pavonana Population in Samoa. 32(2), 28–34. https://doi.org/10.1071/SP16004
Sunarti. (2015). Pengamatan Hama dan Penyakit Penting Tanaman Kubis Bunga (Brassica oleracea var. botritys L.) Dataran Rendah. AGROQUA, 13(2), 74–80.
Teja, K. N. P. C., & Rahman, S. J. (2016). Characterisation and Evaluation of Metarhizium anisopliae ( Metsch .) Sorokin Strains for Their Temperature Tolerance. Mycology, 7(4), 171–179. https://doi.org/10.1080/21501203.2016.1247116
Tobing, S. S. L., Marheni, & Hasanuddin. (2015). Uji Efektivitas Metarhizium anisopliae Metch. dan Beauveria bassiana Bals. Terhadap Ulat Grayak (Spodoptera litura F.) Pada Tanaman Kedelai (Glicyne max L.) Di Rumah Kassa. Agroekoteknologi, 4(1), 1659–1665. https://doi.org/10.32734/jaet.v4i1.12885
Trizelia, Nurbailis, & Dina, E. (2013). Virulensi Berbagai Isolat Jamur Entomopatogen Metarhizium spp. Terhadap Hama Penggerek Buah Kakao Conopomorpha cramerella Snell. (Lepidoptera: Gracillariidae). J. HPT Tropika, 13(2), 151–158.
Trizelia, Sulyanti, E., & Suspalana, P. (2018). Virulensi Beberapa Isolat Cendawan Entomopatogen Metarhizium spp. terhadap Kepik Hijau Nezara viridula Linn. (Hemiptera: Pentatomidae). Pros Sem Nas Masy Biodiv Indon, 4(2), 266–269. https://doi.org/10.31604/jap.v2i2.518
Uribe, D., & Khachatourians, G. G. (2008). Identification and Characterization of an Alternative Oxidase in the Entomopathogenic Fungus Metarhizium anisopliae. Canadian Journal of Microbiology, 54(2), 119–127. https://doi.org/10.1139/W07-127
Utari, N. M. W., Sudiarta, I. P., & Bagus, I. G. N. (2015). Pengaruh Media Dan Umur Biakan Jamur Metarhizium anisopliae M. Terhadap Tingkat Kematian Larva Oryctes rhinoceros L. (Scarabaeidae ; Coleoptera). E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology), 4(2), 160–169.
Vey, A., Matha, V., & Dumas, C. (2002). Effects of the Peptide Mycotoxin Destruxin E on Insect Haemocytes and on Dynamics and Efficiency of The Multicellular Immune Reaction. Journal of Invertebrate Pathology, 80, 177–187.
Wahyudi, I. W., Sumadi, I. K., Mahardika, I. G., Komang Budaarsa, & Bidura, I. G. N. G. (2019). The Effect Kind of Nutrients on Egg (Kroto) Production and Egg Quality of Ants (Oecophylla smaragdina). International Journal of Fauna and Biological Studies, 6(4), 100–104.
Wang, C., Typas, M. A., & Butt, T. M. (2002). Detection and Characterisation of pr1 Virulent Gene Deficiencies in The Insect Pathogenic Fungus Metarhizium anisopliae. FEMS Microbiology Letters, 213, 251–255.
Widiyanti, N. uh P. M., & Muyadihardja, S. (2004). Uji Toksisitas Jamur Metharhizium anisopilae Terhadap Larva Nyamuk Aedes Aegypti. Media Litbang Kesehatan, 16(3), 25–30.
Widyawati, A., Ratna, E. S., & Prijono, D. (2012). Kepekaan Larva Crocidolomia pavonana Asal Cianjur, Jawa Barat, terhadap Tiga Jenis Insektisida. IPB University.
Published
2023-11-01
How to Cite
AUFA, Nizarrudin; JADMIKO, Wildan. Penambahan Beberapa Jenis Tepung Serangga Pada Media Perbanyakan Jamur Metarhizium anisopliae (Metsch.) Sorokin Guna Meningkatkan Virulensinya Terhadap Hama Crocidolomia pavonana Fabricius di Laboratorium. Berkala Ilmiah Pertanian, [S.l.], v. 6, n. 4, p. 215-229, nov. 2023. ISSN 2338-8331. Available at: <https://jurnal.unej.ac.id/index.php/BIP/article/view/39288>. Date accessed: 17 may 2024. doi: https://doi.org/10.19184/bip.v6i4.39288.
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