Efektivitas Isolat Nematoda Entomopatogen Steinernema sp. Produksi PPAH Kabupaten Kediri terhadap Mortalitas Larva Spodoptera frugiperda J. E. Smith
Abstract
Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) is a pest that has just entered Indonesia since 2019 and has adapted and become an important pest in corn plants. Therefore, we need a control by utilizing biological control agents that exist in nature, one of which is the entomopathogenic nematode type Steinernema sp. which has been developed by the Biological Agent Service Post (PPAH) "Sidodadi" in the Plosoklaten District, Kediri Regency. The methods used in this study were: collection and rearing of S. frugiperda pests; breeding of entomopathogenic nematodes Steinernema sp. in vivo. The application of an entomopathogenic nematode to test pathogenicity against 3rd instar S. frugiperda larvae. The application treatments used were population densities of 1.000 JI/ml, 800 JI/ml, 400 JI/ml, 200 JI/ml plus a control treatment. The study was designed with a single Completely Randomized Design (CRD) with six treatments and was repeated four times. Each replication consisted of 10 S. frugiperda larvae. Parameters observed included mortality of S. frugiperda larvae due to Steinernema sp.. Effectiveness of Steinernema sp. by calculating the LT50 value. Observation of biological symptoms of S. frugiperda infected with Steinernema sp. and calculation of the infection rate of Steinernema sp.. Data from observations were analyzed using a 5% ANOVA. If there is a significant difference, further DMRT testing is carried out. The effectiveness test was carried out by calculating the LT50 value using Finney's (1971) probit analysis. Based on observations for 120 hours, it was shown that entomopathogenic nematode PPAH isolate was effective in killing S. frugiperda larvae. PPAH isolate entomopathogenic nematode was effective in causing mortality in S. frugiperda larvae. The highest mortality value of S. frugiperda due to entomopathogenic nematode treatment was 97.5% in treatment with 800 JI/ml and 1000 JI/ml at 120 hours after application observation. The fastest LT50 value causing mortality in S. frugiperda larvae was in the 1000 JI/ml of 32.60 hours. The treatment with the longest LT50 value caused the mortality of 50% of the test insects, namely the 200 JI/ml over 95.88 hours. Observation of the infection rate showed the highest infection rate was found in the 1000 JI/ml at 24 hours after application with as many as 0.02% tails, this is because that the higher the inoculated entomopathogenic nematode population, the higher the infection rate value.
Authors who publish with this journal agree to the following terms:
1.Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2.Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3.Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
