ISOLASI DAN SELEKSI PSEUDOMONAD FLUORESCENS PADA RISOSFER PENYAMBUNGAN TOMAT
Abstract
[ENGLISH]Fluorescen pseudomonad had been isolated from the rhizosphere of grafting tomato with resisten rootstock (H 7996 and EG 203 from Asian Vegetable Research Development Center). Tomato varieties Permata and Fortuna were used as scion in grafting. Fluorescen pseudomonad was isolated on King’S B medium and used phosphate buffer 0,1 M + 0,1 % pepton. About 230 isolates of P. fluorescens were isolated from tomato rhizosphere at 14 HST and about 454 isolates at 28 HST. All isolates were tested for their capability to suppress the growth Ralstonia solanacearum in vitro. All isolates inhibited the growth of R. solanacearum with an inhibition zone of 1 mm to 7 mm or more. The mechanism growth of inhibition was bacteriostatic. About Ten isolates of P. fluorescens which had large inhibition zone, were not inhibit each other and inhibition against R. solanacearum due to nutrient competition.
Keywords : tomato; grafting; Fluorescens pseudomonad
[INDONESIAN]
Pseudomonad fluorescens diisolasi dari risosfer tomat hasil penyambungan dengan batang bawah tahan yaitu tomat H 7996 dan terung EG 203 dari Asian vegetebles Research Development Center (Taiwan). Sebagai batang atas digunakan varietas Permata dan Fortuna. Isolasi dilakukan pada media King’s B dan menggunakan buffer phospat 0,1 M + pepton 0,1 %. Sejumlah 230 isolat P. fluorescens berhasil diisolasi dari risosfer pada 14 HST dan 454 isolat pada 28 HST. Semua isolat diuji kemampuannya dalam menghambat pertumbuhan Ralstonia solanacearum secara in vitro. Semua isolat P. fluorescens mampu menghambat R. solanacearum dengan zona hambatan antara 1 mm sampai dengan lebih dari 7 mm. Semua isolat mempunyai mekanisme penghambatan bakteriostatik. Sebanyak sepuluh isolat P. fluorescens yang mempunyai daya hambat besar, tidak saling menghambat satu dengan yang lain dan penghambatan terhadap R solanacearum yang terjadi karena adanya kompetisi nutrisi.
Kata kunci: Tomat; Penyambungan; Pseudomonad fluorescens
How to citate: Nurcahyanti SD, T Arwiyanto, D Indradewa, J Widada. 2013. Isolasi dan seleksi pseudomonad fluorescens pada risosfer penyambungan tomat. Berkala Ilmiah Pertanian 1(1): 15-18
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