KARAKTERISASI BAKTERI PENYEBAB PENYAKIT HAWAR DAUN EDAMAME DI JEMBER
Abstract
[ENGLISH]
One among important diseases in Edamame soybean is bacterial bligh. The disease is relatively new and causes significant loss of soybean yield. This research was aim to know the characterize the pathogen of bacterial blight in Jember. Survey was one in several soybean field in Jember and the plant sample was culture on King's B medium to grow the pathogen. Identification was done through pysiological and biochemistry assay. The result showed that the pathogen of bacterial blight disease on Edamame soybean di Jember (Panti, Sukorambi, Wirolegi, Ajung, and Sumbersari) was Pseudomonas syringae pv. glycinea with properties as Gram negative, produce fluorescent pigment on King's B medium, grew on 20-40O C, pH range of 4.5-8.5, tolerant on 0.5-2% NaCl and also pathogenic and virulent on Edamame while reinoculated to Edamame.
Keywords: Edamame, bacterial blight, soybean
[INDONESIAN]
Salah satu penyakit penting pada kedelai khususnya edamame ialah hawar daun bakteri, penyakit ini merupakan penyakit yang relatif baru dan menyebabkan kerugian yang cukup signifikan. Penelitian ini bertujuan untuk mengetahui karakteristik bakteri penyebab penyakit hawar daun edamame di daerah Jember. Penelitian diawali dengan pengamatan gejala di lapangan di beberapa lokasi di daerah Jember. Bagian tanaman yang sakit dibawa ke laboratorium, selanjutnya dilakukan isolasi pada medium King B. Identifikasi dilakukan dengan serangkaian pengujian fisiologi dan biokimia. Berdasarkan pengujian yang telah dilakukan disimpulkan bahwa bakteri penyebab penyakit hawar daun edamame di Jember (Panti, Sukorambi, Wirolegi, Ajung, dan Sumbersari) ialah Pseudomonas syringae pv. glycinea yang bersifat gram negatif, mampu membentuk pigmen fluoresen pada medium king B, mampu tumbuh baik pada kisaran suhu 20-400 C, mempunyai kisaran pH 4,5-8,5, toleran pada kandungan NaCl 0,5-2%, serta bersifat patogenik dan virulen pada tanaman edamame.
Keywords: Edamame; Hawar Daun; Kedelai
How to citate: Masnilah R, AL Abadi, TH Astono, LQ Aini. 2013. Karakterisasi bakteri penyebab penyakit hawar daun edamame di Jember. Berkala Ilmiah Pertanian 1(1): 10-14.
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