Antibacterial Activity of Robusta Instant Coffee Powder Against Acidogenic and Aciduric Species of Streptococcus mutans Bacteria
Abstract
Streptococcus mutans is a major species that causes dental caries due to its acidogenic and aciduric properties. Coffee beans contain several compounds, such as caffeine, phenols, tannins, trigonelline, chlorogenic acid, and caffeic acid, which are known for their antibacterial properties. Robusta instant coffee powder is a stable product that is not easily contaminated with bacteria, fungi, and yeast. It is a zero-waste and soluble material. This study aimed to analyze the antibacterial activity of robusta instant coffee powder (RICP) against S. mutans. The antibacterial activity of RICP against S. mutans was tested using three antimicrobial susceptibility tests: 1) disk diffusion method, 2) minimum inhibitory concentrations (MIC), and 3) minimum bactericidal concentration (MBC). The results of the disk diffusion method test showed that the RICP concentration of 0.2 g/mL to 0.8 g/mL against S. mutans had an inhibition zone of 31.14 mm to 42.01 mm. MIC and MBC values of RICP against S. mutans were both 0.0313 g/mL. In conclusion, robusta instant coffee powder (RICP) demonstrates antibacterial activity against the acidogenic and aciduric S. mutans bacteria.
References
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Almeida, A. A. P., Farah, A., Silva, D. A., Nunan, E. A., Glória, M. B. A. (2006). Antibacterial Activity of Coffee Extracts and Selected Coffee Chemical Compounds against Enterobacteria. Journal of Agricultural and Food Chemistry, 54, 8738–8743.
Antonio, A. G., Iorio, N. L. P., Pierro, V. S. S., Candreva, M. S., Farah, A., dos Santos, K. R. N., Maia, L. C. (2011). Inhibitory Properties of Coffea canephora Extract against Oral Bacteria and Its Effect on Demineralization of Deciduous Teeth. Archives of Oral Biology, 56(6), 556-564.
Aristyawan, A. D., Noor, E. S., Suciati. (2017). Antibacterial Potential of Agelas cavernosa Sponge Ethanol Extract. Indonesian Journal of Pharmacy and Pharmaceutical Sciences, 4(1), 39-43.
Balouiri, M., Moulay, S., and Saad, K. I. (2016). Methods for in Vitro Evaluating Antimicrobial Activity: A Review. Journal of Pharmaceutical Analysis, 6(2), 71–79.
CLSI. (1998). Methods for Determining Bactericidal Activity of Antimicrobial Agents. Approved Guideline, CLSI document M26-A. Clinical and Laboratory Standards Institute, 950 West Valley Road Suite 2500, Wayne, Pennsylvania 19087, USA.
Das, K., Tiwari, R. K. S., Shrivastava, D. K. (2010). Techniques for Evaluating Medicinal Plant Products as Antimicrobial Agents: Current Methods and Future Trends. Journal of Medicinal Plants Research, 4, 104–111.
Duangjai, A., Nungruthai, S., Jukkrit, W., Atcharaporn, O., Nitra, N. and Atchariya, Y. (2016). Comparison of Antioxidants, Antimicrobial Activities and Chemical Profiles of Three Coffee (Coffea arabica L.) Aqueous Pulp Extracts. Integrative Medicine Research, 5(4), 324–331.
Hariyadi. (2013). Freeze Drying Technology: for Better Quality & Flavor of Dried Products. Foodreview Indonesia., 8(2), 52-57.
Grigg, G. (1972). Effects of Coumarin, Pyronin Y, 6,9-Dimethyl 2-Methylthiopurine and Caffeine on Excision Repair and Recombination Repair in Escherichia coli. J. Gen. Microbiol., 70, 221-230.
Hudzick, J. (2009). Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. American Society for Microbiology.
Lou, Z., Wang, H., Zhu, S., Ma, C., Wang, Z. (2011). Antibacterial Activity and Mechanism of Action of Chlorogenic Acid. Journal of Food Science, 76, M398–M403.
Marsh, P. D. (2004). Dental Plaque as A Microbial Biofilm. Caries Research, 38, 204-211.
Marsh, P. D. (2006). Dental Plaque as A Biofilm and A Microbial Community – Implications for Health and Disease. BMC Oral Health.
Marsh, P.D., Nyvad, B. (2008). The Oral Microflora and Biofilms on Teeth. in Dental Caries The Disease and Its Clinical Management. Edited by Fejerskov O, Kidd E. 164–187. Oxford: Blackwell Munksgaard.
Marsh, P. D. (1989). Host Defenses and Microbial Homeostasis: The Role of Microbial Interactions. Journal of Dental Research, 68, 1567-1575.
Monente, C., Bravo, J., Vitas, A. I., Arbillaga, L., De Peña, M. P., Cid, C. (2015). Coffee and Spent Coffee Extracts Protect against Cell Mutagens and Inhibit Growth of Food-Borne Pathogenic Microorganisms. Journal of Functional Foods,12, 365–374.
Patay, E. B, Tímea, B., Nora, P. (2016). Phytochemical Overview and Medicinal Importance of Coffea Species from The Past until Now. Asian Pacific Journal of Tropical Medicine.
Selby, C. P., Sancar, A. (1990). Molecular Mechanisms of DNA Repair Inhibition by Caffeine. Proceedings of the National Academy of Sciences, 87, 3522–3525.
Rufian-Henares, J. A., de la Cueva, S. P. (2009). Antimicrobial Activity of Coffee Melanoidins-a Study of Their Metal-Chelating Properties. Journal of Agricultural and Food Chemistry, 57, 432–438.
Setyati D, Adawiyah R., Ratnasari T., Su’udi M., Ulum F. B. (2023). Phenolic Profile and Antimicrobe of the Asplenium Nidus L. from Mount Gumitir, Jember, East Java, Indonesia. Bioedukasi, 21, 189-193.
Sulistiawati, Bambang, N., Akhyar, D. Z., Atikah, S. V. (2019). Antibacterial Effect of Semendo Coffee Beans (Coffea canephora) Extract Against Streptococcus sanguinis In Vitro Growth. Denta, Journal of Dentistry.
Tasew, T., Yalemtsehay, M., Tegenu, G., Mesfin, R. A., Bhagwan, S. C., Estifanos E., Ahmed M.M., and Hassen, M. (2020). In Vitro Antibacterial and Antioxidant Activities of Roasted and Green Coffee Beans Originating from Different Regions of Ethiopia. International Journal of Food Science.
Ulum, M. 2021. Robusta Jember Dideklarasikan Jadi Kopi Terbaik. https://surabaya.bisnis.com/read/20211002/532/1449574/robusta-jember-dideklarasikan-jadi-kopi-terbaik. Accessed July, 17th 2024.
Vu, T. T., Hyungrok, K., Vu, K. T., Quang, L. D., Hoa, T.N., Hun, K. In Seon, K., Gyung, J.C., Jin-Cheol, K. (2016). In Vitro Antibacterial Activity of Selected Medicinal Plants Traditionally Used in Vietnam against Human Pathogenic Bacteria. BMC complementary and alternative medicine.
Wang, H. Y., Qian, H., Yao, W. R. (2011). Melanoidins Produced by The Maillard Reaction: Structure and Biological Activity. Food Chemistry, 128, 573–584.
Wulandari A, Mawardi A. L., Marjanah. (2021). Potency of Makasar Fruit Extract (Brucea Javanica L. Merr) as An Antibactery of Escherichia coli. Bioedukasi, 21, 42-47.
Published
2024-10-12
How to Cite
LESTARI, Pujiana Endah et al.
Antibacterial Activity of Robusta Instant Coffee Powder Against Acidogenic and Aciduric Species of Streptococcus mutans Bacteria.
BIOEDUKASI, [S.l.], p. 340-345, oct. 2024.
ISSN 2580-0094.
Available at: <https://jurnal.unej.ac.id/index.php/BIOED/article/view/50812>. Date accessed: 21 dec. 2024.
doi: https://doi.org/10.19184/bioedu.v22i3.50812.
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https://orcid.org/0000-0003-1920-0515
