Leaf Morphological Response of Several Dendrobium Orchids due to Colchicine Application

Windhiana Windhiana; Dwi Sucianingtyas Sukamto; Fatimatuz Zuhro

doi:10.19184/bioedu.v21i3.39770

Windhiana Windhiana Argopuro PGRI University
Dwi Sucianingtyas Sukamto Argopuro PGRI University
Fatimatuz Zuhro Argopuro PGRI University

DOI: https://doi.org/10.19184/bioedu.v21i3.39770

Abstract

Dendrobium orchid is an ornamental plant that has a relatively stable market and does not depend on the season. The increasing public demand for Dendrobium orchids is an opportunity to improve the character and quality of Dendrobium orchids, one of which is by inducing mutations through the application of colchicine. This study aims to determine the effect of orchid varieties and colchicine application on the morphology of Dendrobium orchid leaves. This research was conducted at the Sriwijaya Orchid Gallery Cluster B9, Jember. This study used a 2 factorial Randomized Block Design (RBD). The first factor was the treatment of varieties consisting of Dendrobium Venus Purple Algis (ALG) and Dendrobium Transient Pink Frederika (FRD), the second factor was the treatment of colchicine concentrations (0 ppm or control, 4000 ppm, 5000 ppm and 6000 ppm). The results of this study indicated that the application of colchicine had a significant effect on several leaf morphological characters, namely leaf length, width, and color. The best leaf length was obtained at a concentration of 5000 ppm, the best leaf width was obtained at a control concentration of 0 ppm, and the most intense leaf color was obtained at a colchicine concentration of 6000 ppm.

References

Aili, Evi Nur, Respatijarti, Sugiharto, A. N. (2016). Pengaruh Pemberian Kolkisin Terhadap Penampilan Fenotip Galur Inbrida Jagung Pakan ( Zea mays L .) pada Fase Pertumbuhan Vegetatif. Jurnal Produksi Tanaman, 4(5), 370–377.

Damayanti, fitri, dan Mariska, I. (2003). Induksi Poliploidi Dengan Kolkisin Pada Hibrid F1 Hasil Persilangan Antar Spesies Pada Tanaman Panili Asal Ciamis. Berita Biologi, 6(4), 589–584.

Eigsti, OJ, Dustin, P. (1954). Colchicine in Agriculture Medicine Biologi and Chemistry.

Ermayanti, T. M., Nur Wijayanta, A., & Ratnadewi, D. (2018). Induksi Poliploidi pada Tanaman Talas (Colocasia esculenta (L.) Schott) Kultivar Kaliurang dengan Perlakuan Kolkisin secara In Vitro (In vitro Polyploid Induction on Taro (Colocasia esculenta (L.) Schott) Cultivar Kaliurang with Colchicine Treatment). Jurnal Biologi Indonesia, 14(1), 91–102.

Lim, M. S., Antony, J. J. J., Islam, S. M. S., Suhana, Z., & Sreeramanan, S. (2017). Effects of Melatonin on Colchicine-Treated PLBs of Dendrobium sonia-28 Orchid. Applied Biochemistry and Biotechnology, 181(1), 15–31. https://doi.org/10.1007/s12010-016-2196-3.

Liu, Y., Duan, S. De, Jia, Y., Hao, L. H., Xiang, D. Y., Chen, D. F., & Niu, S. C. (2023). Polyploid Induction and Karyotype Analysis of Dendrobium officinale. Horticulturae, 9(3), 1–15. https://doi.org/10.3390/horticulturae9030329.

Maghfirah, Roviq, M., & Nihayati, E. (2018). Pengaruh Pemberian Kolkisin terhadap Keragaman Pertumbuhan Dua Klon Temulawak secara In Vitro. Jurnal Produksi Tanaman, 6(6), 1000–1004.

Mahyuni, R., Girsang E.S.B, Hanafiah, D. S. (2015). Pengaruh Pemberian Kolkhisin Terhadap Morfologi dan Jumlah Kromosom Tanaman Binahong(Anredera cordifolia (Ten) Steenis). Jurna Agroteknologi, 4(1), 1815–1821.

Masruroh, M. (2018). Poliploidasi Anggrek Vanda lombokensis J. J. Sm. Menggunakan Kolkisin Secara In Vivo. Universitas Brawijaya.

Nadhira, K. (2019). Induksi Poliploid Menggunakan Kolkisin Pada Anggrek Dendrobium bifalce Lind.xDendrobium lithocola D.L.Jones & M.A.Clem. Secara In Vivo. Universitas Brawijaya.

Nengsih, W.W,Syafii, M, Saputro, N.W, Sandra, E. (2022). Studi Pengaruh Berbagai Konsentrasi Mutagen Kimia ( Kolkisin ) Terhadap Penampilan Morfologi Tanaman Anggrek Kribo ( Dendrobium spectabile ) Secara in Vitro Study of Influence of Various Concentrations of Chemica l Mutagen ( Colchicine ) on The Morphologi. Jurnal Agroteknologi, 7(1), 91–96.

Pham, P. L., Li, Y. X., Guo, H. R., Zeng, R. Z., Xie, L., Zhang, Z. S., Chen, J., Su, Q. L., & Xia, Q. (2019). Changes in morphological characteristics, regeneration ability, and polysaccharide content in tetraploid dendrobium officinale. HortScience, 54(11), 1879–1886. https://doi.org/10.21273/HORTSCI14310-19.

Pradana, D.V, dan Hartatik, S. (2019). The Effect Colchicine on Morphology of Eggplant (Solanum melongena L.). Berkala Ilmiah Pertanian, 2(4), 155–158.

Revathi, B. ., & Thomas, B. (2023). In vivo polyploidy induction in Dendrobium crumenatum through colchicine treatment. The Journal of Applied Horticulture, 24(03), 317–321. https://doi.org/10.37855/jah.2022.v24i03.56.

Suryani, D. R. (2015). Outlook Komoditas Pertanian Subsektor Hortikultura Anggrek. In Pusat Data Dan Sistem Informasi Pertanian (pp. 1–50).

Warmadewi, D. A. (2017). Buku Ajar Mutasi Genetik. In Mutasi Genetik (pp. 1–53).

Widiasteoty, D., Solvia, N., & Soedarjo, M. (2016). Potensi anggrek Dendrobium dalam Meningkatkan Variasi dan Kualitas Anggrek Bunga Potong. Jurnal Penelitian Dan Pengembangan Pertanian, 29(3), 101–106.

Yulia, N., Prihantoro, I., & Karti, P. D. M. H. (2022). Optimasi Penggunaan Mutagen Kolkisin untuk Peningkatan Produktivitas Tanaman Stylo (Stylosanthes guianesis (Aubl.)Sw.). Jurnal Ilmu Nutrisi Dan Teknologi Pakan, 20(1), 19–24.
Zakizadeh, S., Kaviani, B., & Hashemabadi, D. (2020). In vivo-induced polyploidy in Dendrobium ‘Sonia’ in a bubble bioreactor system using colchicine and oryzalin. Revista Brasileira de Botanica, 43(4), 921–932. https://doi.org/10.1007/s40415-020-00654-z

Leaf Morphological Response of Several Dendrobium Orchids due to Colchicine Application

Abstract

References

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