MORPHOLOGICAL CHARACTERIZATION OF PLANTAIN SQUIRREL (Callosciurus notatus) SPERM

  • Muhimatul Umami IAIN Syekh Nurjati Cirebon

Abstract

The plantain squirrel (Callosciurus notatus) is a rodent of the family Sciuridae. The population of plantain squirrels in Indonesia has decreased every year. The 2019 Checklist of the Mammals of Indonesia reports that the plantain squirrel is in a low concentration status; thus, accelerated efforts in wildlife conservation, including integrating technology with conservation efforts, are necessary. This study aims to determine the morphological characteristic of the plantain squirrel spermatozoa. This research consisted of several stages: anesthesia of animal samples, surgery, a maceration method, observation of the spermatozoa morphology using William's staining, and morphometry of sperm using ImageJ 1.46r software. The results show that the plantain squirrel sperm has a slightly flat head shape, falciform with one apical hook, and a not too long tail. The average sperm head length is 1.87 ± 0.30 µm, the average sperm tail length is 5.48 ± 0.95 µm.

References

Boddaert, P. (1785). Elenchus animalium, Volume 1. Rotterdam, Hake.

Breed, W. G. (2004). The spermatozoon of eurasian murine rodents: Its morphological diversity and evolution. Journal of Morphology, 261(1), 52–69. https://doi.org/10.1002/jmor.10228

Esteso, M. C., Soler, A. J., Fernández-Santos, M. R., Quintero-Moreno, A. A., & Garde, J. J. (2006). Functional significance of the sperm head morphometric size and shape for determining freezability in Iberian red deer (Cervus elaphus hispanicus) epididymal sperm samples. Journal of Andrology, 27(5), 662–670. https://doi.org/10.2164/jandrol.106.000489

Hidalgo, M., Rodríguez, I., & Dorado, J. (2006). Influence of staining and sampling procedures on goat sperm morphometry using the Sperm Class Analyzer. Theriogenology, 66(4), 996–1003. https://doi.org/10.1016/j.theriogenology.2006.02.039

Leao, D. L., Sampaio, W. V., Sousa, P. C., Moura, A. A. A., Oskam, I. C., Santos, R. R., & Domingues, S. F. S. (2020). Micromorphological and ultrastructural description of spermatozoa from squirrel monkeys (Saimiri collinsi Osgood, 1916). Zygote. https://doi.org/10.1017/S0967199419000868

Maryanto, I., Maharadatunkamsi, D., Setiawan Achmadi, A., Wiantoro, S., Sulistyadi, E., Yoneda, M., Suyanto, A., & Sugardjito, J. (2020). Checklist of the mammals of Indonesia : scientific name and distribution area table in Indonesia including CITES, IUCN and Indonesian category for conservation (Issue January).

Nor, S., Z, B., & Akbar., Z. (2001). Elevation Diversity Pattern of non-volant Small Mammals on Mount Nuang, Hulu, Langat, Selangor. Journal of Biological Sciences, 1(11), 1081–1084.

Oliveira, K. G., Leão, D. L., Almeida, D. V. C., Santos, R. R., & Domingues, S. F. S. (2015). Seminal characteristics and cryopreservation of sperm from the squirrel monkey, Saimiri collinsi. Theriogenology, 84(5), 743-749.e1. https://doi.org/10.1016/j.theriogenology.2015.04.031

Oliveira, K. G., Santos, R. R., Leão, D. L., Queiroz, H. L., Paim, F. P., Vianez-Júnior, J. L. S. G., & Domingues, S. F. S. (2016). Testicular biometry and semen characteristics in captive and wild squirrel monkey species (Saimiri sp.). Theriogenology, 86(3), 879-887.e4. https://doi.org/10.1016/j.theriogenology.2016.03.009

Ostermeier, G. C., Sargeant, G. A., Yandell, B. S., & Parrish, J. J. (2001). Measurement of bovine sperm nuclear shape using Fourier harmonic amplitudes. Journal of Andrology, 22(4), 584–594. https://doi.org/10.1002/j.1939-4640.2001.tb02218.x

Ozkavukcu, S., Erdemli, E., Isik, A., Oztuna, D., & Karahuseyinoglu, S. (2008). Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. Journal of Assisted Reproduction and Genetics, 25(8), 403–411. https://doi.org/10.1007/s10815-008-9232-3

Rijsselaere, T., Van Soom, A., Hoflack, G., Maes, D., & De Kruif, A. (2004). Automated sperm morphometry and morphology analysis of canine semen by the Hamilton-Thorne analyser. Theriogenology, 62(7), 1292–1306. https://doi.org/10.1016/j.theriogenology.2004.01.005

Saiful, A. A., & Nordin, M. (2004). Diversity and density of diurnal squirrels in a primary hill dipterocarp forest, Malaysia. Journal of Tropical Ecology, 20(1), 45–49. https://doi.org/10.1017/S0266467404006169

Sampaio, W. V., Oliveira, K. G., Leão, D. L., Caldas-Bussiere, M. C., Queiroz, H. L., Paim, F. P., Santos, R. R., & Domingues, S. F. S. (2017). Morphologic analysis of sperm from two neotropical primate species: Comparisons between the squirrel monkeys Saimiri collinsi and Saimiri vanzolinii. Zygote, 25(2), 141–148. https://doi.org/10.1017/S0967199416000411

Sanchez, M. V., Bastir, M., & Roldan, E. R. (2013). Geometric morphometrics of rodent sperm head shape. PloS One, 7(4), 1–10. https://doi.org/10.1371/Citation

Schatten, H., & Constantinescu, G. M. (2008). Comparative Reproductive Biology (First edit). Blackwell Publishing.

Sun, K., Feng, J., Jin, L., Liu, Y., & Jiang, Y. (2008). Identification of sympatric bat species by the echolocation calls. Frontiers of Biology in China, 3(2), 227–231. https://doi.org/10.1007/s11515-008-0017-y

Thorington Jr, R. W., & Hoffmann, R. (2005). Mammals of the world: A Taxonomic and Geographic Reference - Third Edition, Volume 2. In The Johns Hopkins University Press (Vol. 13, Issues 1–4, pp. 250–252).

Umami, M., Sistina, Y., & Wijayanti, G. E. (2020). In vitro spermatogenesis of shark minnow fish (Osteochilus hasselti Valenciennes 1842) as a potential fish reproductive biotechnology. IOP Conference Series: Earth and Environmental Science, 457(1). https://doi.org/10.1088/1755-1315/457/1/012081

Visco, V., Raffa, S., Elia, J., Delfino, M., Imbrogno, N., Torrisi, M. R., & Mazzilli, F. (2010). Morphological sperm defects analyzed by light microscopy and transmission electron microscopy and their correlation with sperm motility: Original article: Clinical investigation. International Journal of Urology, 17(3), 259–266. https://doi.org/10.1111/j.1442-2042.2010.02451.x

Yániz, J. L., Soler, C., & Santolaria, P. (2015). Computer-assisted sperm morphometry in mammals: A review. Animal Reproduction Science, 156(March), 1–12. https://doi.org/10.1016/j.anireprosci.2015.03.002
Published
2022-06-27
How to Cite
UMAMI, Muhimatul. MORPHOLOGICAL CHARACTERIZATION OF PLANTAIN SQUIRREL (Callosciurus notatus) SPERM. BIOEDUKASI, [S.l.], v. 20, n. 1, p. 31-35, june 2022. ISSN 2580-0094. Available at: <https://jurnal.unej.ac.id/index.php/BIOED/article/view/30928>. Date accessed: 02 oct. 2022. doi: https://doi.org/10.19184/bioedu.v20i1.30928.
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