TECHNIQUES OF PURIFICATION of ANTIGEN O ISOLATES of Escherichia coli AND IN VIVO ANALYSIS OF ANTIGEN IMMUNOGENICITY IN POULTRY
Abstract
Lipopolysaccharide (LPS) is a major surface component of most Gram-negative bacteria and is recognized by immune cells as pathogen-associated molecules. Antibodies against O antigens can provide protection against infection and vaccines have been developed by conjugating O antigens to carrier proteins to enhance immunogenicity. This study aims to produce O antigen from Escherichia coli and analyze the in vivo test results in poultry using ELISA technique. The methods used in this study were E. coli Bacterial Culture, O Antigen Purification, SDS PAGE, Inoculation on Experimental Animals, Rapid Plate Agglutination Test, and ELISA. Based on the results of the research conducted, it can be concluded that the O antigen of Escherichia coli injected into chickens can induce the formation of specific antibodies against E. coli in the blood. Specific antibodies to E. coli formed can be detected using the rapid plate agglutination test and ELISA technique.
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Figure 6 Antibody titer against O E.coli antigen by heating and acid method, ELISA cut off value = 0.51
Becton Dickinson. (2019). Tryptic Soy Broth Composition. March, 5–7.
Esfandiari, A., Widhyari, S. D., & Murtini, S. (2014). Respons Antibodi Anti ETEC K99 pada Induk Sapi Bunting Setelah Pemberian Vaksin Escherichia Coli Polivalen ( Antibody Response Against ETEC K99 in Pregnant Cows Following the Administration of Polyvalent Escherichia coli Vaccine ). Jurnal Ilmu Pertanian Indonesia (JIPI), 19(2), 85–90.
Furstenburg, D., & van Hoven, W. (1994). Condensed tannin as anti-defoliate agent against browsing by giraffe (Giraffa camelopardalis) in the Kruger National Park. Comparative Biochemistry and Physiology -- Part A: Physiology, 107(2). https://doi.org/10.1016/0300-9629(94)90402-2
Hendricks, S. B., Wulf, O. R., Hilbert, G. E., & Liddel, U. (1936). Hydrogen Bond Formation between Hydroxyl Groups and Nitrogen Atoms in Some Organic Compounds. Journal of the American Chemical Society, 58(10). https://doi.org/10.1021/ja01301a050
Mahardika, G. N., Jonas, M., Murwijati, T., Fitria, N., Suartha, I. N., Suartini, I. G. A. A., & Wibawan, I. W. T. (2016). Molecular analysis of hemagglutinin-1 fragment of avian influenza H5N1 viruses isolated from chicken farms in Indonesia from 2008 to 2010. Veterinary Microbiology, 186. https://doi.org/10.1016/j.vetmic.2016.02.023
Micoli, F., Rondini, S., Gavini, M., Pisoni, I., Lanzilao, L., Colucci, A. M., & Giannelli, C. (2014). Europe PMC Funders Group Europe PMC Funders Author Manuscripts A scalable method for O-antigen purification applied to various Salmonella serovars. 434(1), 136–145. https://doi.org/10.1016/j.ab.2012.10.038.A
Mufidah, T., Wibowo, H., & Subekti, D. T. (2015). pengembangan metode elisa dan teknik deteksi cepat dengan imunostik terhadap antibodi anti aeromonas hydrophila pada ikan MAS (Cyprinid carpio). Jurnal Riset Akuakultur, 10(4), 553. https://doi.org/10.15578/jra.10.4.2015.553-565
Rapley, R. (2005). Recombinant DNA and genetic analysis. In Principles and Techniques of Biochemistry and Molecular Biology, Sixth Edition. https://doi.org/10.1017/CBO9780511813412.007
Rosner, D., & Markowitz, G. (2013). Rosner and markowitz respond. In American Journal of Public Health (Vol. 103, Issue 5). https://doi.org/10.2105/AJPH.2013.301288
Sari, M., Kusharyoto, W., & Brazia, A. C. (2020). Profiling bioactive compounds in secondary metabolites from co-cultivation between actinomycetes and pathogenic bacteria. IOP Conference Series: Earth and Environmental Science, 439(1). https://doi.org/10.1088/1755-1315/439/1/012009
Setiawan, F., Wibowo, H., & Santoso, A. B. (2014). Karakteristik danau asal vulkanik studi kasus : danau tolire, pulau ternate Fajar Setiawan, Hendro Wibowo, Arianto Budi Santoso, Sulung Nomosatryo, dan Ivana Yuniarti. … Darat Tropis Di …, 2.
Simaremare, A. P. R., Bela, B., Yasmon, A., & Ibrahim, F. (2015). Optimizations of expression and purification of recombinant HIV-1 CRF01_AE p24 protein in Escherichia coli for development of immunodiagnostic assay. Medical Journal of Indonesia, 24(1), 14–18. https://doi.org/10.13181/mji.v24i1.1166
Sudjana, S. (2020). Progresivitas Pelindungan Terhadap Pencipta dalam Mendorong Ekonomi Kreatif di Indonesia. Jurnal Ilmiah Kebijakan Hukum, 14(2). https://doi.org/10.30641/kebijakan.2020.v14.183-200
Syndrome, S. (1995). Pendeteksian titer antibodi. 174–177.
WHO. (2004). Laboratory biosafety manual Third edition World Health Organization. World Health Organization, 1–178. https://doi.org/10.1007/SpringerReference_61629
Wulandari, T., Indrawati, A., & Pasaribu, F. H. (2019). Isolasi dan Identifikasi Aeromonas hydrophila pada Ikan Lele (Clarias gariepinus) Pertambakan Muara Jambi, Provinsi Jambi. Jurnal Medik Veteriner, 2(2), 89. https://doi.org/10.20473/jmv.vol2.iss2.2019.89-95
Zmijewski, M. E. (1993). Comments on “earnings forecasting research: its implications for capital markets research” by L. Brown. In International Journal of Forecasting (Vol. 9, Issue 3). https://doi.org/10.1016/0169-2070(93)90027-K
Figure 6 Antibody titer against O E.coli antigen by heating and acid method, ELISA cut off value = 0.51
Published
2023-02-25
How to Cite
RIFAI, Achmad Bahtiar et al.
TECHNIQUES OF PURIFICATION of ANTIGEN O ISOLATES of Escherichia coli AND IN VIVO ANALYSIS OF ANTIGEN IMMUNOGENICITY IN POULTRY.
BIOEDUKASI, [S.l.], v. 21, n. 1, p. 1-7, feb. 2023.
ISSN 2580-0094.
Available at: <https://jurnal.unej.ac.id/index.php/BIOED/article/view/28167>. Date accessed: 16 apr. 2024.
doi: https://doi.org/10.19184/bioedu.v21i1.28167.
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https://orcid.org/0000-0003-1920-0515
