PURIFICATION OF 31 AND 67 kDa PROTEIN FRACTION FROM SALIVARY GLAND OF Aedes Albopictus (SKUSE) (DIPTERA: CULLICIDAE)
Abstract
Aedes albopictus mosquito is a potential vector for Dengue Haemorrhagic Fever (DHF) which transmits Dengue virus during blood feeding. The success of the blood feeding process is aided by the biological activity of proteins in the salivary glands of Aedes albopictus. There are 30 types of proteins from the salivary glands of Aedes albopictus which are carried along blood feeding process. Proteins in the salivary glands act as vasodilator and immunomodulator. Previous studies have identified two immunogenic proteins from the salivary glands of Aedes albopictus with molecular weight of 31 and 67 kDa. Further research on the biological function of these proteins requires its purified protein to better specify the target to developing a dengue vaccine. The objective of this study was to obtain 31 and 67 kDa purified proteins by implementation of electroelution and dialysis purification. The 31 and 67 kDa protein was successfully purified by this method. This has been confirmed by a single band visualization after SDS-PAGE analysis
References
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Calvo, E., B. J. Mans, J. F. Andersen, and J. M. Ribeiro. 2006. Function and evolution of a mosquito salivary protein family. Journal of Biological Chemistry. 281(4): 1935-1942.
Calvo, E., B. J. Mans, J. M. Ribeiro, and J. F. Andersen. 2009. Multifunctionality and mechanism of ligand binding in a mosquito anti inflammatory protein. Proceedings of the National Academy of Sciences. 106(10): 3728-3733.
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Carvalho, I. D. L., D.K. Rocha and A. P. G. Almeida. 2011. Immune reactivity to dengue and Aedes albopictus mosquitoes in the population from Macao, China, before dengue occurrence. In Vivo. 25: 625-632.
Chen, W. Q., E. Karnaukhova, and G. Lubec . 2013. The use of native gels for the concomitant determination of protein sequences and modifications by mass spectrometry with subsequent conformational and functional analysis of native proteins following electro-elution. Amino Acids. 44(5) : 1381-1389.
Doucoure, S., S. Cornelie, S. Patramool, F. Mouchet, E. Demettre, M. Seveno, J. S. Dehecq, H. Rutee, J. P. Herve, F. Favier, D. Missé, P. Gasque and F. Remoue. 2013. First screening of Aedes albopictus immunogenic salivary proteins. Insect Molecular Biology. 22(4): 411-423.
Fic, E., S. Kedracka-Krok, U. Jankowska, A. Pirog, and M. Dziedzicka-Wasylewska. 2010. Comparison of protein precipitation methods for various rat brain structures prior to proteomic analysis. Electrophoresis. 31(21): 3573–3579.
Fong, S. W., R. M. Kini and L. F. P. Ng. 2018. Mosquito saliva reshapes alphavirus infection and immunopathogenesis. Journal of Virology. 92(12): 1-10.
Fontaine, A., I. Diouf, N. Bakkali, D. Missé, F. Pagès, T. Fusai, C. Rogier and L. Almeras. 2011. Implication of haematophagous arthropod salivary proteins in host-vector interactions. Parasites & Vectors. 4(187): 1-17.
Gratz, N. G. 2004. Critical review of the vector status of Aedes albopictus. Medical and Veterinary Entomology 18 : 215–227.
Gromov, P.S and J.E. Celis. 2004. Electroblotting of proteins from polyacrylamide gels. Methods in Molecular Biology (Clifton, N.J.). 244: 345–352.
Harandi, M., M. R. Ramadhian, and R. Wahyudo. 2018. DENV-5: ancaman serotipe baru virus dengue. Majority. 7(2): 1–6.
Harrington, M.G. 1990. Elution of Protein from gels. Methods in enzymology. 182 : 487-498.
Hunkapiller, M.W.E., F. Lujan, F. Ostrander and L.E. Hood. 1983. Methods Enzymol. 91: 227
Himmelfarb, J. and T. A. Ikizler. 2010. Medical progress: hemodialysis. New England Journal of Medicine. 363(19): 1833–1845.
Hussain, M., S. Munir, K. Rahim, N. H. Bashir, A. Basit, and B. Khattak. 2018. Characterization of dengue virus in Aedes aegypti and Aedes albopictus spp. of mosquitoes: a study in Khyber Pakhtunkhwa, Pakistan. Molecular Biology Research Communications. 7(2): 77–82.
James, A. A. and Rossignol, P.A. 1991. Mosquito salivary glands: parasitological and molecular aspects. Parasitology. 7(10): 267-269
Janzen, H.G. and K.A. Wright. 1971. Salivary Glands of Aedes aegypti (L.) : An Electron Microscope Study. Canadian Journal of Zoology. (49): 1-2.
Juhn, J., U. Naeem-Ullah, B. A. M. Guedes, A. Majid, J. Coleman, P. F. P. Pimenta, W. Akram., A. A. James. and O. Marinotti. 2011. Spatial mapping of gene expression in the salivary glands of the dengue vector mosquito, Aedes aegypti. Parasites & vectors. (4)1: 1.
Khasanah, R.N. 2019. Protein Imunogenik Kelenjar Saliva Aedes albopictus Vektor Potensial Demam Berdarah Dengue (DBD) di Wilayah Endemik Kabupaten Jember. Skripsi Jember : Fakultas Matematika and Ilmu Pengetahuan Alam Universitas Jember.
Lei, Z., A. Anand, K.S. Mysore and L.W. Sumner. 2007. Electroelution of intact proteins from SDS-PAGE gels and their subsequent MALDI-TOF MS analysis. Plant Proteomics: 353-363.
Li, G. Q., J. Shao, C.G. Guo, J.Y. Dong, L.Y., Fan, and C. X. Cao. 2012. A simple monolithic column electroelution for protein recovery from gel electrophoresis. Analytical biochemistry. 430(1) : 24-31.
Manning, J. E., D. M. Morens, S. Kamhawi, J. G. Valenzuela, and M. Memoli. 2018. Mosquito saliva: the hope for a universal arbovirus vaccine?. The Journal of infectious diseases. 218(1): 7-15.
Medlock J.M., D. Avenell, I. Barrass and S. Leach. 2006. Analysis of the potential for survival and seasonal sctivity of Aedes albopictus (Diptera: Culicidae) in the united kingdom. J Vector Ecol. 31: 292–304.
Mohammadian, T., M. Doosti, M. Paknejad, F. Siavoshi, and S. Massarrat. 2010. Preparative SDS-PAGE electroelution for rapid purification of alkyl hydroperoxide reductase from Helicobacter pylori. Iranian Journal of Public Health. 39(1):85–91.
Monteiro, C.C., Laura, R.B. José and D. Souza, M.R. Cleide and D. Albuquerque. 2007. Eclosion rate, development and survivorship of Aedes albopictus (Skuse) (Diptera: Culicidae) under different water temperatures. Neotropical Entomology. 36(6).
Oktarianti, R., K. Senjarini, T. Hayano, F. Fatchiyah, and Aulanni’am. 2015. Proteomic analysis of immunogenicproteins from salivary glands of Aedes aegypti. Journal of Infection and Public Health. 8(6): 575-582.
Opasawatchai, A., W. Yolwong, W. Thuncharoen, N. Inrueangsri, S. Itsaradisaikul, C. Sasisakulporn, W. Jotikasthira, O. Matangkasombut, O. Reamtong, W. Manuyakorn, W. Songnuan and P. Matangkasombut. 2020. Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients. World Allergy Organization Journal. 13(2): 1-15.
Paupy, C. , H. Delatte, L. Bagny, V. Corbel, D. Fontenille. 2009. Aedes albopictus, an arbovirus vector: from the darkness to the light. Microbes and Infection. 11 : 1177-1185.
Prasadini, M., D. Dayananda, S. Fernando, I. Harischandra, and N. De Silva, N. 2019. Blood feeding preference of female Aedes aegypti mosquitoes for human blood group types and its impact on their fecundity: implications for vector control. American Journal of Entomology. 3(2): 43-48.
Reddy, G. S. 2014. Cost-effective and efficient apparatus for electroelution of micro-and macrogram quantities of proteins from polyacrylamide gels. Preparative Biochemistry and Biotechnology. 44(8): 805–810.
Ribeiro, J. M. and Francischetti, I. M. 2003. Role of arthropod saliva in blood feeding: sialome and post-sialome perspectives. Entomol. 48: 73–88.
Rosa, E. 2007. Studi Tempat Perindukan Nyamuk Vektor Demam Berdarah Dengue di Dalam and di Luar Rumah Di Rajabasa Bandar Lampung. Jurnal Sains MIPA. 13(1).
Rueda, L. M. 2004. Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue virus transmission. Zootaxa. 589: 1–60
Sattayasai, N. 2012. Protein purification. Dalam chemical biology. Editor P. D. Ekinci and ISBN. Shanghai: In Tech.
Schmid, M. A., E. Kauffman., A. Payne., E. Harris., L. D. Kramer. 2017. Preparation of mosquito salivary gland extract and intradermal inoculation of mice. Bio-Protocol. 7 (14) : 13-16
Shoji, M., M. Kato, and S. Hashizume. 1995. Electrophoretic recovery of proteins from polyacrylamide gel. Journal of Chromatography. 698(2):145–162.
Sri-in, C., S. C. Weng, W. Y. Chen, B. A. Wu-Hsieh, W. C. Tu, and S. H. Shiao. 2019. A salivary protein of Aedes aegypti promotes dengue-2 virus replication and transmission. Insect Biochemistry and Molecular Biology. 111.
Titus R.G. and Ribeiro J.M. 1988. Salivary gland lysates from the sand fly Lutzomyia longipalpis enhance Leishmania infectivity. Science. 239(4845) : 1306-1308.
Titus, R. G., J. V. Bishop, and J. S. Mejia. 2006. The immunomodulatory factors of arthropod saliva and the potential for these factors to serve as vaccine targets to prevent pathogen transmission. Parasite Immunology. 28(4):131–141.
Vega-Rúa, A. Schmitt, C. Bonne, I. Krijnse J. Locker and A. B. Failloux. 2015. Chikungunya virus replication in salivary glands of the mosquito Aedes albopictus. Viruses. 7(11) : 5902-5907.
Wasinpiyamongkol, L., S. Patramool, S. Thongrungkiat, P. Maneekan, S. Sangmukandan, D. Missé, and N. Luplertlop. 2012. Protein expression in the salivary glands of dengue-infected Aedes aegypti mosquitoes and blood-feeding success. Southeast Asian Journal of Tropical Medicine and Public Health. 43(6): 1346-1357.
Wathon, S., F. Muti’ah., R. Oktarianti, K. Senjarini. 2020. Purifikasi protein imunogenik 31 and 56 kda dari kelenjar saliva Aedes aegypti. Jurnal Bioteknologi & Biosains Indonesia (JBBI). 7(1) : 59–71.
Wichit, S., P. Ferraris, V. Choumet and D. Misse. 2016. The effects of mosquito saliva on dengue virus infectivity in humans. Current Opinion in Virology. 21:139–145.
Vázquez-Iglesias, L. B. Estefanell-Ucha, L. Barcia-Castro, M. P. de la Cadena, P. Álvarez-Chaver, D. Ayude-Vázquez and F. J. Rodríguez-Berrocal. 2017. A simple electroelution method for rapid protein purification: isolation and antibody production of alpha toxin from Clostridium septicum. PeerJ. (5).
Wright, K.A. 1969. The Anatomy of Salivary Glands of Anopheles stephensi Liston. Canadian Journal Zoology. 47 : 579-588.
Yusoff, I.I., R. Rohani. and A.B. Mohammad. 2017. Molecular weight cut-off determination of pressure filtration membranes via colorimetric detection method. Malaysian Journal of Analytical Science. 21(2):484–495.
Zhang, H. and R. Lui. 2020. Releasing Wolbachia-infected Aedes aegypti to prevent the spread of dengue virus: a mathematical study. Infectious Disease Modelling. 5:142–160.
Published
2021-04-29
How to Cite
WATHON, Syubbanul et al.
PURIFICATION OF 31 AND 67 kDa PROTEIN FRACTION FROM SALIVARY GLAND OF Aedes Albopictus (SKUSE) (DIPTERA: CULLICIDAE).
BIOEDUKASI, [S.l.], v. 19, n. 1, p. 1-8, apr. 2021.
ISSN 2580-0094.
Available at: <https://jurnal.unej.ac.id/index.php/BIOED/article/view/18892>. Date accessed: 05 nov. 2024.
doi: https://doi.org/10.19184/bioedu.v19i1.18892.
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https://orcid.org/0000-0003-1920-0515
