Viabilitas Nematoda Entomopatogen Steinernema spp. dalam Media Kombinasi Senyawa Humik

Abstract

Its well known, That Entomopathogenic Nematods (EPNs) used as biological agents
which effective to control pest of Spodoptera litura (Noctuidae: Lepidoptera).The aims of this
research were to find out the multifungsion product as well as a biofeltilizer and biopesticide which
suitable for agriculture sustainability, secondly the materials were to mixed the humic compound and
biological agent Entomopathogenic Nematodes (EPNs). Combination of biopesticide with EPNs as
active ingredient and humic compound as biofertilizer to find out a good product combination which
indicated the highest viability and activity of EPNs Steinernema spp on humic compound, in order
application of these meterial more effective and efficient to prepare the soil fertility and pest problem.
This research was done with inoculation of EPNs on the medium/culture e.c: phosphate soluble
bacteria Pseudomonas putida 27.4B, zeolit and the humic compound (liquid culture). Pathogenecity
test of EPNs were done after incubation on the humic compound to the larvae of S. litura.
The result of this research showed that long time of viability EPNs only for fourth weeks on the humic
culture at (4000 ppm). Actually the EPNs from this incubation could 100 % mortality of the S. litura
larvae after 72 hours inoculation. The infection rate of EPNs on the larvae S. litura was 30,3 tails
during 24 hours incubation. The viability of EPNs Steinernema spp on the medium with 1000 ppm
humic compound was 20 % after 120 hours and after 4 weeks later all EPNs could not survive on this
medium. It seems, the medium containing zeolith, P. putida 27.4B and humic compound at 1000 ppm
was not suitable medium neither for nematods growth or larvae S. litura survival.