Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System

  • A. A. Istri Ratnawati
  • Wuryanti Handayani
  • Ni Nyoman Tri Puspaningsih

Abstract

A mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular β-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspelt xylan at 37oC after sixth hours. The activities of its β-endoxylanase on oat-spelt xylan was investigated. It had an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0. The enzyme was stable at 40o C for four hours and possessed a half life of four hours. β-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogram using SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates.
Published
2007-07-05
How to Cite
RATNAWATI, A. A. Istri; HANDAYANI, Wuryanti; PUSPANINGSIH, Ni Nyoman Tri. Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System. Jurnal ILMU DASAR, [S.l.], v. 8, n. 2, p. 110-117, july 2007. ISSN 2442-5613. Available at: <https://jurnal.unej.ac.id/index.php/JID/article/view/173>. Date accessed: 13 aug. 2020.
Section
General

Keywords

intestinal-system bacteria; production; characterization; β-endoxylanase